Anti-CD3 epsilon [EP449E] antibody (ab271850) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect CD3 epsilon in Western Blot, IP, IHC-P. Suitable for Human samples.
- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (AB271850), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-bsa-and-azide-free-ab271850--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137303.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (AB271850)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (AB271850), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-bsa-and-azide-free-ab271850--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137548.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (AB271850)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (AB271850), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-bsa-and-azide-free-ab271850--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105287.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (AB271850)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (AB271850), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-bsa-and-azide-free-ab271850--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134974.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (AB271850)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (AB271850), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-bsa-and-azide-free-ab271850--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133419.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (AB271850)")
Publications
Filter by
- Cell genomics 3:1002722023The molecular consequences of androgen activity in the human breast.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFlorian Raths,Mehran Karimzadeh,Nathan Ing,Andrew Martinez,Yoona Yang,Ying Qu,Tian-Yu Lee,Brianna Mulligan,Suzanne Devkota,Wayne T Tilley,Theresa E Hickey,Bo Wang,Armando E Giuliano,Shikha Bose,Hani Goodarzi,Edward C Ray,Xiaojiang Cui,Simon R V KnottPubMed 36950379
- Cell reports 42:1119372023Single-cell RNA sequencing identifies a population of human liver-type ILC1s.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenjamin Krämer,Ansel P Nalin,Feiyang Ma,Sarah Eickhoff,Philipp Lutz,Sonia Leonardelli,Felix Goeser,Claudia Finnemann,Gudrun Hack,Jan Raabe,Michael ToVinh,Sarah Ahmad,Christoph Hoffmeister,Kim M Kaiser,Steffen Manekeller,Vittorio Branchi,Tobias Bald,Michael Hölzel,Robert Hüneburg,Hans Dieter Nischalke,Alexander Semaan,Bettina Langhans,Dominik J Kaczmarek,Brooke Benner,Matthew R Lordo,Jesse Kowalski,Adam Gerhardt,Jörg Timm,Marieta Toma,Raphael Mohr,Andreas Türler,Arthur Charpentier,Tobias van Bremen,Georg Feldmann,Arne Sattler,Katja Kotsch,Ali T Abdallah,Christian P Strassburg,Ulrich Spengler,William E Carson,Bethany L Mundy-Bosse,Matteo Pellegrini,Timothy E O'Sullivan,Aharon G Freud,Jacob NattermannPubMed 36640314
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed:38614099).
Alternative names
CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain
Recommended products
Anti-CD3 epsilon [EP449E] antibody (ab271850) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect CD3 epsilon in Western Blot, IP, IHC-P. Suitable for Human samples.
- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP449E
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human samples.
What is the molecular weight of CD3 epsilon?
Anti-CD3 epsilon [EP449E] - BSA and Azide free (ab271850) specifically detects a band for CD3 epsilon (UniProt: P07766) at a molecular weight of 23kDa.
Other related products
We have a range of other formats of antibody clone [EP449E] also available for your convenience:
Anti-CD3 epsilon antibody [EP449E] ab52959, Carrier free - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free ab213608, Carrier free - ab271850, Carrier free - ab307491
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)
Anti-CD3 epsilon antibody [EP449E] ab52959 at 1/250-1/500 dilution staining CD3 epsilon in human spleen by Immunohistochemistry, Paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 epsilon antibody [EP449E] ab52959).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-bsa-and-azide-free-ab271850--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137548.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)
Anti-CD3 epsilon antibody [EP449E] ab52959 showing positive staining in Normal tonsil tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 epsilon antibody [EP449E] ab52959).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-bsa-and-azide-free-ab271850--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105287.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)
Anti-CD3 epsilon antibody [EP449E] ab52959 showing positive staining in Normal spleen tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 epsilon antibody [EP449E] ab52959).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-bsa-and-azide-free-ab271850--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134974.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)
Anti-CD3 epsilon antibody [EP449E] ab52959 showing negative staining in Normal brain tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 epsilon antibody [EP449E] ab52959).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-bsa-and-azide-free-ab271850--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133419.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)
Anti-CD3 epsilon antibody [EP449E] ab52959 showing negative staining in Normal kidney tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 epsilon antibody [EP449E] ab52959).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-bsa-and-azide-free-ab271850--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135223.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)
Anti-CD3 epsilon antibody [EP449E] ab52959 showing negative staining in Skeletal muscle tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 epsilon antibody [EP449E] ab52959).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-bsa-and-azide-free-ab271850--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425260.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)
Immunohistochemical analysis of formalin fixed paraffin embedded human kidney labelling CD3e with ab271850 at a concentration of 5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32min with ULTRA cell conditioning solution (CC1 pH8.5). ab271850 anti CD3e antibody was incubated at 37oC for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-bsa-and-azide-free-ab271850--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135223.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-bsa-and-azide-free-ab271850--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425260.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - BSA and Azide free (ab271850)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com