Rabbit Recombinant Monoclonal CD30 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TNFRSF8 aa 1-400.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [BLR055F] (AB252561), expandable thumbnail](https://content.abcam.com/products/images/cd30-antibody-blr055f-ab252561--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48364.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [BLR055F] (AB252561)")
![Immunocytochemistry - Anti-CD30 antibody [BLR055F] (AB252561), expandable thumbnail](https://content.abcam.com/products/images/cd30-antibody-blr055f-ab252561--immunocytochemistry-img124295.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry - Anti-CD30 antibody [BLR055F] (AB252561)")
![Western blot - Anti-CD30 antibody [BLR055F] (AB252561), expandable thumbnail](https://content.abcam.com/products/images/cd30-antibody-blr055f-ab252561--western-blot-img126706.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CD30 antibody [BLR055F] (AB252561)")
![Immunoprecipitation - Anti-CD30 antibody [BLR055F] (AB252561), expandable thumbnail](https://content.abcam.com/products/images/cd30-antibody-blr055f-ab252561--immunoprecipitation-img70060.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CD30 antibody [BLR055F] (AB252561)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human TNFRSF8 aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link P28908
Abcam Recommends
Reactivity data
Select an application| IHC-P | ICC | IP | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 5 μL / mg of lysate |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Receptor for TNFSF8/CD30L (PubMed:8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed:8999898).
Alternative names
CD30, D1S166E, TNFRSF8, Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30
Recommended products
Rabbit Recombinant Monoclonal CD30 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TNFRSF8 aa 1-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 99% Borate buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human TNFRSF8 aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link P28908
- Clone number
- BLR055F
- Concentration
- Purification notes
Purified from cell culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is sold under License from Bethyl Laboratories, Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [BLR055F] (ab252561), expandable thumbnail](https://content.abcam.com/products/images/cd30-antibody-blr055f-ab252561--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48364.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [BLR055F] (ab252561)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [BLR055F] (ab252561)
Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD30 using ab252561 at 1/250 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: DAB staining.
![Immunocytochemistry - Anti-CD30 antibody [BLR055F] (ab252561), expandable thumbnail](https://content.abcam.com/products/images/cd30-antibody-blr055f-ab252561--immunocytochemistry-img124295.jpg "Immunocytochemistry - Anti-CD30 antibody [BLR055F] (ab252561)")
Immunocytochemistry - Anti-CD30 antibody [BLR055F] (ab252561)
Formalin-fixed, paraffin-embