Mouse Monoclonal CD3d antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human samples. Cited in 39 publications. Immunogen corresponding to Recombinant Protein within Human CD3D.
IgG2a
Mouse
Preservative: 0.09% Sodium azide
Constituents: 97.5% Water, 0.99% BSA, 0.33% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.007% Hydrochloric acid
Liquid
Monoclonal
WB | IHC-P | ICC/IF | IHC-Fr | |
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Human | Expected | Expected | Expected | Expected |
Species | Dilution info | Notes |
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Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Select an associated product type
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed:12215456).
T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain
Mouse Monoclonal CD3d antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human samples. Cited in 39 publications. Immunogen corresponding to Recombinant Protein within Human CD3D.
IgG2a
Mouse
Preservative: 0.09% Sodium azide
Constituents: 97.5% Water, 0.99% BSA, 0.33% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.007% Hydrochloric acid
Liquid
Monoclonal
PS1
Affinity purification
This antibody, when used in conjunction with UCHL-1, is regarded as a reliable pan T-cell marker for immunophenotyping of lymphomas (See reference). Exceptions include some of the more aggressive, high-grade T cell lymphomas, which may not express detectable antigen. This antibody does not react with B-cells.
unknown
Blue Ice
1-2 weeks
+4°C
-20°C
Avoid freeze / thaw cycle
This antibody works well when used in combination with ab972 - Antigen retrieval solution - heat mediated high pH 9.5.
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