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Rabbit Recombinant Monoclonal CD272/BTLA antibody. Suitable for IHC-P, mIHC and reacts with Mouse samples.

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Images

Multiplex immunohistochemistry - Anti-CD272/BTLA antibody [EPR29068-115] (AB317827), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] (AB317827), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] (AB317827), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] (AB317827), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] (AB317827), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PmIHCWBIPFlow CytICC/IF
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Target data

Function

Inhibitory receptor on lymphocytes that negatively regulates antigen receptor signaling via PTPN6/SHP-1 and PTPN11/SHP-2 (PubMed:12796776, PubMed:14652006). May interact in cis (on the same cell) or in trans (on other cells) with TNFRSF14 (PubMed:19915044). In cis interactions, appears to play an immune regulatory role inhibiting in trans interactions in naive T cells to maintain a resting state. In trans interactions, can predominate during adaptive immune response to provide survival signals to effector T cells (PubMed:19915044).

Alternative names

CD272, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein, Btla

Recommended products

Rabbit Recombinant Monoclonal CD272/BTLA antibody. Suitable for IHC-P, mIHC and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29068-115
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CD272 also known as BTLA (B and T Lymphocyte Attenuator) is an immunoregulatory protein with a mass approximately 34 kDa. This target is part of the CD28 immunoglobulin superfamily and is expressed on the surfaces of various immune cells including T cells B cells and dendritic cells. BTLA/CD272 serves as a co-inhibitory molecule important in maintaining immune homeostasis. Its expression is influenced by factors present in the microenvironment of immune tissues.

Biological function summary

BTLA functions as a negative regulator of immune responses by delivering inhibitory signals to immune cells upon binding to its ligand HVEM (Herpesvirus entry mediator). It forms a part of an immunological checkpoint complex that modulates lymphocyte activity to prevent overactivation and autoimmunity. This mechanism helps maintain a balance between immune defense and tolerance contributing to self-tolerance.

Pathways

The BTLA/CD272 protein engages with pathways involved in immune checkpoints and T-cell receptor signaling. It interacts with proteins like HVEM and collaborates with receptors such as PD-1 and CTLA-4. These interactions play a role in the downregulation of T-cell responses which is essential in various immune processes. The signaling pathways influenced by BTLA/CD272 are integral in moderating immune responses cooperation and adaptation.

Associated diseases and disorders

BTLA is associated with autoimmune diseases and cancer. In autoimmune disorders an altered expression or function of BTLA can lead to dysregulated immune responses causing tissue damage. In cancer the protein may contribute to immune evasion by tumors aiding tumor growth and progression. The interplay of BTLA with proteins such as PD-1 makes it a potential target for immunotherapy strategies in oncology and autoimmunity treatments.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Multiplex immunohistochemistry - Anti-CD272/BTLA antibody [EPR29068-115] (ab317827), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD272/BTLA antibody [EPR29068-115] (ab317827)

    CD272/BTLA Multiplex immunohistochemistry staining of Mouse spleen using rabbit Anti-CD272/BTLA antibody

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spleen tissue staining CD272/BTLA with ab317827 at a 1:1000 (0.44 ug/ml) dilution, ab317827 anti-BTLA used at 1:100 (4.92 ug/ml) dilution and Anti-CD3 epsilon antibody [SP7] ab16669 anti-CD3 used at a 1:150 (0.06 ug/ml) dilution.

    Panel A: merged staining of anti-CD19 (magenta; Opal™690), anti-BTLA (green; Opal™520) and anti-CD3 (yellow; Opal™570) on mouse spleen.

    Panel B: anti-CD19 staining B lymphocytes in mouse spleen.

    Panel C: anti-BTLA staining B lymphocytes and T lymphocytes in mouse spleen.

    Panel D: anti-CD3 staining T lymphocytes in mouse spleen.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-CD19 antibody [EPR23174-145] ab245235, ab317827 and Anti-CD3 epsilon antibody [SP7] ab16669 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] (ab317827), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] (ab317827)

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling CD272/BTLA with ab317827 at 1/100 (4.92 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in mouse cardiac muscle.

    The section was incubated with ab317827 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] (ab317827), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] (ab317827)

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling CD272/BTLA with ab317827 at 1/100 (4.92 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: No staining in mouse cerebrum.

    The section was incubated with ab317827 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] (ab317827), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] (ab317827)

    Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling CD272/BTLA with ab317827 at 1/100 (4.92 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in immune cells of mouse lung.

    The section was incubated with ab317827 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] (ab317827), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD272/BTLA antibody [EPR29068-115] (ab317827)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD272/BTLA with ab317827 at 1/100 (4.92 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining in mouse spleen.

    The section was incubated with ab317827 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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