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AB16702

Anti-CD23 antibody [SP23]

  • 20ul selling size
  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • What is this?

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(8 Publications)

Anti-CD23 antibody [SP23] (ab16702) is a rabbit monoclonal antibody detecting CD23 in Western Blot, Flow Cytometry, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Trusted since 2005

View Alternative Names

CD23, CD23A, CLEC4J, FCE2, IGEBF, FCER2, Low affinity immunoglobulin epsilon Fc receptor, BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor

3 Images
Flow Cytometry - Anti-CD23 antibody [SP23] (AB16702)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD23 antibody [SP23] (AB16702)

This image was generated using a previous batch manufactured using hybridoma production method.

Flow cytometric analysis of rabbit anti-CD23 (SP23) antibody ab16702 (1/100) in Jurkats (green) compared to negative control of rabbit IgG (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] (AB16702)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] (AB16702)

Immunohistochemical analysis of paraffin-embedded Human tonsil labeling CD23 with ab16702 at 1/100 (76 μg/ml). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16702 for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Counterstained with Hematoxylin and mounted with DPX.

Western blot - Anti-CD23 antibody [SP23] (AB16702)
  • WB

Unknown

Western blot - Anti-CD23 antibody [SP23] (AB16702)

This image was generated using a previous batch manufactured using hybridoma production method.

All lanes:

Western blot - Anti-CD23 antibody [SP23] (ab16702) at 1/25 dilution

All lanes:

lysate prepared from Raji cells

Predicted band size: 36 kDa

Observed band size: 45 kDa

false

  • Carrier free

    Anti-CD23 antibody [SP23] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP23

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.</p>" } } }
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Product details

What is this antibody validated in?
Anti-CD23 antibody [SP23] (ab16702) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.

Collaboration
Anti-CD23 [SP23] (ab16702) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [SP23] also available for your convenience: ab16702, ab238780, Carrier free - ab242380, ab279476, Alexa Fluor® 750 - ab322952

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD23 also known as Fc epsilon receptor II (FcεRII) or BU38 is a type II integral membrane protein with a molecular mass of approximately 45 kDa. It is expressed mainly on B cells but also appears on various other cell types such as macrophages eosinophils and Langerhans cells. CD23 expression occurs in both soluble and membrane-bound forms. The protein consists of a lectin-like extracellular domain a short transmembrane domain and a cytoplasmic tail.
Biological function summary

CD23 facilitates several immune functions including the regulation of immunoglobulin E (IgE) production and its receptor-mediated endocytosis. It has a role in cell adhesion growth and differentiation. CD23 exists independently localizing primarily to the surface of CD23-positive cells. Furthermore this protein influences the release and activity of various cytokines impacting both innate and adaptive immunity.

Pathways

CD23 plays central roles in IgE-mediated immune responses and allergic reactions. It is importantly involved in the synthesis and regulation of IgE connecting it to the IgE signaling pathway and immune system pathways. CD23 interacts with IgE and its receptors which include the high-affinity receptor FcεRI forming an interface between the antigen and immune effector functions. Additionally CD23 participates in pathways that involve immune response modulation and inflammation prevention.

CD23 is most notably associated with allergic diseases such as asthma and allergic rhinitis due to its role in IgE regulation. It is also linked to chronic lymphocytic leukemia (CLL) in which CD23 serves as a marker for disease progression. In CLL interactions involving CD23 and related proteins such as BCMA and BAFF have been studied to better understand the disorder and its treatment. Anti-CD23 antibodies have been used as both diagnostic tools and therapeutic agents to target CD23-positive cells in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B cells. On B cells, initiates IgE-dependent antigen uptake and presentation to T cells (PubMed : 2167225). On macrophages, upon IgE binding and antigen cross-linking induces intracellular killing of parasites through activation of L-Arginine-nitric oxide pathway (PubMed : 7544003).
See full target information FCER2
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Cancers 17: PubMed40723259

2025

The Prognostic Role of Tertiary Lymphoid Structures and Immune Microenvironment Signatures in Early-Stage -Mutant Lung Adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Hsun Hsu,Chia-Chi Hsu,Min-Shu Hsieh,James Chih-Hsin Yang

World journal of surgical oncology 23:131 PubMed40205382

2025

Correlation analysis of tertiary lymphoid structure parameters with the prognosis of patients with locally advanced rectal cancer after neoadjuvant chemotherapy: a retrospective study.

Applications

Unspecified application

Species

Unspecified reactive species

Yingjian Jiang,Chuang Zhang,Yifei Hou,Bin Zhao,Binbin Cui

MedComm 5:e461 PubMed38222314

2024

Unveiling the impact of tertiary lymphoid structures on immunotherapeutic responses of clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhao Xu,Jiahe Lu,Xi Tian,Shiqi Ye,Shiyin Wei,Jun Wang,Aihetaimujiang Anwaier,Yuanyuan Qu,Wangrui Liu,Kun Chang,Hailiang Zhang,Dingwei Ye

Journal for immunotherapy of cancer 11: PubMed38040418

2023

Heterogeneity in tertiary lymphoid structures predicts distinct prognosis and immune microenvironment of clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhao Xu,Jiahe Lu,Wang-Rui Liu,Aihetaimujiang Anwaier,Yuhao Wu,Xi Tian,Jia-Qi Su,Yuan-Yuan Qu,Jianfeng Yang,Hailiang Zhang,Dingwei Ye

British journal of cancer 128:2175-2185 PubMed37016103

2023

Density and maturity of peritumoral tertiary lymphoid structures in oesophageal squamous cell carcinoma predicts patient survival and response to immune checkpoint inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshinori Hayashi,Tomoki Makino,Eiichi Sato,Kenji Ohshima,Yuya Nogi,Takashi Kanemura,Keiichiro Honma,Kotaro Yamashita,Takuro Saito,Koji Tanaka,Kazuyoshi Yamamoto,Tsuyoshi Takahashi,Yukinori Kurokawa,Hiroshi Miyata,Kiyokazu Nakajima,Hisashi Wada,Eiichi Morii,Hidetoshi Eguchi,Yuichiro Doki

Journal of pharmacological sciences 140:223-227 PubMed31353210

2019

Establishment of enzyme-linked immunosorbent facilitated antigen binding as a biomarker assay for Japanese cedar pollen allergy immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Chiharu Fukano,Katsuyo Ohashi-Doi,Kaare Lund,Atsuhito Nakao,Keisuke Masuyama,Tomokazu Matsuoka

Methods in molecular biology (Clifton, N.J.) 1845:71-86 PubMed30141008

2018

A Quantitative Pathology Approach to Analyze the Development of Human Cancer-Associated Tertiary Lymphoid Structures.

Applications

Unspecified application

Species

Unspecified reactive species

Karīna Siliņa,Chiara Burkhardt,Ruben Casanova,Alex Solterman,Maries van den Broek

Pathology research international 2011:340763 PubMed21660262

2011

Synchronous Presence of Nasopharyngeal Carcinoma and Marginal Zone (MALT-Type) B-Cell Lymphoma in the Pharynx.

Applications

Unspecified application

Species

Unspecified reactive species

Triantafyllia Koletsa,Georgios Petrakis,Georgia Karayannopoulou,Dimitrios Pappas,Konstantinos Markou,Georgios Karkavelas,Ioannis Kostopoulos
View all publications
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Product promise

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