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AB33429

Anti-CD3 antibody [KT3]

4

(3 Reviews)

|

(10 Publications)

Rat Monoclonal CD3d antibody. Suitable for Flow Cyt, ICC/IF, IHC-Fr and reacts with Mouse samples. Cited in 10 publications.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

1 Images
Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [KT3] (AB33429)
  • IHC-Fr

AbReview18522****

Immunohistochemistry (Frozen sections) - Anti-CD3 antibody [KT3] (AB33429)

ab33429 staining CD3 in mouse spleen tissue by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/250 dilution for 12 hours at 4°C. A goat anti-rat IgG conjugated to Alexa Fluor®488 was used as secondary antibody at a 1/250 dilution.

Image courtesy of an anonymous Abreview

  • 578 PE

    PE Anti-CD3 antibody [KT3]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

KT3

Isotype

IgG2a

Carrier free

No

Reacts with

Mouse

Applications

ICC/IF, IHC-Fr, Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450'>ab18450</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

ab33429 may be used to trigger proliferation and cytotoxicity of CD3 positive cells.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 33:102045 PubMed40688666

2025

Dendritic cell membrane-based DCsLipo@MnO@siCTLA4@PD-1α nanomedicine for the treatment of Lynch syndrome-related colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjin Zhong,Pengcheng Wang,Jintian Wang,Yuejia Zhu,Liquan Chen,Yilong Qian,Xiang Huang,Kai Ye

Frontiers in immunology 15:1466802 PubMed39840066

2025

Restoring natural killer cell activity in lung injury with 1,25-hydroxy vitamin D: a promising therapeutic approach.

Applications

Unspecified application

Species

Unspecified reactive species

Johnny Amer,Ahmad Salhab,Mohammad Abuawad

Experimental and therapeutic medicine 26:568 PubMed37954119

2023

CD138 promotes the accumulation and activation of autoreactive T cells in autoimmune MRL/lpr mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tianhong Xie,Xin Liu,Ping Li

Bioengineering & translational medicine 7:e10316 PubMed36176616

2022

Ex vivo anchored PD-L1 functionally prevent in vivo renal allograft rejection.

Applications

Unspecified application

Species

Unspecified reactive species

Zihuan Luo,Tao Liao,Yannan Zhang,Haofeng Zheng,Qipeng Sun,Fei Han,Maolin Ma,Yongrong Ye,Qiquan Sun

Nature 590:473-479 PubMed33408417

2021

Gut-licensed IFNγ NK cells drive LAMP1TRAIL anti-inflammatory astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Liliana M Sanmarco,Michael A Wheeler,Cristina Gutiérrez-Vázquez,Carolina Manganeli Polonio,Mathias Linnerbauer,Felipe A Pinho-Ribeiro,Zhaorong Li,Federico Giovannoni,Katelyn V Batterman,Giulia Scalisi,Stephanie E J Zandee,Evelyn S Heck,Moneera Alsuwailm,Douglas L Rosene,Burkhard Becher,Isaac M Chiu,Alexandre Prat,Francisco J Quintana

CNS neuroscience & therapeutics 26:1288-1302 PubMed32790044

2020

Poldip2 mediates blood-brain barrier disruption and cerebral edema by inducing AQP4 polarity loss in mouse bacterial meningitis model.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Gao,Weitian Lu,Yue Shu,Zhengyu Yang,Shanquan Sun,Jin Xu,Shengwei Gan,Shujuan Zhu,Guoping Qiu,Fei Zhuo,Shiye Xu,Yiying Wang,Junhong Chen,Xuan Wu,Juan Huang

American journal of physiology. Gastrointestinal a 317:G625-G639 PubMed31545917

2019

Proteomics analysis of the matrisome from MC38 experimental mouse liver metastases.

Applications

Unspecified application

Species

Unspecified reactive species

Arseniy E Yuzhalin,Su Yin Lim,Alex N Gordon-Weeks,Roman Fischer,Benedikt M Kessler,Dihua Yu,Ruth J Muschel

Clinical cancer research : an official journal of 25:3289-3303 PubMed30755440

2019

Immunotherapeutic Blockade of Macrophage Clever-1 Reactivates the CD8 T-cell Response against Immunosuppressive Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Miro Viitala,Reetta Virtakoivu,Sina Tadayon,Jenna Rannikko,Sirpa Jalkanen,Maija Hollmén

ALTEX 36:29-38 PubMed30076761

2018

An ex vivo porcine spleen perfusion as a model of bacterial sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Y Chung,Joseph J Wanford,Rohan Kumar,John D Isherwood,Richard D Haigh,Marco R Oggioni,Ashley R Dennison,Giuseppe Ercoli

The Journal of experimental medicine 211:929-42 PubMed24752297

2014

Leucine-rich repeat containing 8A (LRRC8A) is essential for T lymphocyte development and function.

Applications

Unspecified application

Species

Unspecified reactive species

Lalit Kumar,Janet Chou,Christina S K Yee,Arturo Borzutzky,Elisabeth H Vollmann,Ulrich H von Andrian,Shin-Young Park,Georg Hollander,John P Manis,P Luigi Poliani,Raif S Geha
View all publications
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Product promise

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