Skip to main content

Anti-CD3 epsilon antibody [CAL57] (ab237721) is a rabbit monoclonal antibody detecting CD3 epsilon in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC-Fr, mIHC. Suitable for Human, Mouse, Rat.



- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents

- Biophysical QC for unrivalled batch-batch consistency


Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL57] (AB237721), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL57] (AB237721), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-CD3 epsilon antibody [CAL57] (AB237721), expandable thumbnail
  • Western blot - Anti-CD3 epsilon antibody [CAL57] (AB237721), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CD3 epsilon antibody [CAL57] (AB237721), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.5
Preservative: 0.05% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIHC-PIPWBICC/IFIHC-FrFlow Cyt (Intra)
Human
Expected
Tested
Expected
Tested
Not recommended
Expected
Tested
Mouse
Tested
Tested
Tested
Tested
Not recommended
Tested
Tested
Rat
Tested
Tested
Expected
Tested
Not recommended
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed:38614099).

Alternative names

Recommended products

Anti-CD3 epsilon antibody [CAL57] (ab237721) is a rabbit monoclonal antibody detecting CD3 epsilon in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, IHC-Fr, mIHC. Suitable for Human, Mouse, Rat.



- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents

- Biophysical QC for unrivalled batch-batch consistency


Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
CAL57
Purification technique
Affinity purification Protein A
Concentration
Loading...
Purification notes

Purity >99%.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-CD3 epsilon antibody [CAL57] (ab237721) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Multiplex IHC (mIHC) in Human, Mouse, Rat samples.

What is the molecular weight of CD3 epsilon?


Anti-CD3 epsilon [CAL57] (ab237721) specifically detects a band for CD3 epsilon (UniProt: P22646) at a molecular weight of 23kDa.

Trusted by the scientific community


Anti-CD3 epsilon [CAL57] (ab237721) was first used in a scientific publication in 2018 and has been cited over 10 times in peer-reviewed journals.

Other related products


We have a range of other formats of antibody clone [CAL57] also available for your convenience:
ab237721, Carrier free - Anti-CD3 epsilon antibody [CAL57] - BSA and Azide free ab251607



Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

38 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD3 epsilon with ab237721 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the T lymphocytes in the human tonsil. The section was incubated with ab237721 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CD3 epsilon with ab237721 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the T lymphocytes in the human colon. The section was incubated with ab237721 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Frozen sections) - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse thymus tissue labeling CD3 epsilon with ab237721 at 1/500 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Positive staining on T cell surface (PMID: 8490660) is observed. Counterstained with DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

  • Western blot - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Western blot - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    The expression pattern is consistent with what described in the literature. (PMID: 19542373).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CD3 epsilon antibody [CAL57] (ab237721) at 1/100000 dilution

    Lane 1: EL4.IL-2 (Mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

    Lane 2: Mouse lymph node lysate at 20 µg

    Lane 3: Rat thymus lysate at 20 µg

    Lane 4: Rat spleen lysate at 20 µg

    Lane 5: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

    Predicted band size: 23 kDa

    Exposure time: 3min

  • Flow Cytometry (Intracellular) - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized mouse splenocytes labeling CD3 epsilon with ab237721 at 1/500 (right panel) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

    Cells were surface stained with anti-CD3 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min and intracellularly stained with rabbit IgG (Left) or ab237721 (Right). They recognize the same cell population.

  • Flow Cytometry (Intracellular) - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized human peripheral blood mononuclear cell (PBMC) labeling CD3 epsilon with ab237721 at 1/500 (right panel) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed ab150097), at 1/5000 dilution was used as the secondary antibody.

    Cells were surface stained with anti-CD3 conjugated to Alexa Fluor® 647. Then fixed with 2% PFA for 10min and intracellularly stained with rabbit IgG (Left) or ab237721 (Right). They recognize the same cell population.

  • Immunoprecipitation - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Immunoprecipitation - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    CD3 epsilon was immunoprecipitated from 0.35 mg EL4.IL2 (Mouse lymphoma T lymphocyte) whole cell lysate with ab237721 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab237721 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used at 1/5000 dilution.
    Lane 1: EL4.IL2 whole cell lysate 10 μg (Input).
    Lane 2: ab237721 IP in EL4.IL2 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab237721 in EL4.IL2 whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 5 seconds.

