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AB193350

Anti-CD28 antibody [CB28]

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(2 Publications)

Mouse Monoclonal CD28 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CD28.

View Alternative Names

CD28, T-cell-specific surface glycoprotein CD28, TP44

2 Images
Flow Cytometry - Anti-CD28 antibody [CB28] (AB193350)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD28 antibody [CB28] (AB193350)

Flow Cytometric Analysis of paraformaldehyde-fixed Jurkat cells using ab193350 followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Immunocytochemistry/ Immunofluorescence - Anti-CD28 antibody [CB28] (AB193350)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD28 antibody [CB28] (AB193350)

Immunofluorescent staining of Jurkat cells using ab193350 followed by goat anti-Mouse IgG conjugated to CF488 (green). Red Dot is used to label nuclei (red).

  • 578 PE

    PE Anti-CD28 antibody [CB28]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CB28

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CD28.

P10747

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification notes
ab193350 was purified from Bioreactor Concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD28 also known as T-cell-specific surface glycoprotein CD28 or TP44 plays an important role as a co-stimulatory receptor on T cells. This glycoprotein has a molecular mass of about 44 kDa and is located mainly on the cell surface of T cells. CD28 expression also occurs on thymocytes and some B cells. It forms part of the immunoglobulin superfamily featuring in significant antibody interactions. Researchers often use anti-CD28 antibodies for various immunological assays including CD28 ELISA to study its function.
Biological function summary

CD28 co-stimulates T cells after antigen presentation. It enhances T cell proliferation survival and cytokine production. CD28 functions alongside the T cell receptor (TCR) complex mediating signals that promote full T cell activation. This interaction amplifies the immune response forming an important axis of immune system communication. CD28 interacts with its ligands B7-1 (CD80) and B7-2 (CD86) found on antigen-presenting cells (APCs) illustrating its role in immune system dynamics.

Pathways

CD28 is integral to the PI3K/Akt and MAPK/ERK signaling pathways. These pathways conduct signals critical for cellular growth and survival linking CD28 to downstream transcription factors. The interaction with another protein CTLA-4 acts as a regulatory balance for CD28 signaling preventing over-activation of T cells. This balance influences immune homeostasis and tolerance providing a framework for understanding costimulatory and inhibitory signaling.

CD28's role becomes evident in autoimmune diseases and transplant rejection. Abnormal CD28 signaling links to rheumatoid arthritis where inappropriate T cell activation occurs. Similarly CD28 activity contributes to organ transplant rejection by stimulating T cell-mediated responses against transplanted tissues. CTLA-4 through regulatory feedback can modify these effects providing therapeutic target strategies for managing such disorders. Efforts to modulate CD28 activity involve developing therapeutic agents such as anti-CD28 monoclonal antibodies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis (PubMed : 1650475, PubMed : 7568038). Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells (PubMed : 24665965). Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets (PubMed : 12196291, PubMed : 1650475, PubMed : 35397202). Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways (PubMed : 21964608, PubMed : 24665965, PubMed : 7568038). In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells (PubMed : 8617933).. Isoform 3. Enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed : 15067037).
See full target information CD28
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 119:105884 PubMed40803203

2025

Tumour extracellular vesicle surface Protein-mRNA integration assay for early detection of epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Tzu Yen,Chen Zhao,Tian Gao,Jacqueline Ziqian Yang,Ning Kang,Jiahui Pu,Lei Qiu,Qixin Hu,Hyoyong Kim,Anmin Wang,Junseok Lee,Ryan Y Zhang,Na Liu,Yue Ma,You-Ren Ji,Yong Ju,Lynn L Zheng,James Lee-South,Vivian X Zuo,Audrey Qian,Aaron Kwan,Yating Zhang,Shenghua Zhang,Zhili Wang,Jing Ren,Huaichao Liu,Zihan Wang,Yang Yue,Jina Kim,Jennifer Sun,Gabriella A DiBernardo,Laura B James-Allan,Ying Chen,Weipei Zhu,Guoyun Wang,Renjun Pei,Sanaz Memarzadeh,Sungyong You,Bobbie J Rimel,Kate Lawrenson,Beth Y Karlan,Myung Shin Sim,Shaohua Lu,Jipeng Wan,Na Sun,Hsian-Rong Tseng,Yazhen Zhu

British journal of cancer 130:1599-1608 PubMed38519706

2024

Persistence of peripheral CD8 + CD28- T cells indicates a favourable outcome and tumour immunity in first-line HER2-positive metastatic breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoran Liu,Xiangming Cheng,Feng Xie,Kun Li,Yongcan Shi,Bin Shao,Xu Liang,Fengling Wan,Shidong Jia,Yue Zhang,Yiqiang Liu,Huiping Li
View all publications
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Product promise

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