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AB8090

Anti-CD3 antibody [MEM-57]

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(10 Publications)

Mouse Monoclonal CD3d antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Cell preparation containing CD3D protein.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

4 Images
Flow Cytometry - Anti-CD3 antibody [MEM-57] (AB8090)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD3 antibody [MEM-57] (AB8090)

Flow cytometry analysis of human peripheral whole blood labeling CD3 with ab8090 at 0.33 μg/mL (GAM APC).

Flow Cytometry - Anti-CD3 antibody [MEM-57] (AB8090)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD3 antibody [MEM-57] (AB8090)

Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD3 (MEM-57) Dy161. Gated on singlets.

Flow Cytometry - Anti-CD3 antibody [MEM-57] (AB8090)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD3 antibody [MEM-57] (AB8090)

Flow cytometry analysis of human peripheral whole blood labeling CD3 with ab8090 at 0.33 μg/mL (GAM APC). Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed).

Flow Cytometry - Anti-CD3 antibody [MEM-57] (AB8090)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-CD3 antibody [MEM-57] (AB8090)

Human peripheral blood lymphocytes stained with ab8090 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID : 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MEM-57

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information.

P04234

Specificity

This antibody reacts with epsilon-gamma and epsilon-delta dimers of human CD3 complex, a part of a bigger multisubunit complex of the T cell receptor for antigen (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes. It reacts more strongly with blood gamma-delta T lymphocytes than with alpha-beta T lymphocytes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "2-5 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Activates T cells in vitro. This antibody is a highly effective immunosuppressive agent. In this it is similar to OKT3.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
This product has been purified from tissue culture supernatant.
Storage buffer
pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 13:2922-2937 PubMed37559979

2023

Indisulam exerts anticancer effects via modulation of transcription, translation and alternative splicing on human cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhihui Dou,Xuetian Zhang,Wei Su,Taotao Zhang,Fei Ye,Dapeng Zhao,Xiaohua Chen,Qiang Li,Hong Zhang,Cuixia Di

Cell cycle (Georgetown, Tex.) 21:2533-2549 PubMed35894701

2022

Prognostic values of the immune microenvironment-related non-coding RNA IGF2BP2-AS1 in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Ding,Zhihuan Zheng,Yu Han,Xiangyun Huang

Methods and protocols 2: PubMed31164624

2019

Induction of a Th17 Phenotype in Human Skin-A Mimic of Dermal Inflammatory Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Sara M Garrett,Qihong Zhao,Carol Feghali-Bostwick

Cell 173:260-274.e25 PubMed29551266

2018

Multiplexed Proteome Dynamics Profiling Reveals Mechanisms Controlling Protein Homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Mikhail M Savitski,Nico Zinn,Maria Faelth-Savitski,Daniel Poeckel,Stephan Gade,Isabelle Becher,Marcel Muelbaier,Anne J Wagner,Katrin Strohmer,Thilo Werner,Stephanie Melchert,Massimo Petretich,Anna Rutkowska,Johanna Vappiani,Holger Franken,Michael Steidel,Gavain M Sweetman,Omer Gilan,Enid Y N Lam,Mark A Dawson,Rab K Prinjha,Paola Grandi,Giovanna Bergamini,Marcus Bantscheff

PloS one 11:e0153578 PubMed27077658

2016

Impact of the New Generation Reconstituted Surfactant CHF5633 on Human CD4+ Lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Markus Fehrholz,Kirsten Glaser,Silvia Seidenspinner,Barbara Ottensmeier,Tore Curstedt,Christian P Speer,Steffen Kunzmann

PLoS pathogens 10:e1004189 PubMed24991932

2014

Tetherin can restrict cell-free and cell-cell transmission of HIV from primary macrophages to T cells.

Applications

Flow Cyt

Species

Unspecified reactive species

Sebastian Giese,Mark Marsh

PloS one 9:e85934 PubMed24454946

2014

Lck mediates signal transmission from CD59 to the TCR/CD3 pathway in Jurkat T cells.

Applications

Unspecified application

Species

Human

Anna M Lipp,Kata Juhasz,Christian Paar,Christoph Ogris,Paul Eckerstorfer,Roland Thuenauer,Jan Hesse,Benedikt Nimmervoll,Hannes Stockinger,Gerhard J Schütz,Ulrich Bodenhofer,Zsolt Balogi,Alois Sonnleitner

Nature chemical biology 8:576-82 PubMed22544264

2012

A selective inhibitor reveals PI3Kγ dependence of T(H)17 cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanna Bergamini,Kathryn Bell,Satoko Shimamura,Thilo Werner,Andrew Cansfield,Katrin Müller,Jessica Perrin,Christina Rau,Katie Ellard,Carsten Hopf,Carola Doce,Daniel Leggate,Raffaella Mangano,Toby Mathieson,Alison O'Mahony,Ivan Plavec,Faiza Rharbaoui,Friedrich Reinhard,Mikhail M Savitski,Nigel Ramsden,Emilio Hirsch,Gerard Drewes,Oliver Rausch,Marcus Bantscheff,Gitte Neubauer

Transplantation 55:435-8 PubMed8434399

1993

Therapeutic in vivo use of the A1-CD3 monoclonal antibody.

Applications

Unspecified application

Species

Unspecified reactive species

I Hilgert,F Franĕk,I Stefanová,J Kaslík,J Jirka,H Kristofová,P Rossmann,J Soucek,V Horejsi

Folia biologica 34:23-34 PubMed2968928

1988

Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).

Applications

Unspecified application

Species

Unspecified reactive species

V Horejsí,P Angelisová,V Bazil,H Kristofová,S Stoyanov,I Stefanová,P Hausner,M Vosecký,I Hilgert
View all publications
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