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Rabbit Monoclonal CD24 antibody. Suitable for Flow Cyt, ICC/IF, WB and reacts with Mouse samples.

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Images

Flow Cytometry (Intracellular) - Anti-CD24 antibody [EPR26528-17] (AB290730), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CD24 antibody [EPR26528-17] (AB290730), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR26528-17] (AB290730), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR26528-17] (AB290730), expandable thumbnail
  • Western blot - Anti-CD24 antibody [EPR26528-17] (AB290730), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytICC/IFIHC-PWBIHC-Fr
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Tested
Tested
Not recommended
Tested
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Target data

Function

May have a pivotal role in cell differentiation of different cell types. May have a specific role in early thymocyte development. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses (By similarity). In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90 (PubMed:19264983). Plays a role in the control of autoimmunity (PubMed:20200274).

Alternative names

CD24, Cd24a, Ly-52, Cd24, Signal transducer CD24, Lymphocyte antigen 52, M1/69-J11D heat stable antigen, Nectadrin, R13-Ag, X62 heat stable antigen, HSA

Recommended products

Rabbit Monoclonal CD24 antibody. Suitable for Flow Cyt, ICC/IF, WB and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26528-17
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD24 also known as heat-stable antigen or CD24A is a glycosylphosphatidylinositol (GPI)-anchored membrane protein with a molecular mass of approximately 30-50 kDa. It is expressed on the surface of many cell types including B cells T cells neutrophils and epithelial cells. CD24 plays a role in cell adhesion and signaling processes impacting immune response and cancer progression. Its expression is often used as a marker in identification and classification procedures such as flow cytometry.

Biological function summary

CD24 functions like a molecular "switch" modulating signals sent between cells. It acts as a ligand for multiple receptors and participates in cellular communication especially influencing the immune system. Part of its biological role involves interaction within cellular complexes impacting immune modulation and inflammatory responses. CD24's location at the interface of cellular microenvironments makes it critical for cellular response adaptation to varied external stimuli.

Pathways

CD24 participates in both immune signaling and cancer-related pathways. It plays a significant role in the suppression of the immune response often interacting with proteins like Siglec-10 in these pathways. CD24's involvement in these pathways contributes to cell survival and proliferation particularly within cancer development. The protein’s interaction within specified signaling pathways allows it to influence downstream targets that regulate gene expression and cellular functions.

Associated diseases and disorders

CD24 has significant connections to cancer and inflammatory diseases. It is highly expressed in various cancers including breast and prostate cancer where it correlates with poor prognosis due to its role in apoptosis evasion and metastasis. CD24 interacts with proteins like HMGB1 and is implicated in inflammatory conditions where it modulates immune cell activity. Extensive research into CD24's involvement in these disorders may lead to targeted therapies including the development of anti-CD24 antibodies for therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry (Intracellular) - Anti-CD24 antibody [EPR26528-17] (ab290730), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD24 antibody [EPR26528-17] (ab290730)

    Flow cytometric analysis of Mouse PBMCs (mouse primary peripheral blood mononuclear cells) cells labelling CD24 with ab290730 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab290730. Then stained with anti-CD3 conjugated to Alexa Fluor® 647.Gated on viable cells.

  • Flow Cytometry (Intracellular) - Anti-CD24 antibody [EPR26528-17] (ab290730), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD24 antibody [EPR26528-17] (ab290730)

    Flow cytometric analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling CD24 with ab290730 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR26528-17] (ab290730), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR26528-17] (ab290730)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse PBMC (mouse primary peripheral blood mononuclear cell) cells labelling CD24 with ab290730 at 1/50 (8.56 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in subsets of mouse PBMCs is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR26528-17] (ab290730), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR26528-17] (ab290730)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling CD24 with ab290730 at 1/50 (8.56 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and membranous staining in the Neuro-2a cell line is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Western blot - Anti-CD24 antibody [EPR26528-17] (ab290730), expandable thumbnail

    Western blot - Anti-CD24 antibody [EPR26528-17] (ab290730)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-CD24 antibody [EPR26528-17] (ab290730) at 1/1000 dilution

    Lane 1: Neuro-2a (mouse neuroblastoma neuroblast) transfected with scrambled siRNA control, whole cell lysate

    Lane 2: Neuro-2a transfected with siRNA specifically targeti CD24, whole cell lysate 20

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Performed under reducing conditions.

    Observed band size: 37 kDa

    Exposure time: 3min

  • Western blot - Anti-CD24 antibody [EPR26528-17] (ab290730), expandable thumbnail

    Western blot - Anti-CD24 antibody [EPR26528-17] (ab290730)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-CD24 antibody [EPR26528-17] (ab290730) at 1/1000 dilution

    All lanes: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 37 kDa

    Exposure time: 3min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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