Anti-CD3 antibody

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CD3 antibody (AB828)

This immunoperoxidase picture shows inflammatory cell infiltration with many T-lymphocytes in the interstitium of human kidneys with congenital nephrotic syndrome of the Finnish type (CNF, NPHS1). Immunoperoxidase staining of frozen sections with ab828 antibody (oval shaped, unstained spaces on the left are glomeruli).
This image was kindly supplied as part of the review submitted by Arvi-Matti-Kuusniemi.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (AB828)

ab828 positively staining formaldehyde fixed human tonsil tissue at 1/50 in conjunction with goat anti rabbit HRP. Detection by DAB.

Pressure cooker heat mediated antigen retrieval was employed in citrate buffer pH 6.0.

This image is courtesy of an Abreview submitted by Melanie Black on 22 Aug 05. We do not have any further information relating to this image.

• IHC-P

AbReview31990****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (AB828)

IHC-P image of CD3 staining on rat small intestine using ab828 (1 : 50). The section was deparafinized, rehydrated and subjected to heat mediated antigen retrival using citric acid at pH6. The section was then blocked using 1% BSA at 21°C for 10 mins. The primary antibody was used at 21°C for 2 hours. Goat polyclonal to Rabbit IgG conjugated to biotin was used as secondary antibody (1 : 250).

This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

• IHC-P

AbReview31996****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (AB828)

IHC-P image of CD3 staining on dog spleen using ab828 (1 : 50). The section was deparafinized, rehydrated and subjected to heat mediated antigen retrival using citric acid at pH6. The section was then blocked using 1% BSA at 21°C for 10 mins. The primary antibody was used at 21°C for 2 hours. Goat polyclonal to Rabbit IgG conjugated to biotin was used as secondary antibody (1 : 250).

This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

• IHC-P

AbReview31995****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (AB828)

IHC-P image of CD3 staining on monkey colon using ab828 (1 : 50). The section was deparafinized, rehydrated and subjected to heat mediated antigen retrival using citric acid at pH6. The section was then blocked using 1% BSA at 21°C for 10 mins. The primary antibody was used at 21°C for 2 hours. Goat polyclonal to Rabbit IgG conjugated to biotin was used as secondary antibody (1 : 250).

This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom