AB17143

Anti-CD3 antibody [F7.2.38]

Name: Anti-CD3 antibody [F7.2.38] (ab17143) | Abcam
Brand: Abcam Limited
SKU: AB17143
Rating: 4 (6 reviews)

Lab Essentials
What is this?

(6 Reviews)

(39 Publications)

Anti-CD3 antibody [F7.2.38] (ab17143) is a mouse monoclonal antibody detecting CD3 in IHC-P. Suitable for Human.

- Over 20 publications
- Trusted since 2005

View Alternative Names

CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [F7.2.38] (AB17143)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [F7.2.38] (AB17143)

Paraffin embedded human tonsil tissue stained for CD3 using ab17143 in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [F7.2.38] (AB17143)

ab17143 staining CD3 in formalin-fixed, paraffin-embedded Human tonsil tissue by Immunohistochemistry.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

F7.2.38

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Native Full Length Protein corresponding to Human CD3E.

P07766

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10 - 1/25", "IHCP-species-notes": "<p>We suggest an incubation period of 30-60 minutes at room temperature.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }

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Product details

What is this antibody validated in?
Anti-CD3 antibody [F7.2.38] (ab17143) is a mouse monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Trusted by the scientific community
Anti-CD3 [F7.2.38] (ab17143) was first used in a scientific publication in 2005 and has been cited over 20 times in peer-reviewed journals.

Reviewed by scientists
Anti-CD3 [F7.2.38] (ab17143) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: Tissue culture supernatant, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed : 10384095, PubMed : 26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed : 38614099).

See full target information CD3E

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Publications (39)

Recent publications for all applications. Explore the full list and refine your search

European stroke journal 10:892-901 PubMed39910895

2025

C-reactive protein expression in acute ischemic stroke blood clots: Implications for etiology.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyi Liu,Cansu Sahin,Nazan Güner Sak,Alice Giraud,Pierluca Messina,Franz Bozsak,Jean Darcourt,Federico Sacchetti,Anne-Christine Januel,Guillaume Bellanger,Jorge Pagola,Jesus Juega,Hirotoshi Imamura,Tsuyoshi Ohta,Laurent Spelle,Vanessa Chalumeau,Uros Mircic,Predrag Stanarčević,Ivan Vukašinović,Marc Ribo,Nobuyuki Sakai,Christophe Cognard,Karen Doyle

Haematologica 110:1339-1350 PubMed39665214

2024

Characterization of tumor microenvironment and cell interaction patterns in testicular and diffuse large B-cell lymphomas.

Applications

Unspecified application

Species

Unspecified reactive species

Matias Autio,Oscar Brück,Marjukka Pollari,Marja-Liisa Karjalainen-Lindsberg,Klaus Beiske,Judit Mészaros Jørgensen,Harald Holte,Teijo Pellinen,Suvi-Katri Leivonen,Sirpa Leppä

Vision research 226:108519 PubMed39549467

2024

Intravitreal AAV2 gene delivery to feline retinal ganglion cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuya Oikawa,J Seth Eaton,Julie A Kiland,Odalys Torné,Virginia Mathu,Robert W Nickells,Gillian J McLellan

Experimental dermatology 33:e15062 PubMed38532566

2024

Analyses of tertiary lymphoid structures observed in cases of Merkel cell carcinoma showing spontaneous regression.

Applications

Unspecified application

Species

Unspecified reactive species

Motoki Nakamura,Maki Yoshimitsu,Tetsuya Magara,Shinji Kano,Hiroshi Kato,Akimichi Morita

Arthritis & rheumatology (Hoboken, N.J.) 76:1230-1242 PubMed38508862

2024

Synovial 5-Lipoxygenase-Derived Oxylipins Define a Lympho-Myeloid-Enriched Synovium.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica D Murillo-Saich,Roxana Coras,Julio Ramirez,Estefania Quesada-Masachs,Marta Sala-Climent,Katharina Eschelbach,Christopher B Mahony,Raquel Celis,Aaron Armando,Oswald Quehenberger,Adam P Croft,Arthur Kavanaugh,Eric Chang,Juan D Cañete,Abha Singh,Monica Guma

International journal of molecular sciences 25: PubMed38474245

2024

Investigating the Role of Brain Natriuretic Peptide (BNP) and N-Terminal-proBNP in Thrombosis and Acute Ischemic Stroke Etiology.

Applications

Unspecified application

Species

Unspecified reactive species

Rosanna Rossi,Duaa Jabrah,Andrew Douglas,James Prendergast,Abhay Pandit,Michael Gilvarry,Ray McCarthy,Petra Redfors,Annika Nordanstig,Turgut Tatlisumak,Erik Ceder,Dennis Dunker,Jeanette Carlqvist,István Szikora,Georgios Tsivgoulis,Klearchos Psychogios,John Thornton,Alexandros Rentzos,Katarina Jood,Jesus Juega,Karen M Doyle

International journal of molecular sciences 25: PubMed38203757

2024

Comparative Evaluation of STEAP1 Targeting Chimeric Antigen Receptors with Different Costimulatory Domains and Spacers.

Applications

Unspecified application

Species

Unspecified reactive species

Yixin Jin,Claire Dunn,Irene Persiconi,Adam Sike,Gjertrud Skorstad,Carole Beck,Jon Amund Kyte

Journal of nuclear medicine : official publication, Society of Nuclear Medicine 64:1056-1061 PubMed37024303

2023

Addition of Peptide Receptor Radiotherapy to Immune Checkpoint Inhibition Therapy Improves Outcomes in Neuroendocrine Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Shadi A Esfahani,Carolina De Aguiar Ferreira,Priska Summer,Umar Mahmood,Pedram Heidari

Frontiers in oncology 13:1106434 PubMed37081973

2023

Tumor immune microenvironment of cutaneous angiosarcoma with cancer testis antigens and the formation of tertiary lymphoid structures.

Applications

Unspecified application

Species

Unspecified reactive species

Tetsuya Magara,Motoki Nakamura,Yuka Nojiri,Maki Yoshimitsu,Shinji Kano,Hiroshi Kato,Akimichi Morita

Acta dermato-venereologica 103:adv4463 PubMed36967545

2023

Neuroimmune Mediators of Pruritus in Hispanic Scalp Psoriatic Itch.

Applications

Unspecified application

Species

Unspecified reactive species

Leigh A Nattkemper,Zoe M Lipman,Giuseppe Ingrasci,Claudia Maldonado,Juan Carlos Garces,Enrique Loayza,Gil Yosipovitch

View all publications

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Product promise

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