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AB135372

Anti-CD3 antibody [SP162]

  • RabMAb
  • Recombinant
  • Lab Essentials
  • 20ul selling size
  • What is this?

4

(8 Reviews)

|

(123 Publications)

Anti-CD3 antibody [SP162] (ab135372) is a rabbit monoclonal antibody detecting CD3 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency
- Over 50 publications

View Alternative Names

CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (AB135372)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (AB135372)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling CD3 with ab135372 at a 1/150 dilution.

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] (AB135372)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] (AB135372)

Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling CD3 with purified ab135372 at 1/200 dilution (0.60 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] (AB135372)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] (AB135372)

Flow cytometric analysis of rabbit anti-CD3 (SP162) antibody ab135372 (1/150) in Jurkat (Human T cell leukemia cell line from peripheral blood) cells (green) compared to negative control of rabbit IgG (blue).

Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] (AB135372)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] (AB135372)

Immunocytochemistry/ Immunofluorescence analysis of Jurkat (human T cell leukemia T lymphocyte) cells labeling CD3 with purified ab135372 at 1/10 (10 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (AB135372)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (AB135372)

Immunohistochemical analysis of formalin fixed paraffin embedded Human tonsil labelling CD3 with ab135372 at a dilution of 1/300. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

ab135372 anti CD3 antibody SP162 was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] (AB135372)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CD3 antibody [SP162] (AB135372)

Flow Cytometry analysis of Mouse Splenocyte cells labeling CD3 with purified ab135372 at 1/20 dilution (5.95 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] (AB135372)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD3 antibody [SP162] (AB135372)

Immunocytochemistry/ Immunofluorescence analysis of EL4.IL-2 (mouse lymphoma T lymphocyte) cells labeling CD3 with purified ab135372 at 1/10 (10 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Western blot - Anti-CD3 antibody [SP162] (AB135372)
  • WB

Unknown

Western blot - Anti-CD3 antibody [SP162] (AB135372)

All lanes:

Western blot - Anti-CD3 antibody [SP162] (ab135372) at 1/150 dilution

All lanes:

Jurkat (Human T cell leukemia cell line from peripheral blood) cell lysate

Predicted band size: 113 kDa,13 kDa,14 kDa,16 kDa,19 kDa,30 kDa,40 kDa,41 kDa,51 kDa,53 kDa,58 kDa,59 kDa,60 kDa,67 kDa,68 kDa

Observed band size: 14 kDa,60 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP162

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p>Incubate with primary antibody for 10 minutes at room temperature.</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50 - 1/200", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/150", "WB-species-notes": "<p>Incubate for 1 hour at room temperature.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50 - 1/200", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Anti-CD3 antibody [SP162] (ab135372) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P and WB.

Abcam's high quality manufacturing and validation processes ensure Anti-CD3 antibody [SP162] (ab135372) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-CD3 antibody [SP162] (ab135372) has 4 independent reviews from customers.

Anti-CD3 antibody [SP162] (ab135372) specifically detects CD3 (UniProt ID: P07766; Molecular weight: 21kDa) and is sold in 100 µL, 500 µL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone SP162 - ab245731.

Antibody clone SP162 is also available pre-conjugated to a variety of labels for your convenience - APC, HRP, Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 555, Alkaline Phosphatase (ab305633, ab305634, ab307134, ab307265, ab307335, ab308972).

Anti-CD3 antibody [SP162] (ab135372) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

CD3 is a crucial T cell co-receptor involved in activating both cytotoxic (CD8+) and helper (CD4+) T cells, playing a pivotal role in immune response. In immuno-oncology, targeting CD3 with therapeutic antibodies can enhance T cell-mediated anti-tumor activity, making it a valuable strategy in cancer treatment.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by protein A/G.
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed : 10384095, PubMed : 26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed : 38614099).
See full target information CD3E
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Publications (123)

Recent publications for all applications. Explore the full list and refine your search

NAR cancer 7:zcaf037 PubMed41064808

2025

Co-expression of CD30 and SLFN11 serves as a dual biomarker for the treatment of cutaneous T-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Takatoshi Shimauchi,Junko Murai,Manami Iwasaki,Pawit Phadungsaksawasdi,Toshiyuki Ojima,Kazuyasu Fujii,Tetsuya Honda

Theranostics 15:9580-9600 PubMed41041054

2025

Macrophage-TREM2 promotes cardiac repair by restricting the infiltration of CD8 T cells via CXCL16-CXCR6 axis after myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Zhang,Sheng Wang,Yu Ding,Tao Zheng,Jie Sheng,Yanshan Chen,Zhiyue Wang,Ximei Dai,Canbiao Wang,Long Ma,Jing Pan,Yunming Zhang,Longjiang Zhang

Stem cell research & therapy 16:507 PubMed41013769

2025

E2 pretreatment alleviates aggregation of intravenously injected mesenchymal stem cells in TBI by regulating BRG1 to affect adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Wangan Li,Xiangyu Wang,Zhiming Feng,Shiting Hua,Xiaoxiong Zou,Zelin Lai,Yingqian Cai,Yuxi Zou,Yanping Tang,Cai Lin,Guobiao Luo,Xiaolin Cai,Xiaodan Jiang

Acta neuropathologica communications 13:195 PubMed40993780

2025

A mouse model of stereotactic radiosurgery-induced neuroinflammation and blood-brain barrier compromise.

Applications

Unspecified application

Species

Unspecified reactive species

Roberto J Alcazar-Felix,Lin Cheng,Abhinav Srinath,Akash Bindal,David Tieri,Diana Vera-Cruz,Madison Yuan,Sammy Allaw,Nitha Aima Muntu,Dominic Montas,András Piffkó,Erik Pearson,Bader Ali,Peter Pytel,Issam A Awad,Sean P Polster

Scientific reports 15:30313 PubMed40830367

2025

Three-dimensional analysis of intraepidermal nerve fibres and Langerhans cells in keloids with a focus on pruritus.

Applications

Unspecified application

Species

Unspecified reactive species

Haruka Matsuzoe,Koh-Ei Toyoshima,Miki Takase,Ayako Tsuchiya,Mika Ikeda,Motoko Naitoh,Kazuya Kataoka,Tomoya Kawabata,Miho Ogawa,Naoki Morimoto,Takashi Tsuji

Haematologica : PubMed40820727

2025

Single-cell RNA sequencing reveals heterogeneity of mucosa-associated invariant T cells in donor grafts and its diagnostic relevance in gastrointestinal graft--host disease.

Applications

Unspecified application

Species

Unspecified reactive species

Mengge Gao,Kainan Zhang,Siqi Li,Huidong Guo,Yuqian Sun,Jun Kong,Tingting Han,Yingjun Chang,Xiaojun Huang,Xiaosu Zhao

JCO precision oncology 9:e2500404 PubMed40749151

2025

Mucin 16-Directed Therapy in Pediatric Sarcomas: Case Evidence of Ubamatamab Efficacy in Epithelioid Sarcoma and Its Implications for Other Sarcoma Subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Denise M Connolly,Gabriel Revon-Rivière,Rose Chami,Denise Mills,Ailish C Coblentz,Thomas S Uldrick,David A Knorr,Priscila Goncalves,Michael Dobosz,Sumreen Jalal,Sarah Cohen-Gogo,Daniel A Morgenstern

Signal transduction and targeted therapy 10:223 PubMed40670349

2025

Comprehensive genomic and transcriptomic analyses reveal prognostic stratification for esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Gao,Qiming Wang,Fangqiu Fu,Yue Zhao,Teng Yang,Xiangze Li,Yihua Sun,Hong Hu,Longfei Ma,Longsheng Miao,Xiaoyang Luo,Ting Ye,Yiliang Zhang,Yang Zhang,Ziling Huang,Hang Li,Longlong Shao,Midie Xu,Kuaile Zhao,Shiyue Zhang,Mou Zhang,Jun Wang,Chong Dai,Xiaoxiao Shang,Tingyi An,Yawei Zhang,Jiaqing Xiang,Zhiwei Cao,Bin Li,Haiquan Chen

BMC gastroenterology 25:513 PubMed40640712

2025

The prognostic value of immunoscore in the early-onset colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xinchun Wu,Sen Hou,Yingjiang Ye,Zhidong Gao

Journal of toxicologic pathology 38:199-203 PubMed40606548

2025

Spontaneous T-cell lymphoblastic lymphoma in a young ICR mouse: a case report.

Applications

Unspecified application

Species

Unspecified reactive species

Minkyoung Sung,Joo-Hee Choi,Soo-Eun Sung,Kyung-Ku Kang,Sun Hee Park,Duyeol Kim,Sijoon Lee
View all publications
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