Anti-CD3 antibody CD3-12 is a rat monoclonal antibody that is used in CD3 Western blot, IHC, Flow cytometry. Suitable for Human, Mouse, Rat samples.
- Recombinant format ensures batch-to-batch consistency
- Trusted by researchers since 2004
- Cited in over 100 publications
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (AB11089), expandable thumbnail](https://content.abcam.com/products/images/cd3-antibody-cd3-12-ab11089--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441544.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (AB11089)")
![Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (AB11089), expandable thumbnail](https://content.abcam.com/products/images/cd3-antibody-cd3-12-ab11089--flow-cytometry-intracellular-img441542.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (AB11089)")
![Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (AB11089), expandable thumbnail](https://content.abcam.com/products/images/cd3-antibody-cd3-12-ab11089--flow-cytometry-intracellular-img441543.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (AB11089)")
![Western blot - Anti-CD3 antibody [CD3-12] (AB11089), expandable thumbnail](https://content.abcam.com/products/images/cd3-antibody-cd3-12-ab11089--western-blot-img441545.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD3 antibody [CD3-12] (AB11089)")
Publications
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- The Journal of pharmacology and experimental therapeutics 388:12-222024Blockade of Proteinase-Activated Receptor 2 (PAR2) Attenuates Neuroinflammation in Experimental Autoimmune Encephalomyelitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRahil Eftekhari,Benjamin W Ewanchuk,Khalil S Rawji,Robin M Yates,Farshid Noorbakhsh,Hedwich F Kuipers,Morley D HollenbergPubMed 37699708
- NPJ precision oncology 7:1352023Imaging and AI based chromatin biomarkers for diagnosis and therapy evaluation from liquid biopsies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKiran Challa,Daniel Paysan,Dominic Leiser,Nadia Sauder,Damien C Weber,G V ShivashankarPubMed 38092866
- Molecular neurobiology 61:3105-31202023Beneficial Effect of Fingolimod in a Lafora Disease Mouse Model by Preventing Reactive Astrogliosis-Derived Neuroinflammation and Brain Infiltration of T-lymphocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTeresa Rubio,Ángela Campos-Rodríguez,Pascual SanzPubMed 37971656
- Histology and histopathology 39:623-6322023mitigates autoimmune hepatitis by regulating IL-33-induced Treg/Th17 imbalance via the TLR2/4 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianguo Song,Juan Dai,Xueping Chen,Fei Ding,Yanbo Ding,Liang Ma,Liwen ZhangPubMed 37916940
- Frontiers in immunology 14:12457082023Novel miRNA-based drug CD5-2 reduces liver tumor growth in diethylnitrosamine-treated mice by normalizing tumor vasculature and altering immune infiltrate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKen Liu,Jinbiao Chen,Yang Zhao,Jade Boland,Ka Ka Ting,Glen Lockwood,Catriona McKenzie,James Kench,Mathew A Vadas,Jennifer R Gamble,Geoffrey W McCaughanPubMed 37795103
- JID innovations : skin science from molecules to population health 3:1002212023Topical Calcipotriol Plus Imiquimod Immunotherapy for Nonkeratinocyte Skin Cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarjan Azin,Kenneth H Ngo,Jennet Hojanazarova,Shadmehr DemehriPubMed 37731472
- JCI insight 8:2023The MIF promoter SNP rs755622 is associated with immune activation in glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTyler J Alban,Matthew M Grabowski,Balint Otvos,Defne Bayik,Wesley Wang,Ajay Zalavadia,Vlad Makarov,Katie Troike,Mary McGraw,Anja Rabljenovic,Adam Lauko,Chase Neumann,Gustavo Roversi,Kristin A Waite,Gino Cioffi,Nirav Patil,Thuy T Tran,Kathleen McCortney,Alicia Steffens,C Marcela Diaz,J Mark Brown,Kathleen M Egan,Craig M Horbinski,Jill S Barnholtz-Sloan,Prajwal Rajappa,Michael A Vogelbaum,Richard Bucala,Timothy A Chan,Manmeet S Ahluwalia,Justin D LathiaPubMed 37252795
- JCI insight 8:2023Perturbation of endoplasmic reticulum proteostasis triggers tissue injury in the thyroid gland.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaohan Zhang,Crystal Young,Xiao-Hui Liao,Samuel Refetoff,Mauricio Torres,Yaron Tomer,Mihaela Stefan-Lifshitz,Hao Zhang,Dennis Larkin,Deyu Fang,Ling Qi,Peter ArvanPubMed 37345654
- Nature communications 14:12132023Fibroblastic reticular cells in lymph node potentiate white adipose tissue beiging through neuro-immune crosstalk in male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLai Yee Cheong,Baile Wang,Qin Wang,Leigang Jin,Kelvin H M Kwok,Xiaoping Wu,Lingling Shu,Huige Lin,Sookja Kim Chung,Kenneth K Y Cheng,Ruby L C Hoo,Aimin XuPubMed 36869026
- EBioMedicine 89:1044652023Selective emergence of antibody-secreting cells in the multiple sclerosis brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaurens Bogers,Hendrik J Engelenburg,Malou Janssen,Peter-Paul A Unger,Marie-José Melief,Annet F Wierenga-Wolf,Cheng-Chih Hsiao,Matthew R J Mason,Jörg Hamann,Jamie van Langelaar,Joost Smolders,Marvin M van LuijnPubMed 36796230
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human CD3E. The exact immunogen used to generate this antibody is proprietary information. Database link P07766
Reactivity data
Select an application| IHC-P | WB | Flow Cyt | |
|---|---|---|---|
| Human | Not recommended | Expected | Tested |
| Mouse | Tested | Expected | Tested |
| Rat | Expected | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes 1mM EDTA pH8.0 is recommended for this purpose. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Human | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Membrane permeabilization is recommended for this application. ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species Mouse | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
7 products for Alternative Product
1 product for Alternative Version
Target data
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed:38614099).
