AB105922

Anti-CD276 antibody [6A1]

Name: Anti-CD276 antibody [6A1] (ab105922) | Abcam
Brand: Abcam Limited
SKU: AB105922
Rating: 4 (3 reviews)

(3 Reviews)

(15 Publications)

Mouse Monoclonal CD276 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CD276 aa 1-150.

View Alternative Names

CD276, B7H3, PSEC0249, UNQ309/PRO352, CD276 antigen, 4Ig-B7-H3, B7 homolog 3, Costimulatory molecule, B7-H3

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (AB105922)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (AB105922)

ab105922, at a 1/200 dilution, staining CD276 in paraffin-embedded Human ovarian cancer tissues.

IHC-P

AbReview58550****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (AB105922)

Formaldehyde-fixed, paraffin-embedded human tonsil tissue stained for CD276 using ab105922 at 1/800 dilution in immunohistochemical analysis.

This image is courtesy of an anonymous Abreview.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [6A1] (AB105922)

ab105922, at a 1/200 dilution, staining CD276 in paraffin-embedded Human cervical cancer tissues.

Unknown

Western blot - Anti-CD276 antibody [6A1] (AB105922)

All lanes:

Western blot - Anti-CD276 antibody [6A1] (ab105922) at 1/200 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

Transfected HEK293 cell lysate

Predicted band size: 57 kDa,83 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6A1

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CD276 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q5ZPR3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "" } } }

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Product details

This product was changed from ascites to supernatant. Lot no's high than GR209552-21 are from Tissue Culture Supernatant

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD276 also known as B7-H3 is an immunoregulatory protein with a mass of approximately 57 kDa although post-translational modifications can increase its observed size. It is a member of the B7 family which is part of the immunoglobulin superfamily. CD276 is widely expressed in various tissues including placenta liver heart and also in many tumor cells. It is observed in lesser amounts in normal tissues making it an interesting target for cancer research. Various assays like HEK293 and HEK293T cell confluency studies often use CD276 to explore its expression under different conditions and seeding densities which are represented in confluency charts.

Biological function summary

CD276 modulates immune responses by acting as a co-inhibitory ligand impacting T-cell proliferation and cytokine synthesis. Not part of a known complex it plays a role in the adaptive immune system by providing negative feedback regulation that can inhibit the immune response. This capability has implications in preventing autoimmunity but may also contribute to tumor immune evasion by reducing effective anti-tumor responses. The B7-H3/CD276 interaction with receptors such as IL receptor family members suggests its diverse immunomodulatory functions.

Pathways

CD276 participates in the T-cell receptor (TCR) signaling pathways influencing immune checkpoint pathways. It interacts with other immune checkpoint proteins like PD-L1 and CTLA-4 but its exact receptor partner remains unidentified. The presence of CD276 in these pathways highlights its role in controlling T-cell activation and tolerance. By modulating TCR signals and impacting downstream effects CD276 serves as a regulatory point that can balance immune activation and inhibition critical in immune-mediated conditions.

CD276 has strong associations with cancer particularly in solid tumors such as prostate and breast cancer. It is often overexpressed in malignant tissues contributing to immune evasion by tumors. Researchers propose it as a potential target for cancer immunotherapy due to this attribute. CD276's link to disorders like asthma has also been suggested where its interaction with proteins involved in inflammatory pathways such as IL response elements can exacerbate the condition. This implicates it as a dual-role protein in both disease progression and potential therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.

See full target information CD276

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 22:809 PubMed39217342

2024

AC099850.3 promotes HBV-HCC cell proliferation and invasion through regulating CD276: a novel strategy for sorafenib and immune checkpoint combination therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Aoxiao He,Zhihao Huang,Qian Feng,Shan Zhang,Fan Li,Dan Li,Hongcheng Lu,Jiakun Wang

Journal of gastrointestinal oncology 15:1035-1049 PubMed38989423

2024

B7-H3 enhances colorectal cancer progression by regulating HB-EGF via HIF-1α.