    All lanes: Immunoprecipitation - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Predicted band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD3 epsilon with ab237721 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the T lymphocytes in the rat spleen. The section was incubated with ab237721 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD3 epsilon with ab237721 at 1/2000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on the T lymphocytes in the mouse spleen. The section was incubated with ab237721 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Formalin-fixed, paraffin-embedded mouse spleen tissue stained for CD3 epsilon using ab237721 at 0.5 μg/ml in immunohistochemical analysis.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Immunohistochemistry analysis of (Formalin/PFA-fixed paraffin-embedded sections) Rat lymph node tissue labelling MAdCAM1 with Anti-MAdCAM1 antibody [EPR27223-58] ab309487 at 1:100 dilution (B), CD3 epsilon with ab237721at 1:2000 dilution (C) and CD20 with Anti-CD20 antibody [SP32] ab64088 at 1:100 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Panel A: merged staining of anti-MAdCAM (green; Opal™520), anti-CD3 (gray; Opal™690) and anti-CD20 (magenta; Opal™570) on rat lymph node.

    Panel B: anti-MAdCAM staining high endothelial venules in rat lymph node.

    Panel C: ant-CD3 staining B lymphocytes in rat lymph node.

    Panel D: ant-CD20 staining B lymphocytes in rat lymph node.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-MAdCAM1 antibody [EPR27223-58] ab309487, ab237721 and Anti-CD20 antibody [SP32] ab64088 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Immunohistochemistry analysis of (Formalin/PFA-fixed paraffin-embedded sections) Mouse lymph node tissue labelling MAdCAM1 with Anti-MAdCAM1 antibody [EPR27223-58] ab309487 at 1:100 dilution (B), CD3 epsilon with ab237721at 1:2000 dilution (C) and CD20 with Anti-CD20 antibody [SP32] ab64088 at 1:100 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Panel A: merged staining of anti-MAdCAM (green; Opal™520), anti-CD3 (gray; Opal™690) and anti-CD20 (magenta; Opal™570) on mouse lymph node.

    Panel B: anti-MAdCAM staining high endothelial venules in mouse lymph node.

    Panel C: ant-CD3 staining B lymphocytes in mouse lymph node.

    Panel D: ant-CD20 staining B lymphocytes in mouse lymph node.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-MAdCAM1 antibody [EPR27223-58] ab309487, ab237721 and Anti-CD20 antibody [SP32] ab64088 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse small intestine tissue staining CD68 with Anti-CD68 antibody [EPR23917-164] ab283654 at a 1/100 dilution, Anti-CD19 antibody [EPR23174-145] ab245235 anti-CD19 used at 1/1000 dilution and ab237721 anti-CD3 used at a 1/2000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Panel A: merged staining of anti-CD68 (magenta; Opal520), anti-CD19 (green; Opal690) and anti-CD3 (grey; Opal570) on mouse small intestine.

    Panel B: anti-CD68 staining macrophages in mouse small intestine.

    Panel C: anti-CD19 staining B lymphocytes in mouse small intestine.

    Panel D: anti-CD3 staining T lymphocytes in mouse small intestine.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-CD68 antibody [EPR23917-164] ab283654, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND®RX instrument with an Opal 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse colon tissue staining CD68 with Anti-CD68 antibody [EPR23917-164] ab283654 at a 1/100 dilution, Anti-CD19 antibody [EPR23174-145] ab245235 anti-CD19 used at 1/1000 dilution and ab237721 anti-CD3 used at a 1/2000 dilution. Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Panel A: merged staining of anti-CD68 (magenta; Opal520), anti-CD19 (green; Opal690) and anti-CD3 (grey; Opal570) on mouse colon.

    Panel B: anti-CD68 staining macrophages in mouse colon.

    Panel C: anti-CD19 staining B lymphocytes in mouse colon.

