Rabbit Recombinant Monoclonal CD3 zeta phospho Y142 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (AB245765), expandable thumbnail](https://content.abcam.com/products/images/cd3-zeta-phospho-y142-antibody-ep2652y-bsa-and-azide-free-ab245765--western-blot-img15137.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (AB245765)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (AB245765), expandable thumbnail](https://content.abcam.com/products/images/cd3-zeta-phospho-y142-antibody-ep2652y-bsa-and-azide-free-ab245765--immunocytochemistry-immunofluorescence-img84545.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (AB245765)")
![Immunoprecipitation - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (AB245765), expandable thumbnail](https://content.abcam.com/products/images/cd3-zeta-phospho-y142-antibody-ep2652y-bsa-and-azide-free-ab245765--immunoprecipitation-img4721.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (AB245765)")
![Flow Cytometry (Intracellular) - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (AB245765), expandable thumbnail](https://content.abcam.com/products/images/cd3-zeta-phospho-y142-antibody-ep2652y-bsa-and-azide-free-ab245765--flow-cytometry-intracellular-img25350.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (AB245765)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
11 products for Alternative Product
8 products for Alternative Version
Target data
Function
Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098, PubMed:7509083). CD3Z ITAMs phosphorylation creates multiple docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (PubMed:7509083). Plays an important role in intrathymic T-cell differentiation. Additionally, participates in the activity-dependent synapse formation of retinal ganglion cells (RGCs) in both the retina and dorsal lateral geniculate nucleus (dLGN) (By similarity).
Alternative names
T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD3Z, T3Z, TCRZ, CD247
Recommended products
Rabbit Recombinant Monoclonal CD3 zeta phospho Y142 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Alternative names
T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD3Z, T3Z, TCRZ, CD247
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP265(2)Y
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab245765 is the carrier-free version of Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] ab68235.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765), expandable thumbnail](https://content.abcam.com/products/images/cd3-zeta-phospho-y142-antibody-ep2652y-bsa-and-azide-free-ab245765--western-blot-img15137.jpg "Western blot - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)")
Western blot - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] ab68235).
All lanes: Western blot - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765) at 1/1000 dilution
Lane 1: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 15 µg
Lane 2: Jurkat (Human T cell leukemia T lymphocyte) treated with 50mM pervanadate for 5 minutes whole cell lysates at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 19 kDa
Observed band size: 19 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765), expandable thumbnail](https://content.abcam.com/products/images/cd3-zeta-phospho-y142-antibody-ep2652y-bsa-and-azide-free-ab245765--immunocytochemistry-immunofluorescence-img84545.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)")
Immunocytochemistry/ Immunofluorescence - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)
Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) treated with 1mM pervanadate for 30 minutes cells labeling CD3 zeta with purified Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] ab68235 at 1/500 dilution (0.2 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] ab68235).
![Immunoprecipitation - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765), expandable thumbnail](https://content.abcam.com/products/images/cd3-zeta-phospho-y142-antibody-ep2652y-bsa-and-azide-free-ab245765--immunoprecipitation-img4721.jpg "Immunoprecipitation - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)")
Immunoprecipitation - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)
Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] ab68235 (purified) at 1/20 dilution (0.5ug) immunoprecipitating CD3 zeta in Jurkat treated with 50nM pervanadate for 5 minutes whole cell lysate whole cell lysate. Jurkat (Human T cell leukemia T lymphocyte) treated with 50nM pervanadate for 5 minutes whole cell lysate 10ug
Lane 2 (+): Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] ab68235 & Jurkat treated with 50nM pervanadate for 5 minutes whole cell lysate whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] ab68235 in Jurkat treated with 50nM pervanadate for 5 minutes whole cell lysate
For western blotting, VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/2000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] ab68235).All lanes: Immunoprecipitation - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] (Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] ab68235)
Predicted band size: 19 kDa
![Flow Cytometry (Intracellular) - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765), expandable thumbnail](https://content.abcam.com/products/images/cd3-zeta-phospho-y142-antibody-ep2652y-bsa-and-azide-free-ab245765--flow-cytometry-intracellular-img25350.jpg "Flow Cytometry (Intracellular) - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)")
Flow Cytometry (Intracellular) - Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] - BSA and Azide free (ab245765)
Intracellular Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) treated with 50nM pervanadate for 5 minutes cells labeling CD3 zeta with purified Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] ab68235 at 1/120 dilution (1μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). Untreated Jurkat cells (Green).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CD3 zeta (phospho Y142) antibody [EP265(2)Y] ab68235).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com