Rabbit Recombinant Monoclonal CD3E antibody. Suitable for mIHC, IP, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications.
pH: 7.5
Preservative: 0.05% Sodium azide
Constituents: PBS
mIHC | IP | Flow Cyt (Intra) | WB | ICC/IF | IHC-P | |
---|---|---|---|---|---|---|
Human | Tested | Tested | Tested | Not recommended | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed:38614099).
CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain
Rabbit Recombinant Monoclonal CD3E antibody. Suitable for mIHC, IP, Flow Cyt (Intra), ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications.
pH: 7.5
Preservative: 0.05% Sodium azide
Constituents: PBS
Purity is greater than 99%.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Full details and terms and conditions can be found here:
Terms & Conditions.
CD3 epsilon was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab237707 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237707 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: Jurkat whole cell lysate 10 μg (Input).
Lane 2: ab237707 IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab237707 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
All lanes: Immunoprecipitation - Anti-CD3 epsilon antibody [CAL54] (ab237707)
Predicted band size: 23 kDa
Observed band size: 21 kDa
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD3 epsilon with ab237707 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the human tonsil is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab237707 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue labeling CD3 epsilon with ab237707 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the infiltrating T lymphocytes in the human gastric carcinoma is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab237707 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
CD3 epsilon was immunoprecipitated from 0.35 mg of human thymus lysate with ab237707 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237707 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: Human thymus lysate 10 μg (Input).
Lane 2: ab237707 IP in human thymus lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab237707 in human thymus lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
All lanes: Immunoprecipitation - Anti-CD3 epsilon antibody [CAL54] (ab237707)
Predicted band size: 23 kDa
Observed band size: 21 kDa
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling CD3 epsilon with ab237707 at 1/55 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in Jurkat cells. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
PBS only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
Flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween 20 permeabilized human PBMCs (peripheral blood mononuclear cells) labeling CD3 epsilon with ab237707 at 1/500 (Right compared with a Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left). Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed ab150097), at 1/5000 dilution was used as the secondary antibody.
Cells were surface stained with anti-CD3 epsilon conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min followed by intracellular staining rabbit IgG (Left) or ab237707 (Right).
Formalin-fixed, paraffin-embedded human NSCLC tissue stained for CD3 epsilon using ab237707 at 0.5 μg/mL in immunohistochemcial analysis.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD3 epsilon using ab237707 at 0.5 μg/mL in immunohistochemcial analysis.
Fluorescence multiplex immunohistochemical analysis of human tonsil (formalin-fixed paraffin-embedded section). Merged staining of Panel A: merged staining of anti-TCR beta (green; Opal™520) and anti-CD3E (red; Opal™570) on human tonsil, Panel B: anti-TCR beta staining T lymphocytes of human tonsil, Panel C: anti-CD3E staining T lymphocytes of human tonsil. DAPI was used as a counter stain. Followed by Opal Polymer HRP Ms + Rb. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining: in the order of Anti-TCR beta antibody [EPR24928-66] ab315324, ab237707 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver cancer labelling CD208 with Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573 at 1/1200 (B), CD3E with ab237707 at 1/1200 dilution (C) and CD19 with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human liver cancer.
Panel B: anti-CD208 staining dendritic cells in human liver cancer.
Panel C: anti-CD3E staining T lymphocytes in human liver cancer.
Panel D: anti-CD19 staining B lymphocytes of human liver cancer.
The section was incubated in three rounds of staining: in the order Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573, ab237707, and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon cancer labelling CD208 with Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573 at 1/1200 (B), CD3E with ab237707 at 1/1200 dilution (C) and CD19 with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human colon cancer.
Panel B: anti-CD208 staining dendritic cells in human colon cancer.
Panel C: anti-CD3E staining T lymphocytes in human colon cancer.
Panel D: anti-CD19 staining B lymphocytes of human colon cancer.
