Rabbit Recombinant Monoclonal CD3E antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse samples. Cited in 6 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-ab231775--immunocytochemistry-immunofluorescence-img108834.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)")
![Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-ab231775--immunoprecipitation-img4091.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-ab231775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57899.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)")
![Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-ab231775--western-blot-img84638.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-ab231775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109935.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] (AB231775)")
Publications
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- Stem cells international 2023:62561152023Intravenously Administered Human Umbilical Cord-Derived Mesenchymal Stem Cell (HucMSC) Improves Cardiac Performance following Infarction via Immune Modulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoting Liang,Jing Liu,Mimi Li,Fang Lin,Rulin Zhuang,Qingshu Meng,Xiaoxue Ma,Yuanfeng Xin,Xin Gong,Zhiying He,Wei Han,Xiaohui Zhou,Zhongmin LiuPubMed 36970596
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e22063442023Loss of RPA1 Impairs Peripheral T Cell Homeostasis and Exacerbates Inflammatory Damage through Triggering T Cell Necroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia Song,Xin Zhang,Yue Yin,Mengfan Guo,Xuyang Zhao,Likun Wang,Caixia Ren,Yuxin Yin,Xuehui Zhang,Xuliang Deng,Dan LuPubMed 36721037
- Frontiers in microbiology 13:10429232022Chronic alcohol-induced dysbiosis of the gut microbiota and gut metabolites impairs sperm quality in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Li,Ningshan Li,Qudong Lu,Jun Yang,Jiang Zhao,Qiong Zhu,Shanhong Yi,Weihua Fu,Tingting Luo,Jiawei Tang,Yi Zhang,Guoliang Yang,Zheng Liu,Jie Xu,Wei Chen,Jingzhen ZhuPubMed 36532416
- Stem cell research & therapy 13:2472022Intramyocardial injected human umbilical cord-derived mesenchymal stem cells (HucMSCs) contribute to the recovery of cardiac function and the migration of CD4 T cells into the infarcted heart via CCL5/CCR5 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Liu,Xiaoting Liang,Mimi Li,Fang Lin,Xiaoxue Ma,Yuanfeng Xin,Qingshu Meng,Rulin Zhuang,Qingliu Zhang,Wei Han,Ling Gao,Zhiying He,Xiaohui Zhou,Zhongmin LiuPubMed 35690805
- Frontiers in immunology 12:7385862021VISTA Blockade Aggravates Bone Loss in Experimental Murine Apical Periodontitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFuhua Yang,Yifei Zhang,Zhi Chen,Lu ZhangPubMed 34691045
- Journal of hematology & oncology 14:1462021Combine and conquer: manganese synergizing anti-TGF-β/PD-L1 bispecific antibody YM101 to overcome immunotherapy resistance in non-inflamed cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Yi,Mengke Niu,Jing Zhang,Shiyu Li,Shuangli Zhu,Yongxiang Yan,Ning Li,Pengfei Zhou,Qian Chu,Kongming WuPubMed 34526097
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Mouse | Tested | Not recommended | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/30 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed:19956738, PubMed:24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (By similarity).
Alternative names
CD3e, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, Cd3e
Recommended products
Rabbit Recombinant Monoclonal CD3E antibody. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR22667-12
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-ab231775--immunocytochemistry-immunofluorescence-img108834.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775)")
Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized EL4.IL2 (mouse lymphoma T lymphocyte cell line) cells labeling CD3 epsilon with ab231775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in EL4.IL2 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
![Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-ab231775--immunoprecipitation-img4091.jpg "Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775)")
Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775)
CD3 epsilon was immunoprecipitated from 0.35 mg of EL4.IL2 (mouse lymphoma T lymphocyte cell line) whole cell lysate with ab231775 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab231775 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as the secondary antibody at 1/5000 dilution.
Lane 1: EL4.IL2 whole cell lysate 10 μg (Input).
Lane 2: ab231775 IP in EL4.IL2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab231775 in EL4.IL2 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.All lanes: Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775)
Predicted band size: 23 kDa
Observed band size: 22 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-ab231775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57899.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD3 epsilon with ab231775 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the mouse spleen (PMID: 18648532) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-ab231775--western-blot-img84638.jpg "Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775)")
Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775) at 1/1000 dilution
All lanes: EL4.IL2 (mouse lymphoma T lymphocyte cell line) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 23 kDa
Observed band size: 22 kDa
Exposure time: 158s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-ab231775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109935.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CD3 epsilon with ab231775 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the T lymphocytes on mouse lung (PMID: 22888136) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-ab231775--western-blot-img74639.jpg "Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775)")
Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775)
Blocking and dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 1387664).
All lanes: Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775) at 1/1000 dilution
All lanes: Mouse thymus lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 18 kDa, 22 kDa
Exposure time: 15s
![Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-ab231775--western-blot-img74639.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD3 epsilon antibody [EPR22667-12] (ab231775)")
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