    Panel D: anti-CD3 staining T lymphocytes in mouse colon.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-CD68 antibody [EPR23917-164] ab283654, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND®RX instrument with an Opal 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spleen tissue staining CD23 with Anti-CD23 antibody [EPR28712-26] ab315289 at a 1/2000 dilution (0.253 µg/ml), CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at 1/1000 dilution (0.444 µg/ml), and CD3 with ab237721 at 1/8000 dilution (0.07 µg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

    Panel A: merged staining of anti-CD23 (green; Opal™520), anti-CD19 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on mouse spleen.
    Panel B: anti-CD23 staining B lymphocytes in mouse spleen.
    Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
    Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-CD23 antibody [EPR28712-26] ab315289, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lymph node tissue staining CD23 with Anti-CD23 antibody [EPR28712-26] ab315289 at a 1/2000 dilution (0.253 µg/ml), CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at 1/1000 dilution (0.444 µg/ml), and CD3 with ab237721 at 1/8000 dilution (0.07 µg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

    Panel A: merged staining of anti-CD23 (green; Opal™520), anti-CD19 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on mouse lymph node.
    Panel B: anti-CD23 staining B lymphocytes in mouse lymph node.
    Panel C: anti-CD19 staining B lymphocytes in mouse lymph node.
    Panel D: anti-CD3 staining T lymphocytes in mouse lymph node.
    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-CD23 antibody [EPR28712-26] ab315289, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse thymus tissue staining CD23 with Anti-CD23 antibody [EPR28712-26] ab315289 at a 1/2000 dilution (0.253 µg/ml), CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at 1/1000 dilution (0.444 µg/ml), and CD3 with ab237721 at 1/8000 dilution (0.07 µg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

    Panel A: merged staining of anti-CD23 (green; Opal™520), anti-CD19 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on mouse thymus.
    Panel B: anti-CD23 staining B lymphocytes in mouse thymus.
    Panel C: anti-CD19 staining B lymphocytes in mouse thymus.
    Panel D: anti-CD3 staining T lymphocytes in mouse thymus.
    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-CD23 antibody [EPR28712-26] ab315289, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse bone marrow staining of F4/80 with Anti-F4/80 antibody [EPR26545-166] ab300421 at a 1/5000 (0.1 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with ab237721 at 1/2000 ( 0.264 µg/ml) dilution.

    Panel A: merged staining of anti-F4/80 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse bone marrow.
    Panel B: anti-F4/80 staining macrophages in mouse bone marrow.
    Panel C: anti-CD19 staining B lymphocytes in mouse bone marrow.
    Panel D: anti-CD3 staining T lymphocytes in mouse bone marrow.
    Nuclear DNA was labelled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-F4/80 antibody [EPR26545-166] ab300421, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse thymus staining of NCR1 with Anti-NCR1 antibody [EPR23097-35] ab233558 at a 1/500 (1.062 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with ab237721 at 1/2000 ( 0.264 µg/ml) dilution.

    Panel A: merged staining of anti-NCR1 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse thymus.
    Panel B: anti-NCR1 staining natural killer cells in mouse thymus.
    Panel C: anti-CD19 staining B lymphocytes in mouse thymus.
    Panel D: anti-CD3 staining T lymphocytes in mouse thymus.
    Nuclear DNA was labelled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-NCR1 antibody [EPR23097-35] ab233558, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    CD3 epsilon Multiplex immunohistochemistry staining of Human Hodgkin's lymphoma using rabbit Anti-CD3 epsilon antibody

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human hodgkin's lymphoma tissue staining CD72 with Anti-CD72 antibody [EPR29739-578] ab324351 at a 1:500 (1.018 ug/ml) dilution, Anti-CD19 antibody [SP291] - C-terminal ab227688 anti-CD19 used at 1:100 (0.15 ug/ml) dilution and ab237721 anti-CD3 used at a 1:2000 (0.26 ug/ml) dilution.

    Panel A: merged staining of anti-CD72 (green; Opal™520), anti-CD19 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on human Hodgkin's lymphoma.

    Panel B: anti-CD72 staining immune cells in human Hodgkin's lymphoma.

    Panel C: ant-CD19 staining B lymphocytes in human Hodgkin's lymphoma.

    Panel D: ant-CD3 staining T lymphocytes in human Hodgkin's lymphoma.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-CD72 antibody [EPR29739-578] ab324351, Anti-CD19 antibody [SP291] - C-terminal ab227688 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse spleen staining of NCR1 with Anti-NCR1 antibody [EPR23097-35] ab233558 at a 1/500 (1.062 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with ab237721 at 1/2000 ( 0.264 µg/ml) dilution.

