AB25109

Anti-CD3 antibody [FN-18]

Name: Anti-CD3 antibody [FN-18] (ab25109) | Abcam
Brand: Abcam Limited
SKU: AB25109
Price: 400 USD
Availability: InStock
Rating: 1 (1 reviews)

Lab Essentials
What is this?

(1 Review)

(2 Publications)

Mouse Monoclonal CD3d antibody. Suitable for IHC-Fr and reacts with Rhesus monkey samples. Cited in 2 publications. Immunogen corresponding to Cell preparation containing CD3D protein.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

1 Images

Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [FN-18] (AB25109)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [FN-18] (AB25109)

Immunohistochemistry (Frozen sections) analysis of lymph node tissue from Rhesus macaque labeling CD3 with ab25109 at 1/100 dilution. Tissue was fixed in acetone, blocked using 10% goat serum/PBS for 15 min at 21°C, then incubated with Anti-CD3 antibody [FN-18] (ab25109) at 1/100 dilution for 16 hours at 4°C. An undiluted HRP-conjugated goat anti-mouse polyclonal was used as the secondary antibody.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

FN-18

Isotype

IgG1

Carrier free

Reacts with

Rhesus monkey

Applications

IHC-Fr

applications

Immunogen

Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information.

P04234

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "" }, "Mouse": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "" }, "Rat": { "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "" }, "Rhesus monkey": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification notes

Ion exchange chromatography

Storage buffer

Constituents: PBS, 4.275% Trehalose

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).

See full target information CD3D

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of leukocyte biology 96:957-67 PubMed25114162

2014

HMBPP-deficient Listeria mutant immunization alters pulmonary/systemic responses, effector functions, and memory polarization of Vγ2Vδ2 T cells.

Applications

Unspecified application

Species

Unspecified reactive species

James T Frencher,Hongbo Shen,Lin Yan,Jessica O Wilson,Nancy E Freitag,Alicia N Rizzo,Crystal Y Chen,Zheng W Chen

Journal of immunology (Baltimore, Md. : 1950) 179:3325-31 PubMed17709549

2007

"Cytokine storm" in the phase I trial of monoclonal antibody TGN1412: better understanding the causes to improve preclinical testing of immunotherapeutics.

Applications

Flow Cyt

Species

Cynomolgus monkey

Richard Stebbings,Lucy Findlay,Cherry Edwards,David Eastwood,Chris Bird,David North,Yogesh Mistry,Paula Dilger,Emily Liefooghe,Isabelle Cludts,Bernard Fox,Gill Tarrant,Jane Robinson,Tony Meager,Carl Dolman,Susan J Thorpe,Adrian Bristow,Meenu Wadhwa,Robin Thorpe,Stephen Poole

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Product promise

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