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Rabbit Recombinant Monoclonal CD27 antibody. Suitable for WB, ICC/IF and reacts with Human samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IF
Human
Not recommended
Tested
Not recommended
Tested
Rat
Not recommended
Predicted
Not recommended
Predicted

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Target data

Function

Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Alternative names

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Rabbit Recombinant Monoclonal CD27 antibody. Suitable for WB, ICC/IF and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EPR8568

Purification technique

Affinity purification Protein A

Dissociation constant

7.6 x 10-13 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological summary

One finds CD27 at the heart of cellular immune responses. It does not function alone; instead it typically forms part of a receptor-ligand complex with CD70 which influences T cell and B cell activities. This interaction promotes cell survival and plays a role in the activation of NF-kB and MAPK8/JNK signaling pathways which are essential for immune response regulation. These pathways ensure that the body's immune cells can efficiently respond to pathogens.

Mechanical summary

CD27 also known as Tumor Necrosis Factor Receptor Superfamily Member 7 (TNFRSF7) is a protein chiefly involved in T cell activation and proliferation. It belongs to the TNF-receptor superfamily and weighs approximately 50 kDa. CD27 is expressed mainly on T lymphocytes and to a lesser extent on B cells and natural killer cells. Researchers often study CD27 and its ligand CD70 to understand immune responses. Tools such as anti-CD27 percp 7h21 monoclonal antibodies and APC-conjugated forms help detect this protein in various assays.

Pathway

CD27 actively integrates into the NF-kB and mTOR signaling pathways which are important for regulating immune and inflammatory responses. Through these pathways CD27 works closely with proteins like TRAF2 and TRAF3 which are adapter molecules that transmit signals from the receptor. These signaling pathways provide a framework for understanding how CD27 and associated proteins work together to control immune cell behavior facilitating coordinated immune defense mechanisms.

Disease

CD27 has significant implications in autoimmune diseases and cancer. For example aberrant activation of CD27 may contribute to conditions like lymphoma and autoimmune diseases such as lupus. The CD70-CD27 signaling axis when dysregulated can lead to uncontrolled cell proliferation or apoptosis linking it to these pathologies. Understanding the interplay between CD27 and proteins like Bcl-2 which influences apoptosis helps pave the way for therapeutic strategies targeting these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-CD27 antibody [EPR8568] (ab133761), expandable thumbnail

    Western blot - Anti-CD27 antibody [EPR8568] (ab133761)

    ab133761 was shown to react with CD27 in wild-type Raji cells in western blot. Loss of signal was observed when CD27 knockout cell line ab274910 (knockout cell lysate ab281360) was used. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab133761 overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1 in 5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.

    All lanes: Western blot - Anti-CD27 antibody [EPR8568] (AB133761) at 1/1000 dilution

    Lane 1: Wild-type Raji cell lysate at 20 µg

    Lane 2: CD27 knockout Raji cell lysate at 20 µg

    Lane 3: Ramos cell lysate at 20 µg

    Lane 4: Human Brain tissue lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 29 kDa

    Observed band size: 50 kDa

    Exposure time: 2min

  • Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody [EPR8568] (ab133761), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody [EPR8568] (ab133761)

    Immunofluorescent staining of CD27 in Ramos cells, using ab133761 at a 1/50 dilution.

  • Western blot - Anti-CD27 antibody [EPR8568] (ab133761), expandable thumbnail

    Western blot - Anti-CD27 antibody [EPR8568] (ab133761)

    All lanes: Western blot - Anti-CD27 antibody [EPR8568] (AB133761) at 1/1000 dilution

    All lanes: Ramos cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

    Predicted band size: 29 kDa

  • OI-RD Scanning - Anti-CD27 antibody [EPR8568] (ab133761), expandable thumbnail

    OI-RD Scanning - Anti-CD27 antibody [EPR8568] (ab133761)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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