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AB25234

Anti-CD28 antibody [PV-1]

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(7 Publications)

Armenian hamster Monoclonal CD28 antibody. Suitable for IP, Flow Cyt, FuncS (Act) and reacts with Mouse samples. Cited in 7 publications.

View Alternative Names

CD28, T-cell-specific surface glycoprotein CD28, Cd28

  • 519 FITC

    FITC Anti-CD28 antibody [PV-1]

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

PV-1

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

FuncS (Act), Flow Cyt, IP

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FuncSAct" : {"fullname" : "Functional Studies (Act)", "shortname":"FuncS (Act)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>In vitro costimulation of T cells and NK cells. <a href='/en-us/products/primary-antibodies/armenian-hamster-igg-monoclonal-htk888-isotype-control-ab18479'>ab18479</a> - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "FuncSAct-species-checked": "guaranteed", "FuncSAct-species-dilution-info": "", "FuncSAct-species-notes": "<p>In vitro costimulation of T cells and NK cells.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 8.2 Constituents: 100% Borate buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD28 also known as T-cell-specific surface glycoprotein CD28 or TP44 plays an important role as a co-stimulatory receptor on T cells. This glycoprotein has a molecular mass of about 44 kDa and is located mainly on the cell surface of T cells. CD28 expression also occurs on thymocytes and some B cells. It forms part of the immunoglobulin superfamily featuring in significant antibody interactions. Researchers often use anti-CD28 antibodies for various immunological assays including CD28 ELISA to study its function.
Biological function summary

CD28 co-stimulates T cells after antigen presentation. It enhances T cell proliferation survival and cytokine production. CD28 functions alongside the T cell receptor (TCR) complex mediating signals that promote full T cell activation. This interaction amplifies the immune response forming an important axis of immune system communication. CD28 interacts with its ligands B7-1 (CD80) and B7-2 (CD86) found on antigen-presenting cells (APCs) illustrating its role in immune system dynamics.

Pathways

CD28 is integral to the PI3K/Akt and MAPK/ERK signaling pathways. These pathways conduct signals critical for cellular growth and survival linking CD28 to downstream transcription factors. The interaction with another protein CTLA-4 acts as a regulatory balance for CD28 signaling preventing over-activation of T cells. This balance influences immune homeostasis and tolerance providing a framework for understanding costimulatory and inhibitory signaling.

CD28's role becomes evident in autoimmune diseases and transplant rejection. Abnormal CD28 signaling links to rheumatoid arthritis where inappropriate T cell activation occurs. Similarly CD28 activity contributes to organ transplant rejection by stimulating T cell-mediated responses against transplanted tissues. CTLA-4 through regulatory feedback can modify these effects providing therapeutic target strategies for managing such disorders. Efforts to modulate CD28 activity involve developing therapeutic agents such as anti-CD28 monoclonal antibodies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor that plays a role in T-cell activation, proliferation, survival and the maintenance of immune homeostasis (PubMed : 7688139). Functions not only as an amplifier of TCR signals but delivers unique signals that control intracellular biochemical events that alter the gene expression program of T-cells. Stimulation upon engagement of its cognate ligands CD80 or CD86 increases proliferation and expression of various cytokines in particular IL2 production in both CD4(+) and CD8(+) T-cell subsets. Mechanistically, ligation induces recruitment of protein kinase C-theta/PRKCQ and GRB2 leading to NF-kappa-B activation via both PI3K/Akt-dependent and -independent pathways. In conjunction with TCR/CD3 ligation and CD40L costimulation, enhances the production of IL4 and IL10 in T-cells.
See full target information Cd28
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Neural regeneration research 17:2765-2770 PubMed35662227

2022

NR4A1 agonist cytosporone B attenuates neuroinflammation in a mouse model of multiple sclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Zhen Yu,Bing-Qing Zhu,Lin Zhu,Shuo Li,Li-Mei Wang

Cell reports 31:107748 PubMed32521273

2020

Chromatin Priming Renders T Cell Tolerance-Associated Genes Sensitive to Activation below the Signaling Threshold for Immune Response Genes.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah L Bevington,Sky T H Ng,Graham J Britton,Peter Keane,David C Wraith,Peter N Cockerill

Frontiers in immunology 9:3157 PubMed30693001

2019

PDL1 Fusion Protein Protects Against Experimental Cerebral Malaria via Repressing Over-Reactive CD8 T Cell Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wang,Yue Li,Yan Shen,Jiao Liang,Yinghui Li,Yuxiao Huang,Xuewu Liu,Dongbo Jiang,Shuya Yang,Ya Zhao,Kun Yang

PloS one 11:e0164099 PubMed27711247

2016

Predicting the Response to Intravenous Immunoglobulins in an Animal Model of Chronic Neuritis.

Applications

Flow Cyt

Species

Unspecified reactive species

Gerd Meyer Zu Horste,Steffen Cordes,Johannes Pfaff,Christian Mathys,Anne K Mausberg,Martin Bendszus,Mirko Pham,Hans-Peter Hartung,Bernd C Kieseier

Nature medicine 21:638-46 PubMed26005855

2015

Metabolic control of type 1 regulatory T cell differentiation by AHR and HIF1-α.

Applications

Unspecified application

Species

Unspecified reactive species

Ivan D Mascanfroni,Maisa C Takenaka,Ada Yeste,Bonny Patel,Yan Wu,Jessica E Kenison,Shafiuddin Siddiqui,Alexandre S Basso,Leo E Otterbein,Drew M Pardoll,Fan Pan,Avner Priel,Clary B Clish,Simon C Robson,Francisco J Quintana

Nature communications 6:6113 PubMed25672245

2015

Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolas Fischer,Greg Elson,Giovanni Magistrelli,Elie Dheilly,Nicolas Fouque,Amélie Laurendon,Franck Gueneau,Ulla Ravn,Jean-François Depoisier,Valery Moine,Sylvain Raimondi,Pauline Malinge,Laura Di Grazia,François Rousseau,Yves Poitevin,Sébastien Calloud,Pierre-Alexis Cayatte,Mathias Alcoz,Guillemette Pontini,Séverine Fagète,Lucile Broyer,Marie Corbier,Delphine Schrag,Gérard Didelot,Nicolas Bosson,Nessie Costes,Laura Cons,Vanessa Buatois,Zoe Johnson,Walter Ferlin,Krzysztof Masternak,Marie Kosco-Vilbois

Nature immunology 13:770-7 PubMed22751139

2012

Aiolos promotes TH17 differentiation by directly silencing Il2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Francisco J Quintana,Hulin Jin,Evan J Burns,Meghan Nadeau,Ada Yeste,Deepak Kumar,Manu Rangachari,Chen Zhu,Sheng Xiao,John Seavitt,Katia Georgopoulos,Vijay K Kuchroo
View all publications
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