AB233809

Anti-CD272/BTLA antibody [4B12A7]

Name: Anti-CD272/BTLA antibody [4B12A7] (ab233809) | Abcam
Brand: Abcam Limited
SKU: AB233809
Price: 495 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(1 Publication)

Mouse Monoclonal CD272/BTLA antibody. Suitable for Flow Cyt, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human BTLA aa 150-300.

View Alternative Names

CD272, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, BTLA

3 Images

Flow Cytometry - Anti-CD272/BTLA antibody [4B12A7] (AB233809)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD272/BTLA antibody [4B12A7] (AB233809)

Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling CD272/BTLA with ab233809 at 1/200 dilution (green) compared with a negative control (red).

Supplier Data

Western blot - Anti-CD272/BTLA antibody [4B12A7] (AB233809)

All lanes:

Western blot - Anti-CD272/BTLA antibody [4B12A7] (ab233809) at 1/500 dilution

Lane 1:

Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

CD272/BTLA (aa 179-289)-hIgG-Fc-transfected HEK-293 cell lysate

Predicted band size: 33 kDa

false

Western blot - Anti-CD272/BTLA antibody [4B12A7] (AB233809)

Expected recombinant MW is 38 kDa.

All lanes:

Western blot - Anti-CD272/BTLA antibody [4B12A7] (ab233809) at 1/500 dilution

All lanes:

Recombniant human CD272/BTLA (aa 179-289) protein

Predicted band size: 33 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4B12A7

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human BTLA aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q7Z6A9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD272 also known as BTLA (B and T Lymphocyte Attenuator) is an immunoregulatory protein with a mass approximately 34 kDa. This target is part of the CD28 immunoglobulin superfamily and is expressed on the surfaces of various immune cells including T cells B cells and dendritic cells. BTLA/CD272 serves as a co-inhibitory molecule important in maintaining immune homeostasis. Its expression is influenced by factors present in the microenvironment of immune tissues.

Biological function summary

BTLA functions as a negative regulator of immune responses by delivering inhibitory signals to immune cells upon binding to its ligand HVEM (Herpesvirus entry mediator). It forms a part of an immunological checkpoint complex that modulates lymphocyte activity to prevent overactivation and autoimmunity. This mechanism helps maintain a balance between immune defense and tolerance contributing to self-tolerance.

Pathways

The BTLA/CD272 protein engages with pathways involved in immune checkpoints and T-cell receptor signaling. It interacts with proteins like HVEM and collaborates with receptors such as PD-1 and CTLA-4. These interactions play a role in the downregulation of T-cell responses which is essential in various immune processes. The signaling pathways influenced by BTLA/CD272 are integral in moderating immune responses cooperation and adaptation.

BTLA is associated with autoimmune diseases and cancer. In autoimmune disorders an altered expression or function of BTLA can lead to dysregulated immune responses causing tissue damage. In cancer the protein may contribute to immune evasion by tumors aiding tumor growth and progression. The interplay of BTLA with proteins such as PD-1 makes it a potential target for immunotherapy strategies in oncology and autoimmunity treatments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed : 12796776, PubMed : 14652006, PubMed : 15568026, PubMed : 18193050). May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14 (PubMed : 19915044). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed : 19915044).

See full target information BTLA

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 82:2779-2796.e10 PubMed35675814

2022

AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay.

Applications

Unspecified application

Species

Unspecified reactive species

Hana Cho,Elizabeth T Abshire,Maximilian W Popp,Christoph Pröschel,Joshua L Schwartz,Gene W Yeo,Lynne E Maquat

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD272/BTLA antibody [4B12A7]

Flow Cytometry - Anti-CD272/BTLA antibody [4B12A7] (AB233809)

Western blot - Anti-CD272/BTLA antibody [4B12A7] (AB233809)

Western blot - Anti-CD272/BTLA antibody [4B12A7] (AB233809)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise