AB236155

Anti-CD27 antibody [EPR20585] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal CD27 antibody. Carrier free. Suitable for IHC-P and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD27, Tnfrsf7, Cd27, CD27 antigen, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [EPR20585] - BSA and Azide free (AB236155)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [EPR20585] - BSA and Azide free (AB236155)

Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling CD27 with ab214043 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on mouse thymus (PMID : 11728341). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214043).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [EPR20585] - BSA and Azide free (AB236155)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD27 with ab214043 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on T cells in periarterial lymphatic sheath of mouse spleen (PMID : 11782274, PMID : 11728341). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214043).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Unconjugated

Anti-CD27 antibody [EPR20585]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20585

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

ab236155 is the carrier-free version of ab214043.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD27 also known as Tumor Necrosis Factor Receptor Superfamily Member 7 (TNFRSF7) is a protein chiefly involved in T cell activation and proliferation. It belongs to the TNF-receptor superfamily and weighs approximately 50 kDa. CD27 is expressed mainly on T lymphocytes and to a lesser extent on B cells and natural killer cells. Researchers often study CD27 and its ligand CD70 to understand immune responses. Tools such as anti-CD27 percp 7h21 monoclonal antibodies and APC-conjugated forms help detect this protein in various assays.

Biological function summary

One finds CD27 at the heart of cellular immune responses. It does not function alone; instead it typically forms part of a receptor-ligand complex with CD70 which influences T cell and B cell activities. This interaction promotes cell survival and plays a role in the activation of NF-kB and MAPK8/JNK signaling pathways which are essential for immune response regulation. These pathways ensure that the body's immune cells can efficiently respond to pathogens.

Pathways

CD27 actively integrates into the NF-kB and mTOR signaling pathways which are important for regulating immune and inflammatory responses. Through these pathways CD27 works closely with proteins like TRAF2 and TRAF3 which are adapter molecules that transmit signals from the receptor. These signaling pathways provide a framework for understanding how CD27 and associated proteins work together to control immune cell behavior facilitating coordinated immune defense mechanisms.

CD27 has significant implications in autoimmune diseases and cancer. For example aberrant activation of CD27 may contribute to conditions like lymphoma and autoimmune diseases such as lupus. The CD70-CD27 signaling axis when dysregulated can lead to uncontrolled cell proliferation or apoptosis linking it to these pathologies. Understanding the interplay between CD27 and proteins like Bcl-2 which influences apoptosis helps pave the way for therapeutic strategies targeting these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells (PubMed : 11062504). Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory (By similarity). In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis (PubMed : 15723067). Negatively regulates the function of Th2 lymphocytes in the adipose tissue (PubMed : 38386557).

See full target information Cd27

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7099 PubMed36402816

2022

The non-catalytic role of DNA polymerase epsilon in replication initiation in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sameera Vipat,Dipika Gupta,Sagun Jonchhe,Hele Anderspuk,Eli Rothenberg,Tatiana N Moiseeva

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD27 antibody [EPR20585] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [EPR20585] - BSA and Azide free (AB236155)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [EPR20585] - BSA and Azide free (AB236155)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

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Properties and storage information

Form

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Shipped at conditions

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Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise