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AB56313

Anti-CD3 antibody [RM0027-3B19]

4

(2 Reviews)

|

(14 Publications)

Rat Monoclonal CD3d antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 14 publications. Immunogen corresponding to Cell preparation containing Cd3d protein.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [RM0027-3B19] (AB56313)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [RM0027-3B19] (AB56313)

ab56313 at 1/400 dilution staining CD3 in LPS treated mouse spleen sections by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [RM0027-3B19] (AB56313)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [RM0027-3B19] (AB56313)

ab56313, staining Mouse CD3 in Kidney, using Immunohistochemistry, Paraffin embedded tissue.

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

RM0027-3B19

Isotype

IgG2b

Carrier free

No

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

Cell preparation containing Cd3d protein. The exact immunogen used to generate this antibody is proprietary information.

P04235

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/400", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).
See full target information CD3D
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 222: PubMed40266307

2025

Microglia and CD8+ T cell activation precede neuronal loss in a murine model of spastic paraplegia 15.

Applications

Unspecified application

Species

Unspecified reactive species

Aleksej Frolov,Hao Huang,Dagmar Schütz,Maren Köhne,Nelli Blank-Stein,Collins Osei-Sarpong,Maren Büttner,Tarek Elmzzahi,Mukhran Khundadze,Marina Zahid,Michael Reuter,Matthias Becker,Elena De Domenico,Lorenzo Bonaguro,Axel Kallies,Helen Morrison,Christian A Hübner,Kristian Händler,Ralf Stumm,Elvira Mass,Marc D Beyer

Molecular therapy : the journal of the American Society of Gene Therapy 30:1227-1238 PubMed34933101

2021

Tim-3 regulates sepsis-induced immunosuppression by inhibiting the NF-κB signaling pathway in CD4 T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Siyuan Huang,Di Liu,Jianhui Sun,Huacai Zhang,Jing Zhang,Qiang Wang,Lebin Gan,Guoxin Qu,Jinchao Qiu,Jin Deng,Jianxin Jiang,Ling Zeng

JCI insight 6: PubMed34236044

2021

Imaging alloreactive T cells provides early warning of organ transplant rejection.

Applications

Unspecified application

Species

Unspecified reactive species

Toshihito Hirai,Aaron T Mayer,Tomomi W Nobashi,Po-Yu Lin,Zunyu Xiao,Tomokatsu Udagawa,Kinya Seo,Federico Simonetta,Jeanette Baker,Alan G Cheng,Robert S Negrin,Sanjiv S Gambhir

Theranostics 10:10619-10633 PubMed32929370

2020

CDK4/6 inhibition promotes immune infiltration in ovarian cancer and synergizes with PD-1 blockade in a B cell-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Qian-Feng Zhang,Jia Li,Kuo Jiang,Rui Wang,Jun-Li Ge,Hong Yang,Shu-Juan Liu,Lin-Tao Jia,Lei Wang,Bi-Liang Chen

Molecular therapy. Nucleic acids 16:650-662 PubMed31121477

2019

Targeting Lymph Node Sinus Macrophages to Inhibit Lymph Node Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Junqing Hu,Jinhao Xu,Mingyue Li,Yanping Zhang,Huaiqiang Yi,Jiangning Chen,Lei Dong,Junfeng Zhang,Zhen Huang

Frontiers in immunology 9:2091 PubMed30319603

2018

Polyphyllin I Ameliorates Collagen-Induced Arthritis by Suppressing the Inflammation Response in Macrophages Through the NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Wang,Xin Zhou,Yongjian Zhao,Jun Xiao,Yao Lu,Qi Shi,Yongjun Wang,Hongyan Wang,Qianqian Liang

Clinical and experimental immunology 193:113-129 PubMed29570768

2018

Tim-3 exacerbates kidney ischaemia/reperfusion injury through the TLR-4/NF-κB signalling pathway and an NLR-C4 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Y Guo,J Zhang,X Lai,M Chen,Y Guo

Scientific reports 8:2135 PubMed29391506

2018

Therapeutic effects of anti-CD154 antibody in cynomolgus monkeys with advanced rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Wha Choi,Kyo Won Lee,Hyojun Park,Hwajung Kim,Jong Hyun Lee,Ji Woo Song,Jehoon Yang,Yeongbeen Kwon,Tae Min Kim,Jae Berm Park,Sungjoo Kim

Scientific reports 8:2039 PubMed29391517

2018

Curcumin targets multiple enzymes involved in the ROS metabolic pathway to suppress tumor cell growth.

Applications

IHC-P

Species

Unspecified reactive species

Yonika Arum Larasati,Noriko Yoneda-Kato,Ikuko Nakamae,Takashi Yokoyama,Edy Meiyanto,Jun-Ya Kato

Current biology : CB 27:3302-3314.e6 PubMed29112861

2017

Long-Fiber Carbon Nanotubes Replicate Asbestos-Induced Mesothelioma with Disruption of the Tumor Suppressor Gene Cdkn2a (Ink4a/Arf).

Applications

Unspecified application

Species

Unspecified reactive species

Tatyana Chernova,Fiona A Murphy,Sara Galavotti,Xiao-Ming Sun,Ian R Powley,Stefano Grosso,Anja Schinwald,Joaquin Zacarias-Cabeza,Kate M Dudek,David Dinsdale,John Le Quesne,Jonathan Bennett,Apostolos Nakas,Peter Greaves,Craig A Poland,Ken Donaldson,Martin Bushell,Anne E Willis,Marion MacFarlane
View all publications
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Product promise

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