Rabbit Polyclonal CD3d antibody. Suitable for IHC-P, IHC-Fr and reacts with Human samples. Cited in 48 publications. Immunogen corresponding to Synthetic Peptide within Human CD3D conjugated to Bovine Serum Albumin.
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Publications
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- Scientific reports 13:86652023The potential role of the pseudobranch of molly fish (Poecilia sphenops) in immunity and cell regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDoaa M Mokhtar,Ramy K A Sayed,Giacomo Zaccone,Alessio Alesci,Marwa M HusseinPubMed 37248336
- Cells 11:2022Ependymal and Neural Stem Cells of Adult Molly Fish (, Valenciennes, 1846) Brain: Histomorphometry, Immunohistochemical, and Ultrastructural Studies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDoaa M Mokhtar,Ramy K A Sayed,Giacomo Zaccone,Marco Albano,Manal T HusseinPubMed 36078068
- Cancers 14:2022Validation of CD98hc as a Therapeutic Target for a Combination of Radiation and Immunotherapies in Head and Neck Squamous Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAyşe Sedef Köseer,Liliana R Loureiro,Justyna Jureczek,Nicola Mitwasi,Karla Elizabeth González Soto,Julia Aepler,Tabea Bartsch,Anja Feldmann,Leoni A Kunz-Schughart,Annett Linge,Mechthild Krause,Michael Bachmann,Claudia Arndt,Anna DubrovskaPubMed 35406454
- Xenotransplantation 28:e127042021Semi-automated digital quantification of cellular infiltrates for in vivo evaluation of transplanted islets of Langerhans encapsulated with bioactive materials.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatias Ramirez,Guillaume Courtoy,Oumaima Kharrat,Michele de Beukelaer,Nizar Mourad,Yves Guiot,Caroline Bouzin,Pierre GianelloPubMed 34218466
- Cells 10:2021Establishment of Human Leukocyte Antigen-Mismatched Immune Responses after Transplantation of Human Liver Bud in Humanized Mouse Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkihiro Mori,Soichiro Murata,Nao Tashiro,Tomomi Tadokoro,Satoshi Okamoto,Ryo Otsuka,Haruka Wada,Tomoki Murata,Takeshi Takahashi,Ken-Ichiro Seino,Hideki TaniguchiPubMed 33672150
- Pharmaceutics 12:2020Efficacy of Local Minocycline Agents in Treating Peri-Implantitis: An Experimental In Vivo Study in Beagle Dogs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSung-Wook Yoon,Myong-Ji Kim,Kyeong-Won Paeng,Kyeong Ae Yu,Chong-Kil Lee,Young Woo Song,Jae-Kook Cha,Ui-Won JungPubMed 33114168
- Microscopy research and technique 84:537-5472020The structural and ultrastructural organization of the cellular constituents of the trunk kidney of grass carp (Ctenopharyngodon idella).Applications:Unspecified applicationReactive species:Unspecified reactive speciesDoaa M MokhtarPubMed 32986903
- Journal of neuroscience research 98:2468-24952020Polyethylene glycol-fusion repair of sciatic allografts in female rats achieves immunotolerance via attenuated innate and adaptive responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTyler A Smith,Cameron L Ghergherehchi,Michelle Mikesh,Jaimie T Shores,Haley O Tucker,George D BittnerPubMed 32931034
- Pharmaceutics 12:2020Locally Applied Slow-Release of Minocycline Microspheres in the Treatment of Peri-Implant Mucositis: An Experimental In Vivo Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSung-Wook Yoon,Myong-Ji Kim,Kyeong-Won Paeng,Kyeong Ae Yu,Chong-Kil Lee,Young Woo Song,Jae-Kook Cha,Mariano Sanz,Ui-Won JungPubMed 32708741
- Frontiers in immunology 10:31062020Immune Cell Infiltrate in Chronic-Active Antibody-Mediated Rejection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKasia A Sablik,Ekaterina S Jordanova,Noelle Pocorni,Marian C Clahsen-van Groningen,Michiel G H BetjesPubMed 32117198
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3 - 7.5
Preservative: 0.05% Sodium azide
Constituents: 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human CD3D conjugated to Bovine Serum Albumin. The exact immunogen used to generate this antibody is proprietary information. Database link P04234
Reactivity data
Select an application| IHC-P | IHC-Fr | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10.00000 - 1/50.00000 | Notes ABC method. |
Associated Products
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7 products for Alternative Product
Target data
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed:12215456).
Alternative names
CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain
Recommended products
Rabbit Polyclonal CD3d antibody. Suitable for IHC-P, IHC-Fr and reacts with Human samples. Cited in 48 publications. Immunogen corresponding to Synthetic Peptide within Human CD3D conjugated to Bovine Serum Albumin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3 - 7.5
Preservative: 0.05% Sodium azide
Constituents: 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human CD3D conjugated to Bovine Serum Albumin. The exact immunogen used to generate this antibody is proprietary information. Database link P04234
- Purification technique
- Affinity purification Protein A
- Specificity
ab828 is predicted to react with T cell areas in lymphoid tissues derived from various species: human, bovine, porcine, sheep, cat, dog, rat, mouse, Psammomys (sand rat), and chicken. The molecular weight of the polypeptide recognized by the antibody in bovine and swine extracts is 20 kDa.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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5 product images
Immunohistochemistry (Frozen sections) - Anti-CD3 antibody (ab828)
This immunoperoxidase picture shows inflammatory cell infiltration with many T-lymphocytes in the interstitium of human kidneys with congenital nephrotic syndrome of the Finnish type (CNF, NPHS1). Immunoperoxidase staining of frozen sections with ab828 antibody (oval shaped, unstained spaces on the left are glomeruli).
This image was kindly supplied as part of the review submitted by Arvi-Matti-Kuusniemi.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (ab828)
ab828 positively staining formaldehyde fixed human tonsil tissue at 1/50 in conjunction with goat anti rabbit HRP. Detection by DAB.
Pressure cooker heat mediated antigen retrieval was employed in citrate buffer pH 6.0.
This image is courtesy of an Abreview submitted by Melanie Black on 22 Aug 05. We do not have any further information relating to this image.
This image is courtesy of a customer review submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (ab828)
IHC-P image of CD3 staining on rat small intestine using ab828 (1:50). The section was deparafinized, rehydrated and subjected to heat mediated antigen retrival using citric acid at pH6. The section was then blocked using 1% BSA at 21°C for 10 mins. The primary antibody was used at 21°C for 2 hours. Goat polyclonal to Rabbit IgG conjugated to biotin was used as secondary antibody (1:250).
This image is courtesy of a customer review submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (ab828)
IHC-P image of CD3 staining on monkey colon using ab828 (1:50). The section was deparafinized, rehydrated and subjected to heat mediated antigen retrival using citric acid at pH6. The section was then blocked using 1% BSA at 21°C for 10 mins. The primary antibody was used at 21°C for 2 hours. Goat polyclonal to Rabbit IgG conjugated to biotin was used as secondary antibody (1:250).
This image is courtesy of a customer review submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody (ab828)
IHC-P image of CD3 staining on dog spleen using ab828 (1:50). The section was deparafinized, rehydrated and subjected to heat mediated antigen retrival using citric acid at pH6. The section was then blocked using 1% BSA at 21°C for 10 mins. The primary antibody was used at 21°C for 2 hours. Goat polyclonal to Rabbit IgG conjugated to biotin was used as secondary antibody (1:250).
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