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AB255972

Anti-CD3 antibody [CD3-12] - BSA and Azide free

5

(3 Reviews)

|

(4 Publications)

Rat Recombinant Monoclonal CD3E antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain

  • Unconjugated

    Anti-CD3 antibody [CD3-12]

  • 519 FITC

    FITC Anti-CD3 antibody [CD3-12]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

CD3-12

Isotype

IgG1

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/400", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab255972 is the carrier-free version of ab11089.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed : 10384095, PubMed : 26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed : 38614099).
See full target information CD3E
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1040 PubMed40640354

2025

Fortilin deficiency induces anti-atherosclerotic phenotypes in macrophages and protects hypercholesterolemic mice against atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Nattaporn Wanachottrakul,Decha Pinkaew,Sandipan Mukherjee,Preedakorn Chunhacha,Mari Nakashima,Asa A Brockman,Uttariya Pal,Hasseri B Halim,Yuhong Wei,Lena Tanaka,Hanna Huynh,Kota Ramana,Shiyou Chen,Rebecca A Ihrie,Ken Fujise

Frontiers in immunology 16:1569490 PubMed40297579

2025

Multiplex immunofluorescence assessment of macrophages and IL-23R in inflammatory and malignant diseases of the oral mucosa: a pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Leah Trumet,Bettina Grötsch,Abbas Agaimy,Kerstin Galler,Carol Geppert,Linus Winter,Jutta Ries,Marco Kesting,Manuel Weber

Cell reports. Medicine 5:101469 PubMed38508137

2024

DNAJB1-PRKACA fusion neoantigens elicit rare endogenous T cell responses that potentiate cell therapy for fibrolamellar carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Allison M Kirk,Jeremy Chase Crawford,Ching-Heng Chou,Cliff Guy,Kirti Pandey,Tanya Kozlik,Ravi K Shah,Shanzou Chung,Phuong Nguyen,Xiaoyu Zhang,Jin Wang,Matthew Bell,Robert C Mettelman,E Kaitlynn Allen,Mikhail V Pogorelyy,Hyunjin Kim,Anastasia A Minervina,Walid Awad,Resha Bajracharya,Toni White,Donald Long,Brittney Gordon,Michelle Morrison,Evan S Glazer,Andrew J Murphy,Yixing Jiang,Elizabeth A Fitzpatrick,Mark Yarchoan,Praveen Sethupathy,Nathan P Croft,Anthony W Purcell,Sara M Federico,Elizabeth Stewart,Stephen Gottschalk,Anthony E Zamora,Christopher DeRenzo,Scott E Strome,Paul G Thomas

Cell & bioscience 14:19 PubMed38311785

2024

Tunable PhenoCycler imaging of the murine pre-clinical tumour microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Madelyn J Abraham,Christophe Goncalves,Paige McCallum,Vrinda Gupta,Samuel E J Preston,Fan Huang,Hsiang Chou,Natascha Gagnon,Nathalie A Johnson,Wilson H Miller,Koren K Mann,Sonia V Del Rincon
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com