AB119827

Anti-CD3 zeta antibody [H146-968] - BSA and Azide free

(6 Reviews)

(7 Publications)

Armenian hamster Monoclonal CD3 zeta antibody. Carrier free. Suitable for ICC, IP, Flow Cyt, WB and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Mouse Cd247 aa 150 to C-terminus.

View Alternative Names

CD247, CD3Z, T3Z, TCRZ, T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain

2 Images

Supplier Data

Western blot - Anti-CD3 zeta antibody [H146-968] - BSA and Azide free (AB119827)

All lanes:

Western blot - Anti-CD3 zeta antibody [H146-968] - BSA and Azide free (ab119827)

Lane 1:

Murine splenocytes

Lane 2:

MOLT-4 cell line

Secondary

Lane 1:

conjugated goat-anti-armenian hamster

Lane 2:

Alexa Fluor® 790-conjugated goat-anti-armenian hamster

Predicted band size: 19 kDa

false

Supplier Data

Western blot - Anti-CD3 zeta antibody [H146-968] - BSA and Azide free (AB119827)

All lanes:

Western blot - Anti-CD3 zeta antibody [H146-968] - BSA and Azide free (ab119827) at 2 µg/mL

All lanes:

Murine Splenocytes

Predicted band size: 19 kDa

false

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

H146-968

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

Flow Cyt, WB, IP, ICC

applications

Immunogen

Synthetic Peptide within Mouse Cd247 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P24161

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/armenian-hamster-igg-monoclonal-htk888-isotype-control-ab18479'>ab18479</a> - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/armenian-hamster-igg-monoclonal-htk888-isotype-control-ab18479'>ab18479</a> - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab119827 is purified from cell culture supernatant by protein A affinity chromatography and 0.2 μm filter sterilized. Purity is > 95% by SDS-PAGE.

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 1384049, PubMed : 1385158, PubMed : 2470098, PubMed : 7509083). CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed : 7509083). Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity).

See full target information CD247

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Clinical cancer research : an official journal of the American Association for Cancer Research 27:3757-3771 PubMed33811153

2021

A Pan-Histone Deacetylase Inhibitor Enhances the Antitumor Activity of B7-H3-Specific CAR T Cells in Solid Tumors.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyuan Lei,Zhanpeng Ou,Zhaohui Yang,Jianglong Zhong,Yanliang Zhu,Jing Tian,Jiannan Wu,Heran Deng,Xinyu Lin,Yu Peng,Bowen Li,Lile He,Zhiming Tu,Weixiong Chen,Qunxing Li,Niu Liu,Hanqing Zhang,Zhangsong Wang,Zezhen Fang,Teppei Yamada,Xiaobin Lv,Tian Tian,Guokai Pan,Fan Wu,Liping Xiao,Lizao Zhang,Tingting Cai,Xinhui Wang,Bakhos A Tannous,Jinsong Li,Filippos Kontos,Soldano Ferrone,Song Fan

Journal for immunotherapy of cancer 7:209 PubMed31391096

2019

PD-1 silencing impairs the anti-tumor function of chimeric antigen receptor modified T cells by inhibiting proliferation activity.

Applications

Unspecified application

Species

Unspecified reactive species

Jianshu Wei,Can Luo,Yao Wang,Yelei Guo,Hanren Dai,Chuan Tong,Dongdong Ti,Zhiqiang Wu,Weidong Han

Nature immunology 19:1083-1092 PubMed30224819

2018

NK cell receptor NKG2D sets activation threshold for the NCR1 receptor early in NK cell development.

Applications

Unspecified application

Species

Unspecified reactive species

Vedrana Jelenčić,Marko Šestan,Inga Kavazović,Maja Lenartić,Sonja Marinović,Tim D Holmes,Michaela Prchal-Murphy,Berislav Lisnić,Veronika Sexl,Yenan T Bryceson,Felix M Wensveen,Bojan Polić

Journal of immunology (Baltimore, Md. : 1950) 165:5062-8 PubMed11046036

2000

Regulation of cell surface expression of CTLA-4 by secretion of CTLA-4-containing lysosomes upon activation of CD4+ T cells.

Applications

Unspecified application

Species

Unspecified reactive species

T Iida,H Ohno,C Nakaseko,M Sakuma,M Takeda-Ezaki,H Arase,E Kominami,T Fujisawa,T Saito

International immunology 11:1395-401 PubMed10464160

1999

Translocation of tyrosine-phosphorylated TCRzeta chain to glycolipid-enriched membrane domains upon T cell activation.

Applications

Unspecified application

Species

Unspecified reactive species

A Kosugi,S Saitoh,S Noda,K Yasuda,F Hayashi,M Ogata,T Hamaoka

The Journal of experimental medicine 186:1933-8 PubMed9382891

1998

Signaling efficiency of the T cell receptor controlled by a single amino acid in the beta chain constant region.

Applications

Unspecified application

Species

Unspecified reactive species

B T Bäckström,B T Hausmann,E Palmer

Journal of immunology (Baltimore, Md. : 1950) 153:1563-80 PubMed7519206

1994

Developmental regulation of the TCR zeta-chain. Differential expression and tyrosine phosphorylation of the TCR zeta-chain in resting immature and mature T lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

M M Rozdzial,R T Kubo,S L Turner,T H Finkel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CD3 zeta antibody [H146-968] - BSA and Azide free

Western blot - Anti-CD3 zeta antibody [H146-968] - BSA and Azide free (AB119827)

Western blot - Anti-CD3 zeta antibody [H146-968] - BSA and Azide free (AB119827)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

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