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AB242380

Anti-CD23 antibody [SP23] - BSA and Azide free

4

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal CD23 antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CD23, CD23A, CLEC4J, FCE2, IGEBF, FCER2, Low affinity immunoglobulin epsilon Fc receptor, BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor

3 Images
Flow Cytometry - Anti-CD23 antibody [SP23] - BSA and Azide free (AB242380)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD23 antibody [SP23] - BSA and Azide free (AB242380)

Flow cytometric analysis of rabbit anti-CD23 (SP23) antibody ab16702 (1/100) in Jurkats (green) compared to negative control of rabbit IgG (blue).

This data was developed using the same antibody clone in a different buffer formulation containing Tris buffered saline, BSA, and sodium azide (ab16702).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] - BSA and Azide free (AB242380)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD23 antibody [SP23] - BSA and Azide free (AB242380)

Immunohistochemical analysis of paraffin-embedded Human tonsil labeling CD23 with ab16702 at 1/100 (76 μg/ml). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16702 for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Counterstained with Hematoxylin and mounted with DPX.

This data was developed using the same antibody clone in a different buffer formulation containing Tris buffered saline, BSA, and sodium azide (ab16702).

Western blot - Anti-CD23 antibody [SP23] - BSA and Azide free (AB242380)
  • WB

Unknown

Western blot - Anti-CD23 antibody [SP23] - BSA and Azide free (AB242380)

This image was generated using a previous batch manufactured using hybridoma production method.

This data was developed using the same antibody clone in a different buffer formulation containing Tris buffered saline, BSA, and sodium azide (ab16702).

All lanes:

Western blot - Anti-CD23 antibody [SP23] (<a href='/en-us/products/primary-antibodies/cd23-antibody-sp23-ab16702'>ab16702</a>) at 1/25 dilution

All lanes:

lysate prepared from Raji cells

Predicted band size: 36 kDa

Observed band size: 45 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP23

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.</p>" } } }
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Product details

ab242380 is the carrier-free version of ab16702.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD23 also known as Fc epsilon receptor II (FcεRII) or BU38 is a type II integral membrane protein with a molecular mass of approximately 45 kDa. It is expressed mainly on B cells but also appears on various other cell types such as macrophages eosinophils and Langerhans cells. CD23 expression occurs in both soluble and membrane-bound forms. The protein consists of a lectin-like extracellular domain a short transmembrane domain and a cytoplasmic tail.
Biological function summary

CD23 facilitates several immune functions including the regulation of immunoglobulin E (IgE) production and its receptor-mediated endocytosis. It has a role in cell adhesion growth and differentiation. CD23 exists independently localizing primarily to the surface of CD23-positive cells. Furthermore this protein influences the release and activity of various cytokines impacting both innate and adaptive immunity.

Pathways

CD23 plays central roles in IgE-mediated immune responses and allergic reactions. It is importantly involved in the synthesis and regulation of IgE connecting it to the IgE signaling pathway and immune system pathways. CD23 interacts with IgE and its receptors which include the high-affinity receptor FcεRI forming an interface between the antigen and immune effector functions. Additionally CD23 participates in pathways that involve immune response modulation and inflammation prevention.

CD23 is most notably associated with allergic diseases such as asthma and allergic rhinitis due to its role in IgE regulation. It is also linked to chronic lymphocytic leukemia (CLL) in which CD23 serves as a marker for disease progression. In CLL interactions involving CD23 and related proteins such as BCMA and BAFF have been studied to better understand the disorder and its treatment. Anti-CD23 antibodies have been used as both diagnostic tools and therapeutic agents to target CD23-positive cells in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B cells. On B cells, initiates IgE-dependent antigen uptake and presentation to T cells (PubMed : 2167225). On macrophages, upon IgE binding and antigen cross-linking induces intracellular killing of parasites through activation of L-Arginine-nitric oxide pathway (PubMed : 7544003).
See full target information FCER2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6009 PubMed40593805

2025

Spatial and single cell mapping of castleman disease reveals key stromal cell types and cytokine pathways.

Applications

Unspecified application

Species

Unspecified reactive species

David Smith,Anna Eichinger,Éanna Fennell,Zijun Y Xu-Monette,Andrew Rech,Julia Wang,Eduardo Esteva,Arta Seyedian,Xiaoxu Yang,Mei Zhang,Dan Martinez,Kai Tan,Minjie Luo,Katherine J Young,Paul G Murray,Christopher Park,Boris Reizis,Vinodh Pillai

Frontiers in bioscience (Landmark edition) 26:1411-1421 PubMed34994156

2022

ARID1A-mutated cervical cancer depends on the activation of YAP signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Gao,Peiling Li,Xianchao Kong,Tiefang Song,Qi Han,Suiyang Zhou
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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