Name: Anti-CD3 epsilon antibody
SKU: ab5690
Rating: 4 (46 reviews)

Anti-CD3 epsilon antibody is a rabbit polyclonal antibody that is used to detect CD3 epsilon in IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.

- Cited in >545 publications
- Tried and trusted by researchers since 2003

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody (AB5690), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS
Form: Liquid
Clonality: Polyclonal

Immunogen

Synthetic Peptide within Human CD3E aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link P07766

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	WB
Human	Tested	Tested
Mouse	Tested	Tested
Rat	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes The recommended starting incubation time is 10min. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Human	Dilution info 1/100	Notes The recommended starting incubation time is 10min. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 0.5-2 µg/mL	Notes -
Species Rat	Dilution info 0.5-2 µg/mL	Notes -
Species Human	Dilution info 0.5-2 µg/mL	Notes -

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Target data

See full target information CD3E

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed:38614099).

Alternative names

CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS
Form: Liquid
Clonality: Polyclonal
Immunogen: Synthetic Peptide within Human CD3E aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link P07766
Purification technique: Affinity purification Immunogen
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

Product Specifications

Anti-CD3 epsilon antibody (ab5690) is a rabbit polyclonal antibody and is validated for use in IHC-P, WB in human, mouse, rat samples.
Anti-CD3 epsilon antibody (ab5690) specifically detects CD3 epsilon (UniProt ID: P07766; Molecular weight: 21kDa) and is sold in 100 µg selling sizes.

Quality and Validation

Abcam's high quality validation processes ensure Anti-CD3 epsilon antibody (ab5690) has high sensitivity and specificity.
Anti-CD3 epsilon antibody (ab5690) has been cited over 548 times in peer reviewed journals and is trusted by the scientific community.
Anti-CD3 epsilon antibody (ab5690) has 46 independent reviews from customers.

Target Information

CD3 epsilon (CD3ε) is a protein subunit of the CD3 complex, which is essential for T-cell receptor (TCR) signaling and T-cell activation. It plays a crucial role in the immune response by facilitating the recognition of antigens. CD3ε is widely used as a marker for T cells in immunological studies. Mutations or defects in CD3ε can lead to severe combined immunodeficiency (SCID), characterized by the absence of functional T cells, resulting in increased susceptibility to infections and impaired immune responses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

