JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB174589

Anti-CD3 epsilon antibody [145-2C11] - Low endotoxin, Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Armenian hamster Monoclonal CD3E antibody. Carrier free. Suitable for IP, Flow Cyt, WB, IF, FuncS (Act), IHC-Fr and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD3e, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, Cd3e

  • 519 FITC

    FITC Anti-CD3 antibody [145-2C11]

  • 695 PerCP/Cy5.5®

    PerCP/Cy5.5® Anti-CD3 epsilon antibody [145-2C11]

  • 675 PerCP

    PerCP Anti-CD3 epsilon antibody [145-2C11]

  • 660 APC

    APC Anti-CD3 epsilon antibody [145-2C11]

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

145-2C11

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

IHC-Fr, WB, Flow Cyt, IF, IP, FuncS (Act)

applications

style
left-column

Alternative Products

Primary Antibodies

AB52959

Anti-CD3 epsilon antibody [EP449E]

RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] (AB52959)

  • 5
  • 3
Primary Antibodies

AB231830

Anti-CD3 epsilon antibody [EPR20752] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR20752] - BSA and Azide free (AB231830)

  • 0
  • 0
Primary Antibodies

AB215212

Anti-CD3 epsilon antibody [EPR20752]

RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR20752] (AB215212)

  • 0
  • 0
Primary Antibodies

AB32186

Anti-CD3 epsilon antibody [E272]

RabMAb|Recombinant|KO Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [E272] (AB32186)

  • 0
  • 0
Primary Antibodies

AB213608

Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (AB213608)

  • 0
  • 0
Primary Antibodies

AB133628

Anti-CD3 epsilon antibody [EPR5361(2)]

RabMAb|Recombinant|Lab Essentials

Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628)

  • 0
  • 0

Complementary Products

Assay Kits

AB277708

Human CD3 Epsilon ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human CD3 Epsilon ELISA Kit (AB277708)

  • 0
  • 0
Primary Antibodies

AB217344

Anti-CD8 alpha antibody [EPR21769]

RabMAb|Recombinant

Flow Cytometry - Anti-CD8 alpha antibody [EPR21769] (AB217344)

  • 5
  • 6
Primary Antibodies

AB183685

Anti-CD4 antibody [EPR19514]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR19514] (AB183685)

  • 5
  • 21
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IF" : {"fullname" : "Immunofluorescence", "shortname":"IF"}, "FuncSAct" : {"fullname" : "Functional Studies (Act)", "shortname":"FuncS (Act)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IF-species-checked": "guaranteed", "IF-species-dilution-info": "", "IF-species-notes": "<p></p>", "FuncSAct-species-checked": "guaranteed", "FuncSAct-species-dilution-info": "", "FuncSAct-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Constituents: 0.87% Sodium chloride, 0.16% Sodium phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed : 19956738, PubMed : 24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (By similarity).
See full target information Cd3e
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 27:1383-1397 PubMed31562368

2019

RBX1 prompts degradation of EXO1 to limit the homologous recombination pathway of DNA double-strand break repair in G1 phase.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Xie,Yi-Ke Liu,Zong-Pei Guo,Hua Guan,Xiao-Dan Liu,Da-Fei Xie,Yi-Guo Jiang,Teng Ma,Ping-Kun Zhou
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com