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AB265589

Anti-CD27 antibody [BLR083G]

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(1 Publication)

Rabbit Recombinant Monoclonal CD27 antibody. Suitable for IHC-P, ICC, IP, WB, Flow Cyt and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CD27 aa 1-200.

View Alternative Names

CD27, TNFRSF7, CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7

5 Images
Immunocytochemistry - Anti-CD27 antibody [BLR083G] (AB265589)
  • ICC

Supplier Data

Immunocytochemistry - Anti-CD27 antibody [BLR083G] (AB265589)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded ramos cells labeling CD27 with ab265589 at 1/100 dilution. Detection : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [BLR083G] (AB265589)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [BLR083G] (AB265589)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD27 with ab265589 at 1/100 dilution. Detection : DAB.

Flow Cytometry - Anti-CD27 antibody [BLR083G] (AB265589)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD27 antibody [BLR083G] (AB265589)

Flow cytometry overlay histogram of 4% formaldehyde fixed Ramos cells permeabilized with 90% methanol labeling human CD27 with ab265589 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

Immunoprecipitation - Anti-CD27 antibody [BLR083G] (AB265589)
  • IP

Supplier Data

Immunoprecipitation - Anti-CD27 antibody [BLR083G] (AB265589)

CD27 was immunoprecipitated from 1mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab265589 at 20 μl/mg lysate. Western blot was performed from the immunoprecipitate using ab265589 at 1/1000 dilution.
Lane 1 : ab265589 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 10 secs.

Cells prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-CD27 antibody [BLR083G] (ab265589)

Predicted band size: 29 kDa

false

Western blot - Anti-CD27 antibody [BLR083G] (AB265589)
  • WB

Supplier Data

Western blot - Anti-CD27 antibody [BLR083G] (AB265589)

Cells prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-CD27 antibody [BLR083G] (ab265589) at 1/1000 dilution

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

Ramos whole cell lysate at 50 µg

Lane 3:

HEK-293T whole cell lysate at 50 µg

Lane 4:

Raji whole cell lysate at 50 µg

Lane 5:

Jurkat whole cell lysate at 50 µg

Lane 6:

U-2 OS whole cell lysate at 50 µg

Lane 7:

Daudi whole cell lysate at 50 µg

Lane 8:

MCF7 whole cell lysate at 50 µg

Lane 9:

CCRF-CEM whole cell lysate at 50 µg

Secondary

All lanes:

HRP conjugated goat anti-rabbit IgG

Predicted band size: 29 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR083G

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB, ICC, Flow Cyt, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CD27 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P26842

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "<p>Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with ICC staining protocol.</p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>20 μl/mg lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from TCS.
Storage buffer
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD27 also known as Tumor Necrosis Factor Receptor Superfamily Member 7 (TNFRSF7) is a protein chiefly involved in T cell activation and proliferation. It belongs to the TNF-receptor superfamily and weighs approximately 50 kDa. CD27 is expressed mainly on T lymphocytes and to a lesser extent on B cells and natural killer cells. Researchers often study CD27 and its ligand CD70 to understand immune responses. Tools such as anti-CD27 percp 7h21 monoclonal antibodies and APC-conjugated forms help detect this protein in various assays.
Biological function summary

One finds CD27 at the heart of cellular immune responses. It does not function alone; instead it typically forms part of a receptor-ligand complex with CD70 which influences T cell and B cell activities. This interaction promotes cell survival and plays a role in the activation of NF-kB and MAPK8/JNK signaling pathways which are essential for immune response regulation. These pathways ensure that the body's immune cells can efficiently respond to pathogens.

Pathways

CD27 actively integrates into the NF-kB and mTOR signaling pathways which are important for regulating immune and inflammatory responses. Through these pathways CD27 works closely with proteins like TRAF2 and TRAF3 which are adapter molecules that transmit signals from the receptor. These signaling pathways provide a framework for understanding how CD27 and associated proteins work together to control immune cell behavior facilitating coordinated immune defense mechanisms.

CD27 has significant implications in autoimmune diseases and cancer. For example aberrant activation of CD27 may contribute to conditions like lymphoma and autoimmune diseases such as lupus. The CD70-CD27 signaling axis when dysregulated can lead to uncontrolled cell proliferation or apoptosis linking it to these pathologies. Understanding the interplay between CD27 and proteins like Bcl-2 which influences apoptosis helps pave the way for therapeutic strategies targeting these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells (PubMed : 28011863). The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells (PubMed : 28011863). Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory (PubMed : 38354704). In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis (By similarity). Negatively regulates the function of Th2 lymphocytes in the adipose tissue (By similarity).
See full target information CD27
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Surgery open science 20:106-115 PubMed39021615

2024

Development and validation of a mouse model to investigate post surgical pain after laparotomy.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Martinez,Thomas Maisey,Nicola Ingram,Nikil Kapur,Paul A Beales,David G Jayne
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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