Anti-CD27 antibody [EPR27428-9] - BSA and Azide free

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD27 antibody [EPR27428-9] - BSA and Azide free (AB316960)

This data was developed using ab316959, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a mouse CD27 expression vector containing a myc-His-tag® (Middle) / 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling CD27 with ab316959 at 1/5000 dilution (0.01ug) / Middle and Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with Myc tag conjugated to Alexa Fluor®647.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD27 antibody [EPR27428-9] - BSA and Azide free (AB316960)

This data was developed using ab316959, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD27 with ab316959 at 1/500 dilution (0.1ug)/right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti CD11b conjugated to BV510.
Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD27 antibody [EPR27428-9] - BSA and Azide free (AB316960)

This data was developed using ab316959, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CD27 with ab316959 at 1/500 dilution (0.1ug)/right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti CD3 conjugated to Alexa Fluor®647.
Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD27 antibody [EPR27428-9] - BSA and Azide free (AB316960)

This data was developed using ab316959, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse PBMC cells labelling CD27 with ab316959 at 1/500 dilution (0.1ug)/right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti CD11b conjugated to BV510.
Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD27 antibody [EPR27428-9] - BSA and Azide free (AB316960)

This data was developed using ab316959, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse PBMC cells labelling CD27 with ab316959 at 1/500 dilution (0.1ug)/right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti CD3 conjugated to Alexa Fluor®647.
Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD27 antibody [EPR27428-9] - BSA and Azide free (AB316960)

This data was developed using ab316959, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Rat PBMC cells labelling CD27 with ab316959 at 1/500 dilution (0.1ug)/right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control.

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti CD3 conjugated to Alexa Fluor®647.
Gated on viable cells.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody [EPR27428-9] - BSA and Azide free (AB316960)

This data was developed using ab316959, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse PBMC (Mouse primary peripheral blood mononuclear cell) cells labelling CD27 with ab316959 at 1/50 (9.94 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in subsets of mouse PBMCs (shown in green), which is partly co-localised with CD3(shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

Anti-rat CD3 mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain at 1/100 (5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody [EPR27428-9] - BSA and Azide free (AB316960)

This data was developed using ab316959, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse PBMC (Mouse primary peripheral blood mononuclear cell) cells labelling CD27 with ab316959 at 1/50 (9.94 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing cytoplasmic staining in subsets of mouse PBMCs (shown in green), which is not co-localised with CD11b (shown in magenta). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab8878 Anti-CD11b Rat monoclonal antibody was used to counterstain at 1/200 (2.5ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.