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Mouse Monoclonal CD3d antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing CD3D protein.

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Images

Flow Cytometry - Anti-CD3 antibody [MEM-57] (AB8090), expandable thumbnail
  • Flow Cytometry - Anti-CD3 antibody [MEM-57] (AB8090), expandable thumbnail
  • Flow Cytometry - Anti-CD3 antibody [MEM-57] (AB8090), expandable thumbnail
  • Flow Cytometry - Anti-CD3 antibody [MEM-57] (AB8090), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information. Database link P04234

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt
Human
Tested

Tested
Tested

Species
Human
Dilution info
2.00000-5.00000 µg/mL
Notes

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Associated Products

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Target data

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed:12215456).

Alternative names

Recommended products

Mouse Monoclonal CD3d antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Cell preparation containing CD3D protein.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information. Database link P04234
Clone number
MEM-57
Purification technique
Affinity purification Protein A
Specificity

This antibody reacts with epsilon-gamma and epsilon-delta dimers of human CD3 complex, a part of a bigger multisubunit complex of the T cell receptor for antigen (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes. It reacts more strongly with blood gamma-delta T lymphocytes than with alpha-beta T lymphocytes.

Light chain type
kappa
Concentration
Loading...
Purification notes

This product has been purified from tissue culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Activates T cells in vitro. This antibody is a highly effective immunosuppressive agent. In this it is similar to OKT3.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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4 product images

  • Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090), expandable thumbnail

    Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)

    Human peripheral blood lymphocytes stained with ab8090 (red line). Human whole blood was processed using a modified protocol based on Chow et al, 2005 (PMID: 16080188). In brief, human whole blood was fixed in 4% formaldehyde (methanol-free) for 10 min at 22°C. Red blood cells were then lyzed by the addition of Triton X-100 (final concentration - 0.1%) for 15 min at 37°C. For experimentation, cells were treated with 50% methanol (-20°C) for 15 min at 4°C. Cells were then incubated with the antibody (ab8090, 0.01μg/1x106 cells) for 30 min at 4°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) at 1/2000 dilution for 30 min at 4°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >30,000 total events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Gating strategy - peripheral blood lymphocytes.

  • Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090), expandable thumbnail

    Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)

    Flow cytometry analysis of human peripheral whole blood labeling CD3 with ab8090 at 0.33 μg/mL (GAM APC).

  • Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090), expandable thumbnail

    Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)

    Flow cytometry analysis of human peripheral whole blood labeling CD3 with ab8090 at 0.33 μg/mL (GAM APC). Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed).

  • Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090), expandable thumbnail

    Flow Cytometry - Anti-CD3 antibody [MEM-57] (ab8090)

    Surface staining (mass cytometry) of PBMC after Ficoll-Paque separation with anti-human CD3 (MEM-57) Dy161. Gated on singlets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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