Alternative names
CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain
Recommended products
Anti-CD3 antibody CD3-12 is a rat monoclonal antibody that is used in CD3 Western blot, IHC, Flow cytometry. Suitable for Human, Mouse, Rat samples.
- Recombinant format ensures batch-to-batch consistency
- Trusted by researchers since 2004
- Cited in over 100 publications
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human CD3E. The exact immunogen used to generate this antibody is proprietary information. Database link P07766
- Clone number
- CD3-12
- Purification technique
- Affinity purification Protein A
- Specificity
IHC-P is not recommended in Human samples.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Product Specifications
Anti-CD3 antibody [CD3-12] (ab11089) is a rat recombinant monoclonal antibody and is validated for use in in human, mouse, rat samples.
Anti-CD3 antibody [CD3-12] (ab11089) specifically detects CD3 (UniProt ID: P07766; Molecular weight: 21kDa) and is sold in 100 µg and 1 mg selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-CD3 antibody [CD3-12] (ab11089) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD3 antibody [CD3-12] (ab11089) has been cited over 104 times in peer reviewed journals and is trusted by the scientific community.
Anti-CD3 antibody [CD3-12] (ab11089) has 18 independent reviews from customers.
Related Products
Conjugation-ready, carrier free format available for antibody clone CD3-12 - Anti-CD3 antibody [CD3-12] - BSA and Azide free ab255972.
Antibody clone CD3-12 is also available pre-conjugated to a variety of labels for your convenience - FITC (FITC Anti-CD3 antibody [CD3-12] ab34722).
Target Information
CD3, or Cluster of Differentiation 3, is a protein complex and T cell co-receptor that plays a crucial role in the activation of T cells, which are essential for the immune response. In oncology, CD3 is particularly significant due to its involvement in cancer immunotherapy. CD3 is targeted to enhance the body's ability to fight cancer. Bispecific antibodies (bsAbs) that target CD3 and a tumor antigen bring T cells into close proximity with cancer cells, promoting their destruction.
This product has switched from a hybridoma to recombinant production method on 1st November 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089), expandable thumbnail](https://content.abcam.com/products/images/cd3-antibody-cd3-12-ab11089--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441544.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (ab11089)
Immunohistochemical analysis of paraffin-embedded Mouse spleen and kidney tissue labeling CD3 with ab11089 at 5 µg/mL, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.
![Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (ab11089), expandable thumbnail](https://content.abcam.com/products/images/cd3-antibody-cd3-12-ab11089--flow-cytometry-intracellular-img441542.jpg "Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (ab11089)")
Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (ab11089)
Flow cytometric analysis of human peripheral blood mononuclear cells (PMBCs) labelling CD3 with ab11089 at 5 µg/mL (right) compared with a Rat IgG1k isotype control (left).
![Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (ab11089), expandable thumbnail](https://content.abcam.com/products/images/cd3-antibody-cd3-12-ab11089--flow-cytometry-intracellular-img441543.jpg "Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (ab11089)")
Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (ab11089)
Flow cytometric analysis of Mouse spleen cells labelling CD3 with ab11089 at 1 µg/mL (right) compared with a Rat IgG1k isotype control (left).
![Western blot - Anti-CD3 antibody [CD3-12] (ab11089), expandable thumbnail](https://content.abcam.com/products/images/cd3-antibody-cd3-12-ab11089--western-blot-img441545.jpg "Western blot - Anti-CD3 antibody [CD3-12] (ab11089)")
Western blot - Anti-CD3 antibody [CD3-12] (ab11089)
Western blot analysis of ab11089 at 1 µg/mL.
All lanes: Western blot - Anti-CD3 antibody [CD3-12] (ab11089) at 1 µg/mL
Lane 1: Jurkat whole cell lysate at 20 µg
Lane 2: Human thymus tissue lysate at 20 µg
Lane 3: Mouse thymus tissue lysate at 20 µg
Lane 4: Raji whole cell lysate at 20 µg
Lane 5: Human brain tissue lysate at 20 µg
Lane 6: Mouse brain tissue lysate at 20 µg
SecondaryLanes 1 - 6: Goat Anti-Rat IgG H&L (IRDye® 800CW) at 1/20000 dilution
Lanes 1 - 6: Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed at 1/20000 dilution
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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