AB215267

Anti-CD3 antibody [UCH-T1]

Lab Essentials
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(1 Publication)

Mouse Monoclonal CD3d antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing CD3D protein.

View Alternative Names

CD3d, T3D, CD3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain

2 Images

Flow Cytometry - Anti-CD3 antibody [UCH-T1] (AB215267)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD3 antibody [UCH-T1] (AB215267)

Flow cytometric analysis of the dose-dependency (D, F, H) of ab215267 binding to live human PBMCs (B). The gating strategy is shown for control puposes (A, B, C, E, G).

Western blot - Anti-CD3 antibody [UCH-T1] (AB215267)

WB

Unknown

Western blot - Anti-CD3 antibody [UCH-T1] (AB215267)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-CD3 antibody [UCH-T1] (ab215267) at 3 µg/mL

Lane 1:

Jurkat cell lysate (in RIPA buffer) at 35 µg

Lane 2:

Molt4 cell lysate (in RIPA buffer) at 35 µg

Predicted band size: 19 kDa

Observed band size: 23 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

UCH-T1

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, WB

Immunogen

Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "3-4 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

Clone UCH-T1 is considered a pan T-cell marker - it can be used for the detection of T cell populations in peripheral blood and lymph nodes and the categorisation of T versus B cell lymphomas and leukaemias. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, Sézary leukaemias and approximately 70% of acute lymphoblastic leukaemias of T cell origin.

ab215267 is a recombinant monoclonal antibody produced from the original monoclonal with variable regions (i.e. specificity) from the hybridoma.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed : 12215456).

See full target information CD3D

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

General physiology and biophysics 39:229-237 PubMed32525816

2020

The circRNA-MYLK plays oncogenic roles in the Hep-2 cell line by sponging microRNA-145-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Yao Chen,Yanmei Wang,Yanmei Li,Xuechan Li,Tiejun Yuan,Shuqin Yang,Xiaoyan Sun

View all publications

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Product promise

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