AB231775

Anti-CD3 epsilon antibody [EPR22667-12]

RabMAb
Recombinant
Lab Essentials
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(12 Publications)

Rabbit Recombinant Monoclonal CD3E antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse samples. Cited in 12 publications.

View Alternative Names

CD3e, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, Cd3e

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD3 epsilon with ab231775 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the mouse spleen (PMID : 18648532) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized EL4.IL2 (mouse lymphoma T lymphocyte cell line) cells labeling CD3 epsilon with ab231775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in EL4.IL2 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CD3 epsilon with ab231775 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the T lymphocytes on mouse lung (PMID : 22888136) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Unknown

Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)

CD3 epsilon was immunoprecipitated from 0.35 mg of EL4.IL2 (mouse lymphoma T lymphocyte cell line) whole cell lysate with ab231775 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab231775 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as the secondary antibody at 1/5000 dilution.

Lane 1 : EL4.IL2 whole cell lysate 10 μg (Input).
Lane 2 : ab231775 IP in EL4.IL2 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab231775 in EL4.IL2 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775)

Predicted band size: 23 kDa

Observed band size: 22 kDa

false

Unknown

Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)

Blocking and dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 1387664).

All lanes:

Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775) at 1/1000 dilution

All lanes:

Mouse thymus lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 18 kDa,22 kDa

false

Exposure time: 15s

Unknown

Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775) at 1/1000 dilution

All lanes:

EL4.IL2 (mouse lymphoma T lymphocyte cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 23 kDa

Observed band size: 22 kDa

false

Exposure time: 158s

Carrier free

Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22667-12

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IP, WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed : 19956738, PubMed : 24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (By similarity).

See full target information Cd3e

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Molecular neurodegeneration 20:84 PubMed40702549

2025

Endothelium-specific endoglin triggers astrocyte reactivity via extracellular vesicles in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Pingao Zhang,Chenghuan Song,Jiyun Shi,Zijie Wei,Jing Wang,Wanying Huang,Rui Zhang,Jintao Wang,Xiaoli Yang,Gang Wang,Xiaoling Gao,Yongfang Zhang,Hongzhuan Chen,Hao Wang

Acta pharmaceutica Sinica. B 14:5479-5492 PubMed39807328

2025

Controlling tumor progression and recurrence in mice through combined treatment with a PD-L1 inhibitor and a designer strain that delivers GM-CSF.

Applications

Unspecified application

Species

Unspecified reactive species

Heung Jin Jeon,Daejin Lim,EunA So,Solbi Kim,Jae-Ho Jeong,Miryoung Song,Hyo-Jin Lee

Cell biology and toxicology 40:87 PubMed39384651

2024

Mechanisms of HIF1A-mediated immune evasion in gastric cancer and the impact on therapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Qi,Xiaoyu Ma,Yu Ma,Liuyu Jia,Kuncong Liu,Honghu Wang

Cell death & disease 15:640 PubMed39251573

2024

Mesenchymal stromal cells restrain the Th17 cell response via L-amino-acid oxidase within lymph nodes.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Ni,Le Zhen,Zhu Zeng,Jingwen Yang,Yukai Wang,Huanke Xu,Qixiang Zhang,Yongcheng Zhu,Yu Tao,Jing Wang,Qing Liu,Kezheng Yi,Yang Chen,Qian Chen,Guangji Wang,Fang Zhou,Yunlong Shan

Science advances 10:eadk9996 PubMed38838152

2024

Chemical conjugation mitigates immunotoxicity of chemotherapy via reducing receptor-mediated drug leakage from lipid nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Zheng,Wen Zhang,Xiang Gong,Fengqin Xiong,Linyang Jiang,Lingli Zhou,Yuan Zhang,Helen He Zhu,Hao Wang,Yaping Li,Pengcheng Zhang

Frontiers in immunology 15:1342647 PubMed38550593

2024

STING agonist inflames the cervical cancer immune microenvironment and overcomes anti-PD-1 therapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Tianye Li,Weijiang Zhang,Mengke Niu,Yuze Wu,Xinyue Deng,Jianwei Zhou

Stem cells international 2023:6256115 PubMed36970596

2023

Intravenously Administered Human Umbilical Cord-Derived Mesenchymal Stem Cell (HucMSC) Improves Cardiac Performance following Infarction via Immune Modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoting Liang,Jing Liu,Mimi Li,Fang Lin,Rulin Zhuang,Qingshu Meng,Xiaoxue Ma,Yuanfeng Xin,Xin Gong,Zhiying He,Wei Han,Xiaohui Zhou,Zhongmin Liu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2206344 PubMed36721037

2023

Loss of RPA1 Impairs Peripheral T Cell Homeostasis and Exacerbates Inflammatory Damage through Triggering T Cell Necroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Song,Xin Zhang,Yue Yin,Mengfan Guo,Xuyang Zhao,Likun Wang,Caixia Ren,Yuxin Yin,Xuehui Zhang,Xuliang Deng,Dan Lu

Frontiers in microbiology 13:1042923 PubMed36532416

2022

Chronic alcohol-induced dysbiosis of the gut microbiota and gut metabolites impairs sperm quality in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Li,Ningshan Li,Qudong Lu,Jun Yang,Jiang Zhao,Qiong Zhu,Shanhong Yi,Weihua Fu,Tingting Luo,Jiawei Tang,Yi Zhang,Guoliang Yang,Zheng Liu,Jie Xu,Wei Chen,Jingzhen Zhu

Stem cell research & therapy 13:247 PubMed35690805

2022

Intramyocardial injected human umbilical cord-derived mesenchymal stem cells (HucMSCs) contribute to the recovery of cardiac function and the migration of CD4 T cells into the infarcted heart via CCL5/CCR5 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Liu,Xiaoting Liang,Mimi Li,Fang Lin,Xiaoxue Ma,Yuanfeng Xin,Qingshu Meng,Rulin Zhuang,Qingliu Zhang,Wei Han,Ling Gao,Zhiying He,Xiaohui Zhou,Zhongmin Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD3 epsilon antibody [EPR22667-12]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)

Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)

Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)

Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)

Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (12)

Product promise