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AB290745

Anti-CD24 antibody [EPR26528-17] (BSA and Azide free)

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(1 Publication)

Rabbit Recombinant Monoclonal CD24 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF, WB and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD24, Cd24a, Ly-52, Cd24, Signal transducer CD24, Lymphocyte antigen 52, M1/69-J11D heat stable antigen, Nectadrin, R13-Ag, X62 heat stable antigen, HSA

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (AB290745)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (AB290745)

This data was developed using ab290730, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse PBMC (mouse primary peripheral blood mononuclear cell) cells labelling CD24 with ab290730 at 1/50 (8.56 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic staining in subsets of mouse PBMCs is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (AB290745)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (AB290745)

This data was developed using ab290730, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling CD24 with ab290730 at 1/50 (8.56 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing cytoplasmic and membranous staining in the Neuro-2a cell line is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

Flow Cytometry (Intracellular) - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (AB290745)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (AB290745)

Flow cytometric analysis of Mouse PBMCs (mouse primary peripheral blood mononuclear cells) cells labelling CD24 with ab290730 at 1/50 dilution (1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab290730. Then stained with anti-CD3 conjugated to Alexa Fluor® 647.Gated on viable cells.

Flow Cytometry (Intracellular) - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (AB290745)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (AB290745)

Flow cytometric analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling CD24 with ab290730 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

Western blot - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (AB290745)
  • WB

Supplier Data

Western blot - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (AB290745)

This data was developed using ab290730, the same antibody clone in a different buffer formulation. Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-CD24 antibody [EPR26528-17] (<a href='/en-us/products/primary-antibodies/cd24-antibody-epr26528-17-ab290730'>ab290730</a>) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast) transfected with scrambled siRNA control, whole cell lysate

Lane 2:

Neuro-2a transfected with siRNA specifically targeti CD24, whole cell lysate 20

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa

false

Exposure time: 3min

Western blot - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (AB290745)
  • WB

Supplier Data

Western blot - Anti-CD24 antibody [EPR26528-17] (BSA and Azide free) (AB290745)

This data was developed using ab290730, the same antibody clone in a different buffer formulation. Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-CD24 antibody [EPR26528-17] (<a href='/en-us/products/primary-antibodies/cd24-antibody-epr26528-17-ab290730'>ab290730</a>) at 1/1000 dilution

All lanes:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 37 kDa

false

Exposure time: 3min

  • Unconjugated

    Anti-CD24 antibody [EPR26528-17]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD24 antibody [EPR26528-17]

  • 578 PE

    PE Anti-CD24 antibody [EPR26528-17]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26528-17

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

WB, ICC/IF, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR18668] (AB189524)

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Primary Antibodies

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Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

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Primary Antibodies

AB15580

Anti-Ki67 antibody

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD24 also known as heat-stable antigen or CD24A is a glycosylphosphatidylinositol (GPI)-anchored membrane protein with a molecular mass of approximately 30-50 kDa. It is expressed on the surface of many cell types including B cells T cells neutrophils and epithelial cells. CD24 plays a role in cell adhesion and signaling processes impacting immune response and cancer progression. Its expression is often used as a marker in identification and classification procedures such as flow cytometry.
Biological function summary

CD24 functions like a molecular "switch" modulating signals sent between cells. It acts as a ligand for multiple receptors and participates in cellular communication especially influencing the immune system. Part of its biological role involves interaction within cellular complexes impacting immune modulation and inflammatory responses. CD24's location at the interface of cellular microenvironments makes it critical for cellular response adaptation to varied external stimuli.

Pathways

CD24 participates in both immune signaling and cancer-related pathways. It plays a significant role in the suppression of the immune response often interacting with proteins like Siglec-10 in these pathways. CD24's involvement in these pathways contributes to cell survival and proliferation particularly within cancer development. The protein’s interaction within specified signaling pathways allows it to influence downstream targets that regulate gene expression and cellular functions.

CD24 has significant connections to cancer and inflammatory diseases. It is highly expressed in various cancers including breast and prostate cancer where it correlates with poor prognosis due to its role in apoptosis evasion and metastasis. CD24 interacts with proteins like HMGB1 and is implicated in inflammatory conditions where it modulates immune cell activity. Extensive research into CD24's involvement in these disorders may lead to targeted therapies including the development of anti-CD24 antibodies for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May have a pivotal role in cell differentiation of different cell types. May have a specific role in early thymocyte development. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses (By similarity). In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90 (PubMed : 19264983). Plays a role in the control of autoimmunity (PubMed : 20200274).
See full target information Cd24
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Marine drugs 21: PubMed37103364

2023

The Blockade of Mitogen-Activated Protein Kinase 14 Activation by Marine Natural Product Crassolide Triggers ICD in Tumor Cells and Stimulates Anti-Tumor Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Keng-Chang Tsai,Chia-Sheng Chen,Jui-Hsin Su,Yu-Ching Lee,Yu-Hwei Tseng,Wen-Chi Wei
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com