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AB210317

Anti-CD3 epsilon antibody [OKT3] - Low endotoxin, Azide free

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(1 Publication)

Mouse Monoclonal CD3E antibody. Carrier free. Suitable for Flow Cyt, IHC-P, FuncS (Act), IHC-Fr and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD3e, T3E, CD3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-CD3 antibody [OKT3]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-CD3 antibody [OKT3]

  • redFluor™ 710

    redFluor™ 710 Anti-CD3 antibody [OKT3]

  • 660 APC

    APC Anti-CD3 epsilon antibody [OKT3]

  • Biotin

    Biotin Anti-CD3 antibody [OKT3]

  • 519 FITC

    FITC Anti-CD3 epsilon antibody [OKT3]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OKT3

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, FuncS (Act), Flow Cyt, IHC-Fr

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FuncSAct" : {"fullname" : "Functional Studies (Act)", "shortname":"FuncS (Act)"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FuncSAct-species-checked": "guaranteed", "FuncSAct-species-dilution-info": "", "FuncSAct-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Chimpanzee": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FuncSAct-species-checked": "predicted", "FuncSAct-species-dilution-info": "", "FuncSAct-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Product details

ab210317 is a widely used phenotypic marker for human T cells. In addition, as the CD3 epsilon subunit of the TCR complex contains intracellular signaling domains, binding of ab210317 to CD3 epsilon can induce cell activation.

Endotoxin Level: Less than or equal to 0.01 EU/ug, as determined by the LaL assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from tissue culture supernatant. >90% pure as determined by SDS-PAGE analysis
Storage buffer
pH: 7.2 Constituents: 0.87% Sodium chloride, 0.16% Sodium dihydrogen phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed : 10384095, PubMed : 26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed : 38614099).
See full target information CD3E
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 25:271 PubMed37206552

2023

Changes in the balance of Th17/Treg cells and oxidative stress markers in patients with HIV‑associated pulmonary tuberculosis who develop IRIS.

Applications

Unspecified application

Species

Unspecified reactive species

Long Wen,Lei Shi,Shan-Shan Wan,Tao Xu,Lei Zhang,Zhi-Guo Zhou
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Product promise

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