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Rabbit Recombinant Monoclonal CD3E antibody. Suitable for ICC/IF, IP, Flow Cyt, WB and reacts with Human samples. Cited in 1 publication.

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Images

Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628), expandable thumbnail
  • Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628), expandable thumbnail
  • Immunoprecipitation - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628), expandable thumbnail
  • Western blot - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Tested
Tested
Tested
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/500
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
1/10000 - 1/50000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed:10384095, PubMed:26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed:38614099).

Alternative names

CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain

Recommended products

Rabbit Recombinant Monoclonal CD3E antibody. Suitable for ICC/IF, IP, Flow Cyt, WB and reacts with Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR5361(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628), expandable thumbnail

    Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)

    Flow cytometric analysis of Human peripheral blood mononuclear cells (PBMC) labelling CD3 with ab133628 at 1/100 dilution (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (left) and an unlabelled control (cells without incubation with primary antibody and secondary antibody). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628), expandable thumbnail

    Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)

    Flow cytometry overlay histogram of Jurkat (Human T cell leukemia T lymphocyte) cells labelling CD3 epsilon with ab133628 at 1/100 dilution (red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed Jurkat (human T cell leukemia T lymphocyte) cells labelling CD3 epsilon with ab133628 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cells. Counterstained with Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1:200 dilution.
    The negative controls was Raji cell line.

  • Immunoprecipitation - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628), expandable thumbnail

    Immunoprecipitation - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)

    Purified ab133628 at 1/50 dilution (2μg) immunoprecipitating CD3 epsilon in Hut-78 whole cell lysate.
    Lane 1 (input): Hut-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate 10μg
    Lane 2 (+): ab133628 + Hut-78 whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab133628 in Hut-78 whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 23 kDa

    All lanes: Immunoprecipitation - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)

    Predicted band size: 23 kDa

  • Western blot - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628), expandable thumbnail

    Western blot - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)

    CD3 epsilon Western blot staining using rabbit Anti-CD3 epsilon antibody

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628) at 1/1000 dilution

    Lane 1: Human peripheral blood mononuclear cell (PBMC) lysate at 20 µg

    Lane 2: Human thymus tissue lysate at 20 µg

    Lane 3: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 23 kDa

    Observed band size: 23 kDa

    Exposure time: 10s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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