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Mouse Monoclonal CD3d antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD3D protein.
Alternative names=T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain, CD3D, T3D
IgG1
Mouse
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS
Liquid
Monoclonal
Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information. Database link: P04234
Flow Cyt | WB | |
---|---|---|
Human | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 3.00000-4.00000 µg/mL | Notes - |
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells(PubMed:12215456).
Mouse Monoclonal CD3d antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Immunogen corresponding to Cell preparation containing CD3D protein.
Alternative names=T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain, CD3D, T3D
IgG1
Mouse
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS
Liquid
Monoclonal
Cell preparation containing CD3D protein. The exact immunogen used to generate this antibody is proprietary information. Database link: P04234
UCH-T1
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Clone UCH-T1 is considered a pan T-cell marker - it can be used for the detection of T cell populations in peripheral blood and lymph nodes and the categorisation of T versus B cell lymphomas and leukaemias. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukaemia cells, Sézary leukaemias and approximately 70% of acute lymphoblastic leukaemias of T cell origin.
ab215267 is a recombinant monoclonal antibody produced from the original monoclonal with variable regions (i.e. specificity) from the hybridoma.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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