Rabbit Recombinant Monoclonal CD3E antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 3 publications.
Images
![Immunoprecipitation - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (AB213608), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-low-endotoxin-azide-free-ab213608--immunoprecipitation-img3558.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (AB213608)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (AB213608), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-low-endotoxin-azide-free-ab213608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91453.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (AB213608)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (AB213608), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-low-endotoxin-azide-free-ab213608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137548.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (AB213608)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (AB213608), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-low-endotoxin-azide-free-ab213608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105287.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (AB213608)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (AB213608), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-low-endotoxin-azide-free-ab213608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134974.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (AB213608)")
Publications
Filter by
- Cell reports. Medicine 3:1005252022An omic and multidimensional spatial atlas from serial biopsies of an evolving metastatic breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrett E Johnson,Allison L Creason,Jayne M Stommel,Jamie M Keck,Swapnil Parmar,Courtney B Betts,Aurora Blucher,Christopher Boniface,Elmar Bucher,Erik Burlingame,Todd Camp,Koei Chin,Jennifer Eng,Joseph Estabrook,Heidi S Feiler,Michael B Heskett,Zhi Hu,Annette Kolodzie,Ben L Kong,Marilyne Labrie,Jinho Lee,Patrick Leyshock,Souraya Mitri,Janice Patterson,Jessica L Riesterer,Shamilene Sivagnanam,Julia Somers,Damir Sudar,Guillaume Thibault,Benjamin R Weeder,Christina Zheng,Xiaolin Nan,Reid F Thompson,Laura M Heiser,Paul T Spellman,George Thomas,Emek Demir,Young Hwan Chang,Lisa M Coussens,Alexander R Guimaraes,Christopher Corless,Jeremy Goecks,Raymond Bergan,Zahi Mitri,Gordon B Mills,Joe W GrayPubMed 35243422
- Nature communications 12:72922021Epigenetic loss of heterogeneity from low to high grade localized prostate tumours.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSebnem Ece Eksi,Alex Chitsazan,Zeynep Sayar,George V Thomas,Andrew J Fields,Ryan P Kopp,Paul T Spellman,Andrew C AdeyPubMed 34911933
- Science advances 6:eaay82712020Ultraprecise single-molecule localization microscopy enables in situ distance measurements in intact cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimao Coelho et. al.PubMed 32494604
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
1 product for Alternative Version
Target data
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed:38614099).
Alternative names
CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain
Recommended products
Rabbit Recombinant Monoclonal CD3E antibody. Carrier free. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EP449E
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody reacts with CD3 epsilon. It does not cross-react with other subunits of CD3.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab213608 is the carrier-free version of Anti-CD3 epsilon antibody [EP449E] ab52959.
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunoprecipitation - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-low-endotoxin-azide-free-ab213608--immunoprecipitation-img3558.jpg "Immunoprecipitation - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)")
Immunoprecipitation - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)
This data was developed using Anti-CD3 epsilon antibody [EP449E] ab52959, the same antibody clone in a different buffer formulation.
Purified Anti-CD3 epsilon antibody [EP449E] ab52959 at 1/20 dilution (1μg) immunoprecipitating CD3 epsilon in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10μg
Lane 2 (+): Anti-CD3 epsilon antibody [EP449E] ab52959 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab52929 in Jurkat whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 23 kDaAll lanes: Immunoprecipitation - Anti-CD3 epsilon antibody [EP449E] (Anti-CD3 epsilon antibody [EP449E] ab52959)
Predicted band size: 23 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-low-endotoxin-azide-free-ab213608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91453.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)
Anti-CD3 epsilon antibody [EP449E] ab52959 at 1/250-1/500 dilution staining CD3 epsilon in human spleen by Immunohistochemistry, Paraffin embedded tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 epsilon antibody [EP449E] ab52959).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-low-endotoxin-azide-free-ab213608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137548.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)
Anti-CD3 epsilon antibody [EP449E] ab52959 showing positive staining in Normal tonsil tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 epsilon antibody [EP449E] ab52959).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-low-endotoxin-azide-free-ab213608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105287.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)
Anti-CD3 epsilon antibody [EP449E] ab52959 showing positive staining in Normal spleen tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 epsilon antibody [EP449E] ab52959).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-low-endotoxin-azide-free-ab213608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134974.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)
Anti-CD3 epsilon antibody [EP449E] ab52959 showing negative staining in Normal brain tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 epsilon antibody [EP449E] ab52959).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-low-endotoxin-azide-free-ab213608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133419.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)
Anti-CD3 epsilon antibody [EP449E] ab52959 showing negative staining in Normal kidney tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 epsilon antibody [EP449E] ab52959).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-low-endotoxin-azide-free-ab213608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135223.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)
Anti-CD3 epsilon antibody [EP449E] ab52959 showing negative staining in Skeletal muscle tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 epsilon antibody [EP449E] ab52959).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-low-endotoxin-azide-free-ab213608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133419.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-ep449e-low-endotoxin-azide-free-ab213608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135223.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EP449E] - Low endotoxin, Azide free (ab213608)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com