    Panel A: merged staining of anti-NCR1 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse spleen.
    Panel B: Anti-NCR1 staining natural killer cells in mouse spleen.
    Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
    Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
    Nuclear DNA was labelled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-NCR1 antibody [EPR23097-35] ab233558, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse spleen staining of F4/80 with Anti-F4/80 antibody [EPR26545-166] ab300421 at a 1/5000 (0.1 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with ab237721 at 1/2000 ( 0.264 µg/ml) dilution.

    Panel A: merged staining of anti-F4/80 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse spleen.
    Panel B: anti-F4/80 staining macrophages in mouse spleen.
    Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
    Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
    Nuclear DNA was labelled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-F4/80 antibody [EPR26545-166] ab300421, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse lymph node staining of F4/80 with Anti-F4/80 antibody [EPR26545-166] ab300421 at a 1/5000 (0.1 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with ab237721 at 1/2000 ( 0.264 µg/ml) dilution.

    Panel A: merged staining of anti-F4/80 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse lymph node.
    Panel B: anti-F4/80 staining macrophages in mouse lymph node.
    Panel C: anti-CD19 staining B lymphocytes in mouse lymph node.
    Panel D: anti-CD3 staining T lymphocytes in mouse lymph node.
    Nuclear DNA was labelled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-F4/80 antibody [EPR26545-166] ab300421, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse thymus staining of F4/80 with Anti-F4/80 antibody [EPR26545-166] ab300421 at a 1/5000 (0.1 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with ab237721 at 1/2000 ( 0.264 µg/ml) dilution.

    Panel A: merged staining of anti-F4/80 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse thymus.
    Panel B: anti-F4/80 staining macrophages in mouse thymus.
    Panel C: anti-CD19 staining B lymphocytes in mouse thymus.
    Panel D: anti-CD3 staining T lymphocytes in mouse thymus.
    Nuclear DNA was labelled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-F4/80 antibody [EPR26545-166] ab300421, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse spleen tissue staining Neutrophil Elastase with Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335 at a 1/500 ( 1.076 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at a 1/1000 ( 0.444 µg/ml) dilution and CD3 with ab237721 at a 1/2000 ( 0.264 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

    Panel A: merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B: anti-Neutrophil Elastase staining neutrophils in mouse spleen.
    Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
    Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse thymus tissue staining Neutrophil Elastase with Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335 at a 1/500 ( 1.076 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at a 1/1000 ( 0.444 µg/ml) dilution and CD3 with ab237721 at a 1/2000 ( 0.264 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

    Panel A: merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B: anti-Neutrophil Elastase staining neutrophils in mouse spleen.
    Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
    Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse bone marrow tissue staining Neutrophil Elastase with Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335 at a 1/500 ( 1.076 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at a 1/1000 ( 0.444 µg/ml) dilution and CD3 with ab237721 at a 1/2000 ( 0.264 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

    Panel A: merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B: anti-Neutrophil Elastase staining neutrophils in mouse spleen.
    Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
    Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse lymph node tissue staining Neutrophil Elastase with Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335 at a 1/500 ( 1.076 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at a 1/1000 ( 0.444 µg/ml) dilution and CD3 with ab237721 at a 1/2000 ( 0.264 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

    Panel A: merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B: anti-Neutrophil Elastase staining neutrophils in mouse spleen.
    Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
    Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse lymph node staining of NCR1 with Anti-NCR1 antibody [EPR23097-35] ab233558 at a 1/500 (1.062 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with ab237721 at 1/2000 ( 0.264 µg/ml) dilution.

    Panel A: merged staining of anti-NCR1 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse lymph node.
    Panel B: anti-NCR1 staining natural killer cells in mouse lymph node.
    Panel C: anti-CD19 staining B lymphocytes in mouse lymph node..
    Panel D: anti-CD3 staining T lymphocytes in mouse lymph node.
    Nuclear DNA was labelled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-NCR1 antibody [EPR23097-35] ab233558, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    CD3 epsilon Multiplex immunohistochemistry staining of Mouse liver using rabbit Anti-CD3 epsilon antibody

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining CD5L/CT-2 with Anti-CD5L/CT-2 antibody [EPR29364-21] ab324193 at a 1:500 (0.958 ug/ml) dilution, Anti-CD68 antibody [EPR23917-164] ab283654 anti-CD68 used at 1:100 (4.43 ug/ml) dilution and ab237721 anti-CD3 used at a 1:2000 (0.25 ug/ml) dilution.

