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AB11089

Anti-CD3 antibody [CD3-12]

5

(18 Reviews)

|

(159 Publications)

Anti-CD3 antibody [CD3-12] (ab11089) is a rat monoclonal antibody detecting CD3 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P. Suitable for Human, Mouse, Rat.

- Over 100 publications
- Trusted since 2004

View Alternative Names

CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (AB11089)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [CD3-12] (AB11089)

Immunohistochemical analysis of paraffin-embedded Mouse spleen and kidney tissue labeling CD3 with ab11089 at 5 µg/mL, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (AB11089)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (AB11089)

Flow cytometric analysis of human peripheral blood mononuclear cells (PMBCs) labelling CD3 with ab11089 at 5 µg/mL (right) compared with a Rat IgG1k isotype control (left).

Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (AB11089)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-CD3 antibody [CD3-12] (AB11089)

Flow cytometric analysis of Mouse spleen cells labelling CD3 with ab11089 at 1 µg/mL (right) compared with a Rat IgG1k isotype control (left).

Western blot - Anti-CD3 antibody [CD3-12] (AB11089)
  • WB

Lab

Western blot - Anti-CD3 antibody [CD3-12] (AB11089)

Western blot analysis of ab11089 at 1 µg/mL.

All lanes:

Western blot - Anti-CD3 antibody [CD3-12] (ab11089) at 1 µg/mL

Lane 1:

Jurkat whole cell lysate at 20 µg

Lane 2:

Human thymus tissue lysate at 20 µg

Lane 3:

Mouse thymus tissue lysate at 20 µg

Lane 4:

Raji whole cell lysate at 20 µg

Lane 5:

Human brain tissue lysate at 20 µg

Lane 6:

Mouse brain tissue lysate at 20 µg

Secondary

Lanes 1 - 6:

Goat Anti-Rat IgG H&L (IRDye® 800CW) at 1/20000 dilution

Lanes 1 - 6:

Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed at 1/20000 dilution

false

  • 519 FITC

    FITC Anti-CD3 antibody [CD3-12]

  • Carrier free

    Anti-CD3 antibody [CD3-12] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

CD3-12

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human CD3E. The exact immunogen used to generate this antibody is proprietary information.

P07766

Specificity

IHC-P is not recommended in Human samples.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p>Membrane permeabilization is recommended for this application. <a href='/en-us/products/unavailable/rat-igg1-kappa-rtk2071-isotype-control-low-endotoxin-azide-free-ab18407'>ab18407</a> - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p>1mM EDTA pH8.0 is recommended for this purpose.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5 µg/mL", "FlowCyt-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

Product Specifications

Anti-CD3 antibody [CD3-12] (ab11089) is a rat recombinant monoclonal antibody and is validated for use in in human, mouse, rat samples.
Anti-CD3 antibody [CD3-12] (ab11089) specifically detects CD3 (UniProt ID: P07766; Molecular weight: 21kDa) and is sold in 100 µg and 1 mg selling sizes.

Quality and Validation

Abcam's high quality manufacturing and validation processes ensure Anti-CD3 antibody [CD3-12] (ab11089) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-CD3 antibody [CD3-12] (ab11089) has been cited over 104 times in peer reviewed journals and is trusted by the scientific community.
Anti-CD3 antibody [CD3-12] (ab11089) has 18 independent reviews from customers.

Related Products

Conjugation-ready, carrier free format available for antibody clone CD3-12 - ab255972.
Antibody clone CD3-12 is also available pre-conjugated to a variety of labels for your convenience - FITC (ab34722).

Target Information

CD3, or Cluster of Differentiation 3, is a protein complex and T cell co-receptor that plays a crucial role in the activation of T cells, which are essential for the immune response. In oncology, CD3 is particularly significant due to its involvement in cancer immunotherapy. CD3 is targeted to enhance the body's ability to fight cancer. Bispecific antibodies (bsAbs) that target CD3 and a tumor antigen bring T cells into close proximity with cancer cells, promoting their destruction.

This product has switched from a hybridoma to recombinant production method on 1st November 2024.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed : 10384095, PubMed : 26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed : 38614099).
See full target information CD3E
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Publications (159)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 9:308 PubMed40897830

2025

Enhanced metastasis risk prediction in cutaneous squamous cell carcinoma using deep learning and computational histopathology.

Applications

Unspecified application

Species

Unspecified reactive species

Emilia Peleva,Yue Chen,Bernhard Finke,Hasan Rizvi,Eugene Healy,Chester Lai,Paul Craig,William Rickaby,Christina Schoenherr,Craig Nourse,Charlotte Proby,Gareth J Inman,Irene M Leigh,Catherine A Harwood,Jun Wang

Frontiers in immunology 16:1578295 PubMed40799649

2025

Renal prognostic value of serum monoclonal immunoglobulin in cryoglobulinemic glomerulonephritis.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Ma,Yuanyuan Xia,Yun Fan,Dan Zhou,Xinchen Yao,Yongzhong Zhong,Fan Yang,Feng Xu,Shaoshan Liang,Yujie Wang,Xiaodong Zhu,Dacheng Chen,Rong Tan,Zhengyun Zhu,Dandan Liang,Caihong Zeng

Nature 644:516-526 PubMed40681898

2025

Pathology-oriented multiplexing enables integrative disease mapping.

