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AB227679

Anti-CD276 antibody [SP265] - C-terminal

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(3 Publications)

Rabbit Recombinant Monoclonal CD276 antibody. C-terminal. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CD276, B7H3, PSEC0249, UNQ309/PRO352, CD276 antigen, 4Ig-B7-H3, B7 homolog 3, Costimulatory molecule, B7-H3

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-CD276 antibody [SP265] - C-terminal (AB227679)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD276 antibody [SP265] - C-terminal (AB227679)

Immunocytochemistry/ Immunofluorescence analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling CD276 with purified ab227679 at 1/25 (9.24 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488 ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow Cytometry - Anti-CD276 antibody [SP265] - C-terminal (AB227679)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD276 antibody [SP265] - C-terminal (AB227679)

Flow cytometric analysis of PC-3 (human prostate adenocarcinoma cell line) cell line labeling CC276 with ab227679 at 1/400 (green) compared with a rabbit isotype control (blue).

Immunocytochemistry/ Immunofluorescence - Anti-CD276 antibody [SP265] - C-terminal (AB227679)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD276 antibody [SP265] - C-terminal (AB227679)

ab227679 staining CD276 in wild-type HEK293 cells (top panel) and CD276 knockout HEK293 cells (ab266658) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab227679 at 1/100 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

Western blot - Anti-CD276 antibody [SP265] - C-terminal (AB227679)
  • WB

Supplier Data

Western blot - Anti-CD276 antibody [SP265] - C-terminal (AB227679)

All lanes:

Western blot - Anti-CD276 antibody [SP265] - C-terminal (ab227679) at 1/100 dilution

All lanes:

Hs 700T (human pancreatic adenocarcinoma cell line) cell lysate

Predicted band size: 57 kDa

true

  • Carrier free

    Anti-CD276 antibody [SP265] - BSA and Azide free

  • 578 PE

    PE Anti-CD276 antibody [SP265] (C-terminal)

  • 660 APC

    APC Anti-CD276 antibody [SP265] (C-terminal)

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD276 antibody [SP265] - C-terminal

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD276 antibody [SP265] - C-terminal

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-CD276 antibody [SP265] - C-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-CD276 antibody [SP265] - C-terminal

  • HRP

    HRP Anti-CD276 antibody [SP265] (C-terminal)

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-CD276 antibody [SP265] - C-terminal

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-CD276 antibody [SP265] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP265

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

AB209895

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Complementary Products

Assay Kits

AB221831

Human CD276 ELISA Kit

Recombinant

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/25 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD276 also known as B7-H3 is an immunoregulatory protein with a mass of approximately 57 kDa although post-translational modifications can increase its observed size. It is a member of the B7 family which is part of the immunoglobulin superfamily. CD276 is widely expressed in various tissues including placenta liver heart and also in many tumor cells. It is observed in lesser amounts in normal tissues making it an interesting target for cancer research. Various assays like HEK293 and HEK293T cell confluency studies often use CD276 to explore its expression under different conditions and seeding densities which are represented in confluency charts.
Biological function summary

CD276 modulates immune responses by acting as a co-inhibitory ligand impacting T-cell proliferation and cytokine synthesis. Not part of a known complex it plays a role in the adaptive immune system by providing negative feedback regulation that can inhibit the immune response. This capability has implications in preventing autoimmunity but may also contribute to tumor immune evasion by reducing effective anti-tumor responses. The B7-H3/CD276 interaction with receptors such as IL receptor family members suggests its diverse immunomodulatory functions.

Pathways

CD276 participates in the T-cell receptor (TCR) signaling pathways influencing immune checkpoint pathways. It interacts with other immune checkpoint proteins like PD-L1 and CTLA-4 but its exact receptor partner remains unidentified. The presence of CD276 in these pathways highlights its role in controlling T-cell activation and tolerance. By modulating TCR signals and impacting downstream effects CD276 serves as a regulatory point that can balance immune activation and inhibition critical in immune-mediated conditions.

CD276 has strong associations with cancer particularly in solid tumors such as prostate and breast cancer. It is often overexpressed in malignant tissues contributing to immune evasion by tumors. Researchers propose it as a potential target for cancer immunotherapy due to this attribute. CD276's link to disorders like asthma has also been suggested where its interaction with proteins involved in inflammatory pathways such as IL response elements can exacerbate the condition. This implicates it as a dual-role protein in both disease progression and potential therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.
See full target information CD276
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 44:115089 PubMed39709604

2024

Increased nuclear factor I-mediated chromatin access drives transition to androgen receptor splice variant dependence in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Larysa Poluben,Mannan Nouri,Jiaqian Liang,Shaoyong Chen,Andreas Varkaris,Betul Ersoy-Fazlioglu,Olga Voznesensky,Irene I Lee,Xintao Qiu,Laura Cato,Ji-Heui Seo,Matthew L Freedman,Adam G Sowalsky,Nathan A Lack,Eva Corey,Peter S Nelson,Myles Brown,Henry W Long,Joshua W Russo,Steven P Balk

Frontiers in oncology 10:1527 PubMed32984002

2020

MEK Inhibitor Augments Antitumor Activity of B7-H3-Redirected Bispecific Antibody.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjian Li,Cheng Huang,Zongliang Zhang,Yunyu Feng,Zeng Wang,Xin Tang,Kunhong Zhong,Yating Hu,Gang Guo,Liangxue Zhou,Wenhao Guo,Jianguo Xu,Hui Yang,Aiping Tong

Theranostics 10:10498-10512 PubMed32929362

2020

Combination therapy with B7H3-redirected bispecific antibody and Sorafenib elicits enhanced synergistic antitumor efficacy.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Huang,Hongjian Li,Yunyu Feng,Xiaoling Li,Zongliang Zhang,Caiying Jiang,Jichao Wang,Chenli Yang,Yuying Fu,Min Mu,Shasha Zhao,Zeng Wang,Yi Kuang,Huan Hou,Yuelong Wang,Wenhao Guo,Jianguo Xu,Hui Yang,Liangxue Zhou,Aiping Tong,Gang Guo
View all publications
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Product promise

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