Anti-CD24 antibody [EPR19925]

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD24 antibody [EPR19925] (AB202073)

Flow cytometric analysis of MDA-MB-231 (human breast adenocarcinoma cell line, Left) and MCF7 breast adenocarcinoma cell line, Right) cell lines labeling CD24 with ab202073 at 1/500 (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Gated on total viable cells. Negative control : MDA-MB-231 (PMID : 18433506 and 22934288).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR19925] (AB202073)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (human breast adenocarcinoma cell line) and MDA-MB-231 (human breast adenocarcinoma cell line) cells labeling CD24 with ab202073 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on MCF7 cell line.

Negative control : MDA-MB-231 (PMID : 18433506).

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

• Flow Cyt

Lab

Flow Cytometry - Anti-CD24 antibody [EPR19925] (AB202073)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab202073 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab202073 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 10⁶ in 100 µl at 5.0 μg/ml (1/400)) for 30min on ice. The cells were simultaneously stained with CD3.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed was incubated at 1/4000 for 30min on ice

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.

• Flow Cyt

Lab

Flow Cytometry - Anti-CD24 antibody [EPR19925] (AB202073)

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab202073 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab202073 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 10⁶ in 100 µl at 5.0 μg/ml (1/400)) for 30min on ice. The cells were simultaneously stained with CD19.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed was incubated at 1/4000 for 30min on ice

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.