Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free
Be the first to review this product! Submit a review
|
(0 Publication)
Mouse Monoclonal CD27 antibody. Carrier free. Suitable for Flow Cyt, Protein Array, ICC/IF, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CD27.
View Alternative Names
CD27, TNFRSF7, CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (AB268268)
Formalin-fixed, paraffin-embedded human spleen tissue stained for CD27 using ab268144 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268144)
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (AB268268)
Immunocytochemistry/ Immunofluorescence staining of Ramos (Human Burkitt's lymphoma cell line) cells staind for CD27 using ab268144 at 2μg/ml followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268144)
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (AB268268)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD27 using ab268144 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268144)
- Flow Cyt
Unknown
Flow Cytometry - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (AB268268)
Flow cytometric analysis of Ramos (Human Burkitt's lymphoma cell line) cells labeling CD27 using ab268144 at 2 μg/million cells followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268144)
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (AB268268)
Formalin-fixed, paraffin-embedded human colon tissue stained for CD27 using ab268144 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268144)
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (AB268268)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD27 using ab268144 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268144)
- Protein Array
Unknown
Protein Array - Anti-CD27 antibody [LPFS2/1611] - BSA and Azide free (AB268268)
Protein Array containing more than 19,000 full-length human proteins using ab268144.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (ab268144)
Reactivity data
You may be interested in:
Why is this recommended?
We recommend this product because it’s often used in the same experiment or related research.
We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
One finds CD27 at the heart of cellular immune responses. It does not function alone; instead it typically forms part of a receptor-ligand complex with CD70 which influences T cell and B cell activities. This interaction promotes cell survival and plays a role in the activation of NF-kB and MAPK8/JNK signaling pathways which are essential for immune response regulation. These pathways ensure that the body's immune cells can efficiently respond to pathogens.
Pathways
CD27 actively integrates into the NF-kB and mTOR signaling pathways which are important for regulating immune and inflammatory responses. Through these pathways CD27 works closely with proteins like TRAF2 and TRAF3 which are adapter molecules that transmit signals from the receptor. These signaling pathways provide a framework for understanding how CD27 and associated proteins work together to control immune cell behavior facilitating coordinated immune defense mechanisms.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Product promise
Associated Products
Alternative Product
Primary Antibodies
AB272072
Anti-CD27 antibody [BLR083G] - BSA free
primary-antibodies
cd27-antibody-blr083g-bsa-free-ab272072
0
(0 reviews)
Alternative Product
Primary Antibodies
AB265589
Anti-CD27 antibody [BLR083G]
primary-antibodies
cd27-antibody-blr083g-ab265589
0
(0 reviews)
Alternative Product
Primary Antibodies
AB131254
Anti-CD27 antibody [EPR8569]
primary-antibodies
cd27-antibody-epr8569-ab131254
5
(2 reviews)
Alternative Product
Primary Antibodies
AB133761
Anti-CD27 antibody [EPR8568]
primary-antibodies
cd27-antibody-epr8568-ab133761
0
(0 reviews)
Alternative Product
Primary Antibodies
AB192336
Anti-CD27 antibody [EPR8569] - Low endotoxin, Azide free
primary-antibodies
cd27-antibody-epr8569-low-endotoxin-azide-free-ab192336
5
(1 reviews)
Alternative Product
Primary Antibodies
AB192335
Anti-CD27 antibody [EPR8568] - BSA and Azide free
primary-antibodies
cd27-antibody-epr8568-bsa-and-azide-free-ab192335
0
(0 reviews)
Alternative Product
Primary Antibodies
AB214043
Anti-CD27 antibody [EPR20585]
primary-antibodies
cd27-antibody-epr20585-ab214043
5
(1 reviews)
Alternative Version
Primary Antibodies
AB268144
Anti-CD27 antibody [LPFS2/1611]
primary-antibodies
cd27-antibody-lpfs2-1611-ab268144
0
(0 reviews)
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com