Anti-CD3 epsilon antibody [BL-298-5D12]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Formalin-fixed, paraffin-embedded human lung carcinoma stained for CD3E using ab243873 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. Opal™ staining.

• Flow Cyt

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Flow Cytometry - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Flow cytometric analysis of Jurkat cells incubated with 1 μL ab243873 (red shaded) or isotype control (unshaded). Cells were fixed with 4% formaldehyde, and permeabilized with 90% methanol. DyLight^® 650-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Formalin-fixed, paraffin-embedded human breast carcinoma stained for CD3E using ab243873 in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD3E using ab243873 in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Formalin-fixed, paraffin-embedded human appendix stained for CD3E using ab243873 at 1/250 dilution in immunohistochemical analysis. A DyLight^® 594-conjugated goat anti-rabbit IgG was used as the secondary.

• IP

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Immunoprecipitation - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

CD3E was immunoprecipitated from 1 mg Jurkat whole cell lysate with ab243873 at 20 μL per reaction. Western blot was performed on the immunoprecipitate using ab243873 at 1/1000 dilution.

Detection : Chemiluminescence.

All lanes:

Immunoprecipitation - Anti-CD3 epsilon antibody [BL-298-5D12] (ab243873) at 1/1000 dilution

Lane 1:

ab243873 IP in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 2:

Contol IgG in Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate.

Predicted band size: 23 kDa

false

Exposure time: 3s

• WB

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Western blot - Anti-CD3 epsilon antibody [BL-298-5D12] (AB243873)

Detection : chemiluminescence

All lanes:

Western blot - Anti-CD3 epsilon antibody [BL-298-5D12] (ab243873) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 2:

K562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 50 µg

Lane 3:

SK-MEL-28 whole cell lysate at 50 µg

Lane 4:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 50 µg

Lane 5:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 23 kDa

false

Exposure time: 3s