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11 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody (ab5690)
IHC image of CD3 staining in a formalin fixed, paraffin embedded normal human tonsil tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. The section was then incubated with ab5690 at 1/100 dilution for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Divan, A. et al LoS One. 2018 May 3;13(5):e0196893. doi: 10.1371/journal.pone.0196893. eCollection 2018 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody (ab5690)
T cells in central nervous system during late disseminated infection
A-E) Representative epifluorescence images of T cells (CD3), blood vessels (CD31), and nucleated cells (DAPI) in the brain, dura mater, and pia mater. (A) Epifluorescence images described from left to right. CD3 shown in FITC channel; CD31+ blood vessels shown in TRITC channel; nucleated cells shown in DAPI channel; merged image showing CD3+ cell associated with pia mater within the commissure of the isocortex. (B) T cells within the lymphatic-like vascular region of the sagittal sinus in the dura mater. (C) T cell associated with a blood vessel in the vasculature of the brain choroid plexus. (D) T cell associated with blood vessel in the dura mater. (E) T cell in extravascular region of the dura mater.
(After Figure 3 of Dirvan et al)
Western blot - Anti-CD3 epsilon antibody (ab5690)
Lanes 1 - 6: Merged signal (red and green). Green – ab5690 observed at 23 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab5690 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control) overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at a 1:10000 dilution for 1hr at room temperature and then imaged.
All lanes: Western blot - Anti-CD3 epsilon antibody (ab5690) at 1 µg/mL
Lane 1: THP1 whole cell lysate (-ve control) at 15 µg
Lane 2: Raji whole cell lysate (-ve control) at 15 µg
Lane 3: Jurkat whole cell lysate at 15 µg
Lane 4: Human Thymus tissue lysate at 15 µg
Lane 5: Mouse Thymus tissue lysate at 15 µg
Lane 6: Rat Thymus tissue lysate at 15 µg
Secondary
All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 19 kDa
Observed band size: 23 kDa
Western blot - Anti-CD3 epsilon antibody (ab5690)
Lanes 1 - 2: Western blot - Anti-CD3 epsilon antibody (ab5690) at 1 µg/mL
Lanes 3 - 4: No primary antibody
Lanes 1 and 3: Jurkat cell lysate at 30 µg
Lanes 2 and 4: Rat thymus tissue lysate at 20 µg
Secondary
All lanes: Goat anti-rabbit IgG (H+L), highly cross - adsorbed, HiLyte™ Fluor 750-labeled at 1/12500 dilution
Predicted band size: 19 kDa
Observed band size: 23 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody (ab5690)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD3 with ab5690 at 2μl/ml. Slides were steamed in IHC epitope retrieval solution for 35 minutes and then cooled for 20 minutes. Samples were incubated with the primary antibody at room temperature for 1 hour, incubated with a biotinylated secondary antibody for 30 minutes followed by HRP-Streptavidin for 30 minutes. Developed with DAB chromogen substrate for 5-10 minutes. Counter stained with hematoxylin. Magnification: 10X.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody (ab5690)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD3 with ab5690 at 2μl/ml. Slides were steamed in IHC epitope retrieval solution for 35 minutes and then cooled for 20 minutes. Samples were incubated with the primary antibody at room temperature for 1 hour, incubated with a biotinylated secondary antibody for 30 minutes followed by HRP-Streptavidin for 30 minutes. Developed with DAB chromogen substrate for 5-10 minutes. Counter stained with hematoxylin. Magnification: 40X.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody (ab5690)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue labelling CD3 with ab5690 at 2μl/ml. Slides were steamed in IHC epitope retrieval solution for 35 minutes and then cooled for 20 minutes. Samples were incubated with the primary antibody at room temperature for 1 hour, incubated with a biotinylated secondary antibody for 30 minutes followed by HRP-Streptavidin for 30 minutes. Developed with DAB chromogen substrate for 5-10 minutes. Counter stained with hematoxylin. Magnification: 10X.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody (ab5690)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue labelling CD3 with ab5690 at 2μl/ml. Slides were steamed in IHC epitope retrieval solution for 35 minutes and then cooled for 20 minutes. Samples were incubated with the primary antibody at room temperature for 1 hour, incubated with a biotinylated secondary antibody for 30 minutes followed by HRP-Streptavidin for 30 minutes. Developed with DAB chromogen substrate for 5-10 minutes. Counter stained with hematoxylin. Magnification: 40X.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody (ab5690)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue showing no expression of CD3 when labelled with ab5690 at 2μl/ml. Slides were steamed in IHC epitope retrieval solution for 35 minutes and then cooled for 20 minutes. Samples were incubated with the primary antibody at room temperature for 1 hour, incubated with a biotinylated secondary antibody for 30 minutes followed by HRP-Streptavidin for 30 minutes. Developed with DAB chromogen substrate for 5-10 minutes. Counter stained with hematoxylin. Magnification: 40X.
This image is courtesy of an Anonymous customer review.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody (ab5690)
ab5690 staining CD3 epsilon in human lymph node tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde and subjected to heat-mediated antigen retrieval in citric buffer pH 6.0, prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated goat anti-rabbit polyclonal was used as the secondary antibody, diluted 1/200.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-CD3 epsilon antibody (ab5690)
CD3 epsilon western blot using anti-CD3 epsilon antibody ab5690. Publication image and figure legend from Guido, M. C., Marques, A. F., et al., 2017, Oxid Med Cell Longev, PubMed 28781721.

ab5690 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab5690 please see the product overview.
(a) Total inflammatory cell count in SEN and INT areas. HE-stained sections, under 400x magnification. θp < 0.05 versus the DM SEN area; ∗p < 0.01 versus the CT SEN area, #p < 0.01 versus the DM SEN area; †p < 0.01 versus the CT INT area. Data expressed in mean ± SEM. Western blot protein expression analysis depicting CD68 (b), CD3 (c), MCP-1 (d), TNF-α (e), IL1-β (f), and IL-6 (g) was performed 8 weeks after diabetes induction. ∗p < 0.05 and †p < 0.001 versus the CT group. Data expressed in mean ± SEM in all plots.