    Panel A: merged staining of anti-CD5 antigen-like (green; Opal™520), anti-CD68 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on mouse liver.

    Panel B: anti-CD5 antigen-like staining macrophages in mouse liver.

    Panel C: anti-CD68 staining macrophages in mouse liver.

    Panel D: anti-CD3 staining T lymphocytes in mouse liver.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-CD5L/CT-2 antibody [EPR29364-21] ab324193, Anti-CD68 antibody [EPR23917-164] ab283654 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    CD3 epsilon Multiplex immunohistochemistry staining of human liver tissue using rabbit Anti-CD3 epsilon antibody

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining MARCO with Anti-MARCO antibody [RM1219] ab323717 at a 1:200 (2.49 ug/ml) dilution, Anti-CD68 antibody [EPR23917-164] ab283654 anti-CD68 used at 1:500 (1.26 ug/ml) dilution and ab237721 anti-CD3 used at a 1:2000 (0.26 ug/ml) dilution.

    Panel A: merged staining of anti-MARCO (green; Opal™520), anti-CD68 (magenta; Opal™570) and anti-CD3 (yellow; Opal™690) on human liver.

    Panel B: anti-MARCO staining macrophages in human liver.

    Panel C: ant-CD68 staining macrophages in human liver.

    Panel D: ant-CD3 staining T lymphocytes in human liver.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-MARCO antibody [RM1219] ab323717, Anti-CD68 antibody [EPR20545] ab213363 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    CD3 epsilon Multiplex immunohistochemistry staining of rat liver tissue using rabbit Anti-CD3 epsilon antibody

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining MARCO with Anti-MARCO antibody [RM1219] ab323717 at a 1:200 (2.49 ug/ml) dilution, Anti-CD68 antibody [EPR23917-164] ab283654 anti-CD68 used at 1:100 (4.43 ug/ml) dilution and ab237721 anti-CD3 used at a 1:2000 (0.26 ug/ml) dilution.

    Panel A: merged staining of anti-MARCO (green; Opal™520), anti-CD68 (magenta; Opal™690) and anti-CD3 (yellow; Opal™570) on rat liver.

    Panel B: anti-MARCO staining macrophages in rat liver.

    Panel C: ant-CD68 staining macrophages in rat liver.

    Panel D: ant-CD3 staining T lymphocytes in rat liver.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-MARCO antibody [RM1219] ab323717, Anti-CD68 antibody [EPR23917-164] ab283654 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    CD3 epsilon Multiplex immunohistochemistry staining of mouse lung tissue using rabbit Anti-CD3 epsilon antibody

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lung tissue staining MARCO with Anti-MARCO antibody [RM1219] ab323717 at a 1:200 (2.49 ug/ml) dilution, Anti-CD68 antibody [EPR23917-164] ab283654 anti-CD68 used at 1:100 (4.43 ug/ml) dilution and ab237721 anti-CD3 used at a 1:2000 (0.26 ug/ml) dilution.

    Panel A: merged staining of anti-MARCO (green; Opal™520), anti-CD68 (magenta; Opal™690) and anti-CD3 (yellow; Opal™570) on mouse lung.

    Panel B: anti-MARCO staining macrophages in mouse lung.

    Panel C: ant-CD68 staining macrophages in mouse lung.

    Panel D: ant-CD3 staining T lymphocytes in mouse lung.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-MARCO antibody [RM1219] ab323717, Anti-CD68 antibody [EPR23917-164] ab283654 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    CD3 epsilon Multiplex immunohistochemistry staining of human liver tissue using rabbit Anti-CD3 epsilon antibody

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human liver tissue staining MARCO with Anti-MARCO antibody [RM1219] ab323717 at a 1:200 (2.49 ug/ml) dilution, Anti-CD19 antibody [EPR28949-559] ab320735 anti-CD19 used at 1:2000 (0.255 ug/ml) dilution and ab237721 anti-CD3 used at a 1:2000 (0.26 ug/ml) dilution.

    Panel A: merged staining of anti-MARCO (green; Opal™520), anti-CD19 (gray; Opal™570) and anti-CD3 (magenta; Opal™690) on human liver.

    Panel B: anti-MARCO staining macrophages in human liver.

