AB133628

Anti-CD3 epsilon antibody [EPR5361(2)]

20ul selling size
RabMAb
Recombinant
Lab Essentials
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal CD3E antibody. Suitable for ICC/IF, IP, Flow Cyt, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain

5 Images

Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628)

Flow Cyt

Lab

Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628)

Flow cytometric analysis of Human peripheral blood mononuclear cells (PBMC) labelling CD3 with ab133628 at 1/100 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) (left) and an unlabelled control (cells without incubation with primary antibody and secondary antibody). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

Flow Cyt

Lab

Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628)

Flow cytometry overlay histogram of Jurkat (Human T cell leukemia T lymphocyte) cells labelling CD3 epsilon with ab133628 at 1/100 dilution (red) compared with a Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Jurkat (human T cell leukemia T lymphocyte) cells labelling CD3 epsilon with ab133628 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cells. Counterstained with Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1 : 200 dilution.
The negative controls was Raji cell line.

Lab

Immunoprecipitation - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628)

Purified ab133628 at 1/50 dilution (2μg) immunoprecipitating CD3 epsilon in Hut-78 whole cell lysate.
Lane 1 (input) : Hut-78 (Human Sezary syndrome cutaneous T lymphocyte) whole cell lysate 10μg
Lane 2 (+) : ab133628 + Hut-78 whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab133628 in Hut-78 whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 23 kDa

All lanes:

Immunoprecipitation - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628)

Predicted band size: 23 kDa

false

Supplier Data

Western blot - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CD3 epsilon antibody [EPR5361(2)] (ab133628) at 1/1000 dilution

Lane 1:

Human peripheral blood mononuclear cell (PBMC) lysate at 20 µg

Lane 2:

Human thymus tissue lysate at 20 µg

Lane 3:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 23 kDa

Observed band size: 23 kDa

false

Exposure time: 10s

Carrier free

Anti-CD3 epsilon antibody [EPR5361(2)] - BSA and Azide free
660 APC

APC Anti-CD3 epsilon antibody [EPR5361(2)]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CD3 epsilon antibody [EPR5361(2)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-CD3 epsilon antibody [EPR5361(2)]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-CD3 epsilon antibody [EPR5361(2)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-CD3 epsilon antibody [EPR5361(2)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-CD3 epsilon antibody [EPR5361(2)]
578 PE

PE Anti-CD3 epsilon antibody [EPR5361(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5361(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100 - 1/500", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response (PubMed : 15294938, PubMed : 15546002, PubMed : 2470098, PubMed : 40592325, PubMed : 8490660). When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z (PubMed : 2470098, PubMed : 40592325). All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain (PubMed : 2470098, PubMed : 40592325). Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098, PubMed : 40592325). CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (By similarity). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (By similarity). Also participates in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed : 10384095, PubMed : 26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1 (PubMed : 38614099). Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed : 12110186, PubMed : 38614099).

See full target information CD3E

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 114:E5891-E5899 PubMed28659468

2017

Ionic CD3-Lck interaction regulates the initiation of T-cell receptor signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lunyi Li,Xingdong Guo,Xiaoshan Shi,Changting Li,Wei Wu,Chengsong Yan,Haopeng Wang,Hua Li,Chenqi Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD3 epsilon antibody [EPR5361(2)]

Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628)

Flow Cytometry - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628)

Immunocytochemistry/ Immunofluorescence - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628)

Immunoprecipitation - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628)

Western blot - Anti-CD3 epsilon antibody [EPR5361(2)] (AB133628)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Storage information

Product protocols

Target data

Publications (1)

Product promise