Applications

Unspecified application

Species

Unspecified reactive species

Malte Kuehl,Yusuke Okabayashi,Milagros N Wong,Lukas Gernhold,Gabriele Gut,Nico Kaiser,Maria Schwerk,Stefanie K Gräfe,Frank Y Ma,Jovan Tanevski,Philipp S L Schäfer,Sam Mezher,Jacobo Sarabia Del Castillo,Thiago Goldbeck-Strieder,Olga Zolotareva,Michael Hartung,Fernando M Delgado Chaves,Lukas Klinkert,Ann-Christin Gnirck,Marc Spehr,David Fleck,Mehdi Joodaki,Victor Parra,Mina Shaigan,Martin Diebold,Marco Prinz,Jennifer Kranz,Johan M Kux,Fabian Braun,Oliver Kretz,Hui Wu,Florian Grahammer,Sven Heins,Marina Zimmermann,Fabian Haas,Dominik Kylies,Nicola Wanner,Jan Czogalla,Bernhard Dumoulin,Nikolay Zolotarev,Maja Lindenmeyer,Pall Karlson,Jens R Nyengaard,Marcial Sebode,Sören Weidemann,Thorsten Wiech,Hermann-Josef Groene,Nicola M Tomas,Catherine Meyer-Schwesinger,Christoph Kuppe,Rafael Kramann,Alexandre Karras,Patrick Bruneval,Pierre-Louis Tharaux,Diego Pastene,Benito Yard,Jennifer A Schaub,Phillip J McCown,Laura Pyle,Ye Ji Choi,Takashi Yokoo,Jan Baumbach,Pablo J Sáez,Ivan Costa,Jan-Eric Turner,Jeffrey B Hodgin,Julio Saez-Rodriguez,Tobias B Huber,Petter Bjornstad,Matthias Kretzler,Olivia Lenoir,David J Nikolic-Paterson,Lucas Pelkmans,Stefan Bonn,Victor G Puelles

Frontiers in immunology 16:1506204 PubMed40666525

2025

Relaxin-2-secreting CAR-T cells exhibit enhanced efficacy in stromal-rich xenograft tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Hisataka Ogawa,Tomoya Ekawa,Yu Mizote,Takashi Akazawa,Hideaki Tahara

Chemical science 16:14323-14341 PubMed40656537

2025

Degradable water-soluble polymer prodrugs for subcutaneous delivery of irritant anticancer drugs.

Applications

Unspecified application

Species

Unspecified reactive species

Léa Guerassimoff,Jingming Cao,Michaella Auguste,Amaury Bossion,Chen Zhu,Dao Le,Catherine Cailleau,Safa Mohamed Ismail,Françoise Mercier-Nomé,Julien Nicolas

Molecular medicine (Cambridge, Mass.) 31:244 PubMed40542350

2025

Effect of anti-CD4 mAb induced by inhibiting B cell disorder on immune reconstruction of HIV-infected immunological non-responders.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Ouyang,Kang Wu,Lei Fu,Panpan Yi,Da Cheng,Xiaoyu Fu

Cancer immunology, immunotherapy : CII 74:246 PubMed40542851

2025

Immunological synapse formation as a key mechanism in T cell-dependent bispecific antibody-mediated immune activation and cytotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Rikuto Nakamura,Ryo Tsumura,Takahiro Anzai,Ryutaro Asano,Masahiro Yasunaga

Journal of translational medicine 23:653 PubMed40514690

2025

Bidirectional roles of nanoenzymes in enhancing GPC3-CAR T cell infiltration and cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Xu,Jianping Liao,Jiahong Wang,Yuan Gao,Yuan Wu,Meiqin Gao,Wenwen Liu,Da Zhang,Wenmin Zhang,Aimin Huang

The Journal of clinical investigation 135: PubMed40371640

2025

ST6GalNAc-I regulates tumor cell sialylation via NECTIN2/MUC5AC-mediated immunosuppression and angiogenesis in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Muthamil Iniyan Appadurai,Sanjib Chaudhary,Ashu Shah,Gopalakrishnan Natarajan,Zahraa W Alsafwani,Parvez Khan,Dhananjay D Shinde,Subodh M Lele,Lynette M Smith,Mohd Wasim Nasser,Surinder Kumar Batra,Apar Kishor Ganti,Imayavaramban Lakshmanan

Cellular & molecular immunology 22:645-660 PubMed40335738

2025

Harnessing nutrient scarcity for enhanced CAR-T-cell potency and safety in solid tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Enzo Manchon,Nell Hirt,Benjamin Versier,Aravindhan Soundiramourty,Ludmila Juricek,Celeste Lebbe,Maxime Battistella,Yves Christen,Jacques Mallet,Dominique Charron,Nabila Jabrane-Ferrat,Che Serguera,Reem Al-Daccak
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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