JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB871

Anti-CD30 antibody [Ber-H2], prediluted

Be the first to review this product! Submit a review

|

(2 Publications)

Mouse Monoclonal CD30 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CD30, D1S166E, TNFRSF8, Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (AB871)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (AB871)

IHC image of ab871 staining in Hodgkin lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab871, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (AB871)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [Ber-H2], prediluted (AB871)

Formalin-fixed, paraffin-embeded human Hodgkin's lymphoma tissue stained for CD30 using ab871 in immunohistochemical analysis. Antigen retrieval with EDTA buffer pH 8.0

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Ber-H2

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Specificity

This antibody reacts with mononuclear cells in Hodgkin lymphoma, Reed-Sternberg cells and most anaplastic large cell lymphomas. It stains large cells localized around the B cell in the lymphoid tissues. This antibody stains the cell surface and the golgi region in the cells.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Enzymatic predigestion with proteolytic enzymes may increase staining intensity. Although not required, microwave treatment will considerably enhance the signal.</p>" } } }

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Receptor for TNFSF8/CD30L (PubMed : 8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed : 8999898).
See full target information TNFRSF8

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Anatomia, histologia, embryologia 50:307-315 PubMed33219562

2020

Seasonal morphology and immunoreactivity of cytokeratin and atrial natriuretic peptide in dromedary camel poll glands.

Applications

Unspecified application

Species

Unspecified reactive species

Zarroug Hassan Ibrahim,Khalid Ali Al-Kheraije

Science advances 5:eaax5851 PubMed31633026

2019

MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure.

Applications

Mass Cytometry

Species

Unspecified reactive species

Leeat Keren,Marc Bosse,Steve Thompson,Tyler Risom,Kausalia Vijayaragavan,Erin McCaffrey,Diana Marquez,Roshan Angoshtari,Noah F Greenwald,Harris Fienberg,Jennifer Wang,Neeraja Kambham,David Kirkwood,Garry Nolan,Thomas J Montine,Stephen J Galli,Robert West,Sean C Bendall,Michael Angelo
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com