Rabbit Recombinant Monoclonal CD3E antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (AB255697), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-bsa-and-azide-free-ab255697--immunocytochemistry-immunofluorescence-img108834.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (AB255697)")
![Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (AB255697), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-bsa-and-azide-free-ab255697--immunoprecipitation-img4091.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (AB255697)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (AB255697), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-bsa-and-azide-free-ab255697--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57899.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (AB255697)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (AB255697), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-bsa-and-azide-free-ab255697--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109935.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (AB255697)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|
| Mouse | Tested | Not recommended | Expected | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed:19956738, PubMed:24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (By similarity).
Alternative names
CD3e, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, Cd3e
Recommended products
Rabbit Recombinant Monoclonal CD3E antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22667-12
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab255697 is the carrier-free version of Anti-CD3 epsilon antibody [EPR22667-12] ab231775.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-bsa-and-azide-free-ab255697--immunocytochemistry-immunofluorescence-img108834.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)")
Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized EL4.IL2 (mouse lymphoma T lymphocyte cell line) cells labeling CD3 epsilon with Anti-CD3 epsilon antibody [EPR22667-12] ab231775 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and cytoplasmic staining in EL4.IL2 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red).
Antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 epsilon antibody [EPR22667-12] ab231775).
![Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-bsa-and-azide-free-ab255697--immunoprecipitation-img4091.jpg "Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)")
Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)
CD3 epsilon was immunoprecipitated from 0.35 mg of EL4.IL2 (mouse lymphoma T lymphocyte cell line) whole cell lysate with Anti-CD3 epsilon antibody [EPR22667-12] ab231775 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-CD3 epsilon antibody [EPR22667-12] ab231775 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: EL4.IL2 whole cell lysate [amount of lysate 10 μg (Input).
Lane 2: Anti-CD3 epsilon antibody [EPR22667-12] ab231775 IP in EL4.IL2 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD3 epsilon antibody [EPR22667-12] ab231775 in EL4.IL2 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 epsilon antibody [EPR22667-12] ab231775).
All lanes: Immunoprecipitation - Anti-CD3 epsilon antibody [EPR22667-12] (Anti-CD3 epsilon antibody [EPR22667-12] ab231775)
Predicted band size: 23 kDa
Observed band size: 22 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-bsa-and-azide-free-ab255697--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57899.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD3 epsilon with Anti-CD3 epsilon antibody [EPR22667-12] ab231775 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the mouse spleen (PMID: 18648532) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 epsilon antibody [EPR22667-12] ab231775).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697), expandable thumbnail](https://content.abcam.com/products/images/cd3-epsilon-antibody-epr22667-12-bsa-and-azide-free-ab255697--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109935.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 epsilon antibody [EPR22667-12] - BSA and Azide free (ab255697)
Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CD3 epsilon with Anti-CD3 epsilon antibody [EPR22667-12] ab231775 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on the T lymphocytes on mouse lung (PMID: 22888136) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 epsilon antibody [EPR22667-12] ab231775).
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