Anti-CD3 epsilon antibody [EPR20752] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR20752] - BSA and Azide free (AB231830)

Immunohistochemical analysis of Paraffin-embedded sections mouse spleen tissue labeling CD3 epsilon with ab215212 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Mainly membranous staining on T cells in mouse spleen is observed (PMID : 18648532). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215212).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR20752] - BSA and Azide free (AB231830)

Immunohistochemical analysis of Paraffin-embedded sections mouse thymus tissue labeling CD3 epsilon with ab215212 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Mainly membranous staining on T cells in mouse thymus is observed (PMID : 18648532). Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215212).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CD3 epsilon antibody [EPR20752] - BSA and Azide free (AB231830)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilised mouse spleen tissue labelling CD3 epsilon with ab215212 at 1/800 dilution, foloowed by AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Positive membrane staining on cells localized in the periarterial lymphatic sheath in mouse spleen tissue section is observed (PMID : 20880086) Counterstained with DAPI.
Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215212).

• IP

Supplier Data

Immunoprecipitation - Anti-CD3 epsilon antibody [EPR20752] - BSA and Azide free (AB231830)

CD3 epsilon was immunoprecipitated from 10 μg of EL4.IL-2 (Mouse lymphoma T lymphocyte) whole cell lysate with ab215212 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215212 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1 : EL4.IL-2 (Mouse lymphoma T lymphocyte) whole cell lysate 10 μg (input).
Lane 2 : ab215212 IP in EL4.IL-2 whole cell lysate (+)
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab215212 in EL4.IL-2 whole cell lysate (-).
Blocking and dilution buffer and concentration : 5% NFDM/TBST
Exposure time : 3 minutes

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab215212).

All lanes:

Immunoprecipitation - Anti-CD3 epsilon antibody [EPR20752] (<a href='/en-us/products/primary-antibodies/cd3-epsilon-antibody-epr20752-ab215212'>ab215212</a>)

Predicted band size: 23 kDa

Observed band size: 21 kDa

false