AB203084

Anti-CD28 antibody

Name: Anti-CD28 antibody (ab203084) | Abcam
Brand: Abcam Limited
SKU: AB203084
Price: 510 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal CD28 antibody. Suitable for Flow Cyt, IHC-P and reacts with Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Mouse Cd28 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD28, T-cell-specific surface glycoprotein CD28, Cd28

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody (AB203084)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody (AB203084)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse lung tissue labeling CD28 with ab203084 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Flow Cytometry - Anti-CD28 antibody (AB203084)

Flow Cyt

Unknown

Flow Cytometry - Anti-CD28 antibody (AB203084)

Flow cytometric analysis of Mouse thymocytes labeling CD28 with ab203084 at 1/100 dilution for 30 minutes followed by incubation with a FITC conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody (AB203084)

Immunohistochemical analysis of formalin-fixed, paraffin embedded rat colitis tissue labeling CD28 with ab203084 at 1/200 dilution followed by Goat Anti-Rabbit IgG, Cy3 conjugated at 1/200 dilution for 40 minutes at 37°C.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD28 antibody (AB203084)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat colitis tissue labeling CD28 with ab203084 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

Flow Cyt, IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Cd28 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P31041

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/20 - 1/100", "FlowCyt-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "When using a fluorescent probe the recommended dilution is 1/50 – 1/200." }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "When using a fluorescent probe the recommended dilution is 1/50 – 1/200." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD28 also known as T-cell-specific surface glycoprotein CD28 or TP44 plays an important role as a co-stimulatory receptor on T cells. This glycoprotein has a molecular mass of about 44 kDa and is located mainly on the cell surface of T cells. CD28 expression also occurs on thymocytes and some B cells. It forms part of the immunoglobulin superfamily featuring in significant antibody interactions. Researchers often use anti-CD28 antibodies for various immunological assays including CD28 ELISA to study its function.

Biological function summary

CD28 co-stimulates T cells after antigen presentation. It enhances T cell proliferation survival and cytokine production. CD28 functions alongside the T cell receptor (TCR) complex mediating signals that promote full T cell activation. This interaction amplifies the immune response forming an important axis of immune system communication. CD28 interacts with its ligands B7-1 (CD80) and B7-2 (CD86) found on antigen-presenting cells (APCs) illustrating its role in immune system dynamics.

Pathways

CD28 is integral to the PI3K/Akt and MAPK/ERK signaling pathways. These pathways conduct signals critical for cellular growth and survival linking CD28 to downstream transcription factors. The interaction with another protein CTLA-4 acts as a regulatory balance for CD28 signaling preventing over-activation of T cells. This balance influences immune homeostasis and tolerance providing a framework for understanding costimulatory and inhibitory signaling.

CD28's role becomes evident in autoimmune diseases and transplant rejection. Abnormal CD28 signaling links to rheumatoid arthritis where inappropriate T cell activation occurs. Similarly CD28 activity contributes to organ transplant rejection by stimulating T cell-mediated responses against transplanted tissues. CTLA-4 through regulatory feedback can modify these effects providing therapeutic target strategies for managing such disorders. Efforts to modulate CD28 activity involve developing therapeutic agents such as anti-CD28 monoclonal antibodies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis (PubMed : 7688139). Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells.

See full target information Cd28

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1517968 PubMed39845968

2025

Prognostic value of immunosuppression scores in patients with esophageal squamous cell carcinoma: a multicenter study.

Applications

Unspecified application

Species

Unspecified reactive species

Shao-Jun Xu,Yun-Fan Luo,Jin Huang,Jia-Hua Tu,Chao Chen,Yan-Ming Shen,Zhao-Min Sun,Shu-Chen Chen

Mediators of inflammation 2022:1153300 PubMed36262548

2022

M2c Macrophages Protect Mice from Adriamycin-Induced Nephropathy by Upregulating CD62L in Tregs.

Applications

Unspecified application

Species

Unspecified reactive species

Junyu Lu,Shengqiu Lv,Jielong Pang,Tao Qin,Yegui Yang,Weisheng Lu,Zhengzhao Li,Geng Yang,Jianfeng Zhang

Journal of neuroinflammation 18:31 PubMed33482848

2021

Chronic exposure to IL-6 induces a desensitized phenotype of the microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Mireia Recasens,Beatriz Almolda,Jeús Pérez-Clausell,Iain L Campbell,Berta González,Bernardo Castellano

Cell biology international 43:1286-1295 PubMed30912221

2019

Immunohistochemical biomarkers and distribution of telocytes in ApoE mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xu,Hu Tian,Jialin Cheng,Shubin Liang,Teng Li,Ju Liu

View all publications

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