    Panel C: ant-CD19 staining B lymphocytes in human liver.

    Panel D: ant-CD3 staining T lymphocytes in human liver.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-MARCO antibody [RM1219] ab323717, Anti-CD19 antibody [EPR28949-559] ab320735 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    CD3 epsilon Multiplex immunohistochemistry staining of rat liver tissue using rabbit Anti-CD3 epsilon antibody

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat liver tissue staining MARCO with Anti-MARCO antibody [RM1219] ab323717 at a 1:200 (2.49 ug/ml) dilution, Anti-CD20 antibody [SP32] ab64088 anti-CD20 used at 1:100 (2.91 ug/ml) dilution and ab237721 anti-CD3 used at a 1:2000 (0.26 ug/ml) dilution.

    Panel A: merged staining of anti-MARCO (green; Opal™520), anti-CD20 (gray; Opal™690) and anti-CD3 (magenta; Opal™570) on rat liver.

    Panel B: anti-MARCO staining macrophages in rat liver.

    Panel C: ant-CD20 staining B lymphocytes in rat liver.

    Panel D: ant-CD3 staining T lymphocytes in rat liver.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-MARCO antibody [RM1219] ab323717, Anti-CD20 antibody [SP32] ab64088 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    CD3 epsilon Multiplex immunohistochemistry staining of mouse liver tissue using rabbit Anti-CD3 epsilon antibody

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse liver tissue staining MARCO with Anti-MARCO antibody [RM1219] ab323717 at a 1:200 (2.49 ug/ml) dilution, Anti-CD19 antibody [EPR23174-145] ab245235 anti-CD19 used at 1:1000 (0.22 ug/ml) dilution and ab237721 anti-CD3 used at a 1:2000 (0.26 ug/ml) dilution.

    Panel A: merged staining of anti-MARCO (green; Opal™520), anti-CD19 (gray; Opal™690) and anti-CD3 (magenta; Opal™570) on mouse liver.

    Panel B: anti-MARCO staining macrophages in mouse liver.

    Panel C: ant-CD19 staining B lymphocytes in mouse liver.

    Panel D: ant-CD3 staining T lymphocytes in mouse liver.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-MARCO antibody [RM1219] ab323717, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    CD3 epsilon Multiplex immunohistochemistry staining of mouse lung tissue using rabbit Anti-CD3 epsilon antibody

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lung tissue staining MARCO with Anti-MARCO antibody [RM1219] ab323717 at a 1:200 (2.49 ug/ml) dilution, Anti-CD19 antibody [EPR23174-145] ab245235 anti-CD19 used at 1:1000 (0.22 ug/ml) dilution and ab237721 anti-CD3 used at a 1:2000 (0.26 ug/ml) dilution.

    Panel A: merged staining of anti-MARCO (green; Opal™520), anti-CD19 (gray; Opal™690) and anti-CD3 (magenta; Opal™570) on mouse lung.

    Panel B: anti-MARCO staining macrophages in mouse lung.

    Panel C: ant-CD19 staining B lymphocytes in mouse lung.

    Panel D: ant-CD3 staining T lymphocytes in mouse lung.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-MARCO antibody [RM1219] ab323717, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721), expandable thumbnail

    Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [CAL57] (ab237721)

    CD3 epsilon Multiplex immunohistochemistry staining of mouse spleen tissue using rabbit Anti-CD3 epsilon antibody

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spleen tissue staining MARCO with Anti-MARCO antibody [RM1219] ab323717 at a 1:200 (2.49 ug/ml) dilution, Anti-CD19 antibody [EPR23174-145] ab245235 anti-CD19 used at 1:1000 (0.22 ug/ml) dilution and ab237721 anti-CD3 used at a 1:2000 (0.26 ug/ml) dilution.

    Panel A: merged staining of anti-MARCO (green; Opal™520), anti-CD19 (gray; Opal™690) and anti-CD3 (magenta; Opal™570) on mouse spleen.

    Panel B: anti-MARCO staining macrophages in mouse spleen.

    Panel C: ant-CD19 staining B lymphocytes in mouse spleen.

    Panel D: ant-CD3 staining T lymphocytes in mouse spleen.

    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of Anti-MARCO antibody [RM1219] ab323717, Anti-CD19 antibody [EPR23174-145] ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Nuclear counter stain with DAPI.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com