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Mouse Monoclonal CD3d antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human samples. Cited in 39 publications. Immunogen corresponding to Recombinant Protein within Human CD3D.


Publications

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 97.5% Water, 0.99% BSA, 0.33% Tris HCl, 0.04% Sorbitan monolaurate, ethoxylated, 0.007% Hydrochloric acid

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Protein within Human CD3D. The exact immunogen used to generate this antibody is proprietary information. Database link P04234

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFIHC-Fr
Human
Expected
Expected
Expected
Expected

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed:12215456).

Alternative names

Recommended products

Mouse Monoclonal CD3d antibody. Suitable for WB, IHC-P, ICC/IF, IHC-Fr and reacts with Human samples. Cited in 39 publications. Immunogen corresponding to Recombinant Protein within Human CD3D.

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Protein within Human CD3D. The exact immunogen used to generate this antibody is proprietary information. Database link P04234
Clone number

PS1

Purification technique

Affinity purification

Specificity

This antibody, when used in conjunction with UCHL-1, is regarded as a reliable pan T-cell marker for immunophenotyping of lymphomas (See reference). Exceptions include some of the more aggressive, high-grade T cell lymphomas, which may not express detectable antigen. This antibody does not react with B-cells.

Light chain type

unknown

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

Notes

This antibody works well when used in combination with ab972 - Antigen retrieval solution - heat mediated high pH 9.5.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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