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AB86883

Anti-CD3 antibody [OKT3]

5

(1 Review)

|

(14 Publications)

Mouse Recombinant Monoclonal CD3d antibody. Suitable for Flow Cyt and reacts with Human samples. Cited in 14 publications.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD3 antibody [OKT3]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD3 antibody [OKT3]

  • redFluor™ 710

    redFluor™ 710 Anti-CD3 antibody [OKT3]

  • 660 APC

    APC Anti-CD3 epsilon antibody [OKT3]

  • Biotin

    Biotin Anti-CD3 antibody [OKT3]

  • 519 FITC

    FITC Anti-CD3 epsilon antibody [OKT3]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OKT3

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "FuncSAct" : {"fullname" : "Functional Studies (Act)", "shortname":"FuncS (Act)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>", "FuncSAct-species-checked": "notRecommended", "FuncSAct-species-dilution-info": "", "FuncSAct-species-notes": "<p>(Activation)</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 0.87% Sodium chloride, 0.14% Sodium dihydrogen phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1597779 PubMed40458393

2025

The novel role of Yin Yang 1 in acute rejection of liver allografts through activation of dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Chen,Jie Wang,Liping Hong,Hongtao Wang,Wubing He,Lihong Chen

Journal for immunotherapy of cancer 12: PubMed38688579

2024

ScRNA-seq reveals novel immune-suppressive T cells and investigates CMV-TCR-T cells cytotoxicity against GBM.

Applications

Unspecified application

Species

Unspecified reactive species

Xinmiao Long,Zuping Zhang,Yuzhe Li,Kun Deng,Wei Gao,Meng Huang,Xiangyu Wang,Xiang Lin,Xiaoling She,Yiming Zhao,Minfu Zhang,Cheng Huang,Shiyi Wang,Yinfei Du,Peng Du,Shuai Chen,Qing Liu,Minghua Wu

Methods in molecular biology (Clifton, N.J.) 2789:109-120 PubMed38506996

2024

Detection of Intracellular Complement Activation by Nanoparticles in Human T Lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Ilinskaya,Ankit Shah,Andrew Van Dusen,Marina A Dobrovolskaia

Nature communications 14:2071 PubMed37045832

2023

Low-dose IL-2 enhances the generation of IL-10-producing immunoregulatory B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Akimichi Inaba,Zewen Kelvin Tuong,Tian X Zhao,Andrew P Stewart,Rebeccah Mathews,Lucy Truman,Rouchelle Sriranjan,Jane Kennet,Kourosh Saeb-Parsy,Linda Wicker,Frank Waldron-Lynch,Joseph Cheriyan,John A Todd,Ziad Mallat,Menna R Clatworthy

Biomedical reports 16:3 PubMed34820126

2021

Evaluation of skin sensitization based on interleukin-2 promoter activation in Jurkat cells.

Applications

Unspecified application

Species

Unspecified reactive species

Taichi Nagahata,Yoshio Tsujino,Eiji Takayama,Haruka Hikasa,Ayano Satoh

Frontiers in immunology 12:679398 PubMed34177922

2021

High Mobility Group Box 1 Contributes to the Acute Rejection of Liver Allografts by Activating Dendritic Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Chen,Wenmin Zhang,Hui Bao,Wubing He,Lihong Chen

Nature methods 18:84-91 PubMed33398190

2021

Cell surface thermal proteome profiling tracks perturbations and drug targets on the plasma membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Mathias Kalxdorf,Ina Günthner,Isabelle Becher,Nils Kurzawa,Sascha Knecht,Mikhail M Savitski,H Christian Eberl,Marcus Bantscheff

Cellular signalling 58:20-33 PubMed30831195

2019

Regulation of endothelial cell survival and death by the MAP kinase/ERK kinase kinase 3 - glyceraldehyde-3-phosphate dehydrogenase signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Q Li,Andy Ngo,Peter Hoffmann,Antonio Ferrante,Charles S Hii

Nanomedicine : nanotechnology, biology, and medici 17:266-275 PubMed30794962

2019

Nanoparticle physicochemical properties determine the activation of intracellular complement.

Applications

Unspecified application

Species

Unspecified reactive species

Anna N Ilinskaya,Ankit Shah,Alan E Enciso,King C Chan,Jan A Kaczmarczyk,Josip Blonder,Eric E Simanek,Marina A Dobrovolskaia

The journals of gerontology. Series A, Biological sciences and medical sciences 71:1124-30 PubMed26525092

2015

Association Between Circulating Osteogenic Progenitor Cells and Disability and Frailty in Older Persons: The Nepean Osteoporosis and Frailty Study.

Applications

Unspecified application

Species

Unspecified reactive species

Piumali Gunawardene,Sandra Bermeo,Christopher Vidal,Ahmed Al-Saedi,Philip Chung,Derek Boersma,Steven Phu,Izabella Pokorski,Pushpa Suriyaarachchi,Oddom Demontiero,Gustavo Duque
View all publications
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