Applications

Unspecified application

Species

Unspecified reactive species

Chenrui Hu,Shengjia Wang,Jin Wang,Xiaokang Ruan,Linwei Wu,Zhe Zhang,Xuefeng Wang,Jianglei Zhang,Yonghao Liu,Yao Li,Xin Zhao

Frontiers in immunology 13:984172 PubMed36159808

2022

Novel immune scoring dynamic nomograms based on B7-H3, B7-H4, and HHLA2: Potential prediction in survival and immunotherapeutic efficacy for gallbladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Lv,Shukun Han,Baokang Wu,Zhiyun Liang,Yang Li,Yizhou Zhang,Qi Lang,Chongli Zhong,Lei Fu,Yang Yu,Feng Xu,Yu Tian

Reproductive biology and endocrinology : RB&E 19:114 PubMed34289871

2021

Mifepristone inhibited the expression of B7-H2, B7-H3, B7-H4 and PD-L2 in adenomyosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Qin,Wenjing Sun,Chong Wang,Mingjiang Li,Xingbo Zhao,Changzhong Li,Hui Zhang

Cancer science 112:3314-3323 PubMed34080242

2021

High membrane expression of CMTM6 in hepatocellular carcinoma is associated with tumor recurrence.

Applications

Unspecified application

Species

Unspecified reactive species

Ryo Muranushi,Kenichiro Araki,Takehiko Yokobori,Batbayar Chingunjav,Kouki Hoshino,Gantumur Dolgormaa,Kei Hagiwara,Takahiro Yamanaka,Norihiro Ishii,Mariko Tsukagoshi,Takamichi Igarashi,Akira Watanabe,Norio Kubo,Norifumi Harimoto,Yuki Shimoda,Rie Sano,Tetsunari Oyama,Hiroshi Saeki,Ken Shirabe

Frontiers in oncology 11:649980 PubMed34178637

2021

MAPK-RAP1A Signaling Enriched in Hepatocellular Carcinoma Is Associated With Favorable Tumor-Infiltrating Immune Cells and Clinical Prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hailin Li,Guangyu Han,Xing Li,Bowen Li,Bo Wu,Hongyuan Jin,Lingli Wu,Wei Wang

BMC urology 21:60 PubMed33845814

2021

Expression patterns of the immune checkpoint ligand CD276 in urothelial carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wilhelm K Aicher,M Korn,L Reitnauer,F B Maurer,J Hennenlotter,P C Black,T Todenhofer,J Bedke,A Stenzl

World neurosurgery 135:e12-e18 PubMed31629137

2019

Expression and Clinical Significance of Immune Checkpoint Regulator B7-H3 (CD276) in Human Meningioma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaojiao Deng,Mengyin Ma,Daijun Wang,Hongda Zhu,Lingyang Hua,Shuchen Sun,Hong Chen,Haixia Cheng,Zhi Rong Qian,Qing Xie,Tao Zhang,Ye Gong

Molecular medicine reports 19:4315-4325 PubMed30942404

2019

High expression of B7‑H2 or B7‑H3 is associated with poor prognosis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Youshi Zheng,Naishun Liao,Yuan Wu,Ju Gao,Zhenli Li,Wenwen Liu,Yingchao Wang,Ming Li,Xiaolou Li,Li Chen,Wenmin Zhang,Bixing Zhao

Oncology letters 17:4004-4010 PubMed30930997

2019

Prognostic impact of tumor-infiltrating CD276/Foxp3-positive lymphocytes and associated circulating cytokines in patients undergoing radical nephrectomy for localized renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kota Iida,Makito Miyake,Kenta Onishi,Shunta Hori,Yosuke Morizawa,Daisuke Gotoh,Yoshitaka Itami,Sayuri Onishi,Yasushi Nakai,Satoshi Anai,Nobumichi Tanaka,Kiyohide Fujimoto

View all publications

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Product promise

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