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Rabbit Recombinant Monoclonal CD27 antibody. Suitable for Flow Cyt and reacts with Human samples.

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Images

Key facts

Isotype

IgG1

Host species

Rabbit

Storage buffer

Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow Cyt
Human
Not recommended
Tested
Baboon
Not recommended
Predicted
Gorilla
Not recommended
Predicted
Macaque monkey
Not recommended
Predicted

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species

Baboon

Dilution info

-

Notes

-

Species

Macaque monkey

Dilution info

-

Notes

-

Species

Gorilla

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10

Notes

-

Predicted
Predicted

Species

Baboon, Macaque monkey, Gorilla

Dilution info

-

Notes

-

Target data

Function

Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Alternative names

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Rabbit Recombinant Monoclonal CD27 antibody. Suitable for Flow Cyt and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EPR21209

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is an in vitro raised antibody. It was made using synthetic libraries and phage display technology.

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

Supplementary info

Biological summary

One finds CD27 at the heart of cellular immune responses. It does not function alone; instead it typically forms part of a receptor-ligand complex with CD70 which influences T cell and B cell activities. This interaction promotes cell survival and plays a role in the activation of NF-kB and MAPK8/JNK signaling pathways which are essential for immune response regulation. These pathways ensure that the body's immune cells can efficiently respond to pathogens.

Mechanical summary

CD27 also known as Tumor Necrosis Factor Receptor Superfamily Member 7 (TNFRSF7) is a protein chiefly involved in T cell activation and proliferation. It belongs to the TNF-receptor superfamily and weighs approximately 50 kDa. CD27 is expressed mainly on T lymphocytes and to a lesser extent on B cells and natural killer cells. Researchers often study CD27 and its ligand CD70 to understand immune responses. Tools such as anti-CD27 percp 7h21 monoclonal antibodies and APC-conjugated forms help detect this protein in various assays.

Pathway

CD27 actively integrates into the NF-kB and mTOR signaling pathways which are important for regulating immune and inflammatory responses. Through these pathways CD27 works closely with proteins like TRAF2 and TRAF3 which are adapter molecules that transmit signals from the receptor. These signaling pathways provide a framework for understanding how CD27 and associated proteins work together to control immune cell behavior facilitating coordinated immune defense mechanisms.

Disease

CD27 has significant implications in autoimmune diseases and cancer. For example aberrant activation of CD27 may contribute to conditions like lymphoma and autoimmune diseases such as lupus. The CD70-CD27 signaling axis when dysregulated can lead to uncontrolled cell proliferation or apoptosis linking it to these pathologies. Understanding the interplay between CD27 and proteins like Bcl-2 which influences apoptosis helps pave the way for therapeutic strategies targeting these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Flow Cytometry - Anti-CD27 antibody [EPR21209] (ab213318), expandable thumbnail

    Flow Cytometry - Anti-CD27 antibody [EPR21209] (ab213318)

    Overlay histogram showing Daudi cells stained with ab213318 (red line).The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab213318, 1/10 dilution) for 30 min at 4°C. The secondary antibody (black line) used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/2000 dilution for 30 min at 4°C. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >30,000 total events were collected.

  • Flow Cytometry - Anti-CD27 antibody [EPR21209] (ab213318), expandable thumbnail

    Flow Cytometry - Anti-CD27 antibody [EPR21209] (ab213318)

    Flow cytometry analysis of Human PBMC (human peripheral blood mononuclear cell) cells staining with ab213318 at 1/500 dilution. Secondary used was Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution, and isotype control was Rabbit monoclonal IgG (ab172730) / Left. Gated on viable cell.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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