Rabbit Polyclonal CD3d antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 12 publications.
Images
Publications
Filter by
- Cells 9:2020IL-13 as Target to Reduce Cholestasis and Dysbiosis in Knockout Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuisa Hahn et. al.PubMed 32846954
- International journal of molecular sciences 19:2018Topical Plant Polyphenols Prevent Type I Interferon Signaling in the Skin and Suppress Contact Hypersensitivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Luigia Carbone et. al.PubMed 30200670
- Molecular medicine reports 17:7683-76912018Calotropin regulates the apoptosis of non‑small cell cancer by regulating the cytotoxic T‑lymphocyte associated antigen 4‑mediated TGF‑β/ERK signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLu Tian et. al.PubMed 29620207
- Breast cancer research : BCR 19:902017Inhibition of basal-like breast cancer growth by FTY720 in combination with epidermal growth factor receptor kinase blockade.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJanet L Martin,Sohel M Julovi,Mike Z Lin,Hasanthi C de Silva,Frances M Boyle,Robert C BaxterPubMed 28778177
- The Journal of clinical investigation 127:843-8562017Regional astrocyte IFN signaling restricts pathogenesis during neurotropic viral infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrian P Daniels et. al.PubMed 28134626
- BMC complementary and alternative medicine 16:3342016Epigallocatechin-3-gallate (EGCG) inhibits imiquimod-induced psoriasis-like inflammation of BALB/c mice.
- Gene therapy 22:893-9002015Inhibition of pathological brain angiogenesis through systemic delivery of AAV vector expressing soluble FLT1.
- Cancer cell 21:668-6792012ATM phosphorylation of Mdm2 Ser394 regulates the amplitude and duration of the DNA damage response in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHugh S Gannon,Bruce A Woda,Stephen N JonesPubMed 22624716
- Arthritis research & therapy 12:R1882010CXC chemokine receptor 4 expressed in T cells plays an important role in the development of collagen-induced arthritis.Applications:IHC-FrReactive species:MouseSoo-Hyun Chung,Keisuke Seki,Byung-Il Choi,Keiko B Kimura,Akihiko Ito,Noriyuki Fujikado,Shinobu Saijo,Yoichiro IwakuraPubMed 20939892
- Experimental neurology 222:153-602010Deficiency of the negative immune regulator B7-H1 enhances inflammation and neuropathic pain after chronic constriction injury of mouse sciatic nerve.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Consider this alternative
Reactivity data
Select an application| IP | IHC | WB | |
|---|---|---|---|
| Human | Tested | Not recommended | Tested |
| Mouse | Expected | Not recommended | Tested |
| Rat | Expected | Not recommended | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.5 µg/mL | Notes - |
Species Rat | Dilution info 0.5 µg/mL | Notes - |
Species Human | Dilution info 0.5 µg/mL | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed:12215456).
Alternative names
CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain
Recommended products
Rabbit Polyclonal CD3d antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 12 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-CD3 antibody (ab16044)
Lanes 1 - 6: Merged signal (red and green). Green – ab16044 observed at 23 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab16044 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.
All lanes: Western blot - Anti-CD3 antibody (ab16044) at 1 µg/mL
Lane 1: THP1 whole cell lysate (-ve control) at 15 µg
Lane 2: Raji whole cell lysate (-ve control) at 15 µg
Lane 3: Jurkat whole cell lysate at 15 µg
Lane 4: Human Thymus tissue lysate at 15 µg
Lane 5: Mouse Thymus tissue lysate at 15 µg
Lane 6: Rat Thymus tissue lysate at 15 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 17 kDa, 23 kDa, 68 kDa
Western blot - Anti-CD3 antibody (ab16044)
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab16044 overnight at 4°C. Antibody binding was detected using VHH Single Domain Anti-Rabbit IgG Fc (HRP), and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.
All lanes: Western blot - Anti-CD3 antibody (ab16044) at 1 µg/mL
Lane 1: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 2: Thymus (Mouse) Tissue Lysate at 20 µg
Lane 3: Thymus (Rat) Tissue Lysate at 20 µg
SecondaryAll lanes: Western blot - VHH Single Domain Anti-Rabbit IgG Fc (HRP) (VHH Single Domain Anti-Rabbit IgG Fc (HRP) ab191866) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 18 kDa, 23 kDa, 48 kDa, 62 kDa
Exposure time: 8min
Immunoprecipitation - Anti-CD3 antibody (ab16044)
CD3 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to CD3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16044.
Secondary: Clean-Blot IP Detection Reagent (HRP) at 1/500 dilution.
Band: 23kDa; CD3All lanes: Immunoprecipitation - Anti-CD3 antibody (ab16044)
Predicted band size: 19 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com