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Rabbit Recombinant Monoclonal CD276 antibody. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-CD276 antibody [SP206], prediluted (AB228178), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178), expandable thumbnail
  • Western blot - Anti-CD276 antibody [SP206], prediluted (AB228178), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (AB228178), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: Tris buffered saline, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Predicted
Predicted
Rat
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1

Notes

Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

Pre-diluted / ready to use.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

7 products for Alternative Product

2 products for Alternative Version

Target data

Function

May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.

Alternative names

B7H3, PSEC0249, UNQ309/PRO352, CD276, B7H3, PSEC0249, UNQ309/PRO352, CD276 antigen, 4Ig-B7-H3, B7 homolog 3, Costimulatory molecule, B7-H3

Recommended products

Rabbit Recombinant Monoclonal CD276 antibody. Suitable for WB, IHC-P and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP206

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CD276 also known as B7-H3 is an immunoregulatory protein with a mass of approximately 57 kDa although post-translational modifications can increase its observed size. It is a member of the B7 family which is part of the immunoglobulin superfamily. CD276 is widely expressed in various tissues including placenta liver heart and also in many tumor cells. It is observed in lesser amounts in normal tissues making it an interesting target for cancer research. Various assays like HEK293 and HEK293T cell confluency studies often use CD276 to explore its expression under different conditions and seeding densities which are represented in confluency charts.

Biological function summary

CD276 modulates immune responses by acting as a co-inhibitory ligand impacting T-cell proliferation and cytokine synthesis. Not part of a known complex it plays a role in the adaptive immune system by providing negative feedback regulation that can inhibit the immune response. This capability has implications in preventing autoimmunity but may also contribute to tumor immune evasion by reducing effective anti-tumor responses. The B7-H3/CD276 interaction with receptors such as IL receptor family members suggests its diverse immunomodulatory functions.

Pathways

CD276 participates in the T-cell receptor (TCR) signaling pathways influencing immune checkpoint pathways. It interacts with other immune checkpoint proteins like PD-L1 and CTLA-4 but its exact receptor partner remains unidentified. The presence of CD276 in these pathways highlights its role in controlling T-cell activation and tolerance. By modulating TCR signals and impacting downstream effects CD276 serves as a regulatory point that can balance immune activation and inhibition critical in immune-mediated conditions.

Associated diseases and disorders

CD276 has strong associations with cancer particularly in solid tumors such as prostate and breast cancer. It is often overexpressed in malignant tissues contributing to immune evasion by tumors. Researchers propose it as a potential target for cancer immunotherapy due to this attribute. CD276's link to disorders like asthma has also been suggested where its interaction with proteins involved in inflammatory pathways such as IL response elements can exacerbate the condition. This implicates it as a dual-role protein in both disease progression and potential therapeutic targeting.

Product promise

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12 product images

  • Western blot - Anti-CD276 antibody [SP206], prediluted (ab228178), expandable thumbnail

    Western blot - Anti-CD276 antibody [SP206], prediluted (ab228178)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-CD276 antibody [SP206] ab227670).

    Lanes 1- 4: Merged signal (red and green). Green - Anti-CD276 antibody [SP206] ab227670 observed at 90-110 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

    Anti-CD276 antibody [SP206] ab227670 was shown to react with CD276 antigen in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human CD276 knockout HeLa cell line ab261756 (knockout cell lysate Human CD276 knockout HeLa cell lysate ab257098) was used. Wild-type HeLa and CD276 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-CD276 antibody [SP206] ab227670 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) overnight at 4°C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    Lanes 1 - 4: Western blot - Anti-CD276 antibody [SP206], prediluted (ab228178) at 1/400 dilution

    Lanes 1 - 4: Western blot - Anti-CD276 antibody [SP206] (Anti-CD276 antibody [SP206] ab227670) at 1/400 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: CD276 knockout HeLa cell lysate at 20 µg

    Lane 3: LNCaP cell lysate at 20 µg

    Lane 4: Raji cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 57 kDa

    Observed band size: 90-110 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

    Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.

  • Western blot - Anti-CD276 antibody [SP206], prediluted (ab228178), expandable thumbnail

    Western blot - Anti-CD276 antibody [SP206], prediluted (ab228178)

    This data was developed using the same antibody clone in a different buffer formulation (Anti-CD276 antibody [SP206] ab227670).

    Lanes 1-4: Merged signal (red and green). Green - Anti-CD276 antibody [SP206] ab227670 observed at 90-110 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    Anti-CD276 antibody [SP206] ab227670 Anti-CD276 antibody [SP206] was shown to specifically react with CD276 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human CD276 knockout HEK-293T cell line ab266658 (knockout cell lysate Human CD276 knockout HEK-293T cell lysate ab257097) was used. Wild-type and CD276 knockout samples were subjected to SDS-PAGE. Anti-CD276 antibody [SP206] ab227670 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    Lanes 1 - 4: Western blot - Anti-CD276 antibody [SP206] (Anti-CD276 antibody [SP206] ab227670) at 1/1000 dilution

    Lanes 1 - 4: Western blot - Anti-CD276 antibody [SP206], prediluted (ab228178) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: CD276 knockout HEK293T cell lysate at 20 µg

    Lane 3: LNCaP cell lysate at 20 µg

    Lane 4: Raji cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 57 kDa

    Observed band size: 90-110 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

    Formalin-fixed, paraffin-embedded human stomach tissue stained for CD276 with ab228178 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

    Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

    Formalin-fixed, paraffin-embedded human colon tissue stained for CD276 with ab228178 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

    Formalin-fixed, paraffin-embedded human lung SCC tissue stained for CD276 with ab228178 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

    Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for CD276 with ab228178 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

    Formalin-fixed, paraffin-embedded human lung tissue stained for CD276 with ab228178 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

    Formalin-fixed, paraffin-embedded human testis tissue stained for CD276 with ab228178 in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP206], prediluted (ab228178)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD276 with ab228178 in immunohistochemical analysis.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com