The section was incubated in three rounds of staining: in the order Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573, ab237707, and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon labelling CD208 with Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573 at 1/1200 (B), CD3E with ab237707 at 1/1200 dilution (C) and CD19 with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human colon.
Panel B: anti-CD208 staining dendritic cells in human colon.
Panel C: anti-CD3E staining T lymphocytes in human colon.
Panel D: anti-CD19 staining B lymphocytes of human colon.
The section was incubated in three rounds of staining: in the order Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573, ab237707, and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil labelling CD208 with Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573 at 1/1200 (B), CD3E with ab237707 at 1/1200 dilution (C) and CD19 with Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 at 1/1200 dilution(D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Panel A: merged staining of anti-CD208 (red; Opal™570), anti-CD3E (green; Opal™520) and anti-CD19 (magenta; Opal™690) on human tonsil.
Panel B: anti-CD208 staining dendritic cells in human tonsil.
Panel C: anti-CD3E staining T lymphocytes in human tonsil.
Panel D: anti-CD19 staining B lymphocytes of human tonsil.
The section was incubated in three rounds of staining: in the order Anti-CD208 antibody [EPR24265-8] - BSA and Azide free ab281573, ab237707, and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human tonsil tissue staining TCF7 with Anti-TCF7 antibody [EPR28579-32] ab315390 at a 1:2000 (0.26 ug/ml) dilution, ab237707 anti-CD3E used at 1:500 (1.01 ug/ml) dilution and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 anti-CD19 used at a 1:5000 (0.21 ug/ml) dilution.
Panel A: merged staining of anti-TCF7 (magenta; Opal™690), anti-CD3E (green; Opal™520) and anti-CD19 (red; Opal™570) on human tonsil.
Panel B: anti-TCF7 staining T lymphocytes of human tonsil.
Panel C: anti-CD3E staining T lymphocytes of human tonsil.
Panel D: anti-CD19 staining B lymphocytes of human tonsil.
The section was incubated in three rounds of staining: in the order of Anti-TCF7 antibody [EPR28579-32] ab315390, ab237707, and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human tonsil tissue staining TCF7 with Anti-TCF7 antibody [EPR28579-5] ab315392 at a 1:2000 (0.26 ug/ml) dilution, ab237707 anti-CD3E used at 1:500 (1.01 ug/ml) dilution and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 anti-CD19 used at a 1:5000 (0.21 ug/ml) dilution.
Panel A: merged staining of anti-TCF7 (magenta; Opal™690), anti-CD3E (green; Opal™520) and anti-CD19 (red; Opal™570) on human tonsil.
Panel B: anti-TCF7 staining T lymphocytes of human tonsil.
Panel C: anti-CD3E staining T lymphocytes of human tonsil.
Panel D: anti-CD19 staining B lymphocytes of human tonsil.
The section was incubated in three rounds of staining: in the order of Anti-TCF7 antibody [EPR28579-5] ab315392, ab237707, and Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human tonsil tissue staining CD21 with Anti-CD21 antibody [EPR27369-9] ab315160 at a 1:50 (10.56 ug/ml) dilution, Anti-CD19 antibody [SP291] - BSA and Azide free ab237772 anti-CD19 used at 1:5000 (0.21 ug/ml) dilution and ab237707 anti-CD3E used at a 1:500 (1.01 ug/ml) dilution.
Panel A: merged staining of anti-CD21 (green; Opal™690), anti-CD3E (grey; Opal™520) and anti-CD19 (red; Opal™570) on human tonsil.
Panel B: anti-CD21 staining mature B lymphocytes and T-lymphocytes of human tonsil.
Panel C: anti-CD19 staining B lymphocytes of human tonsil.
Panel D: anti-CD3E staining T lymphocytes of human tonsil.
The section was incubated in three rounds of staining: in the order of Anti-CD21 antibody [EPR27369-9] ab315160, Anti-CD19 antibody [SP291] - BSA and Azide free ab237772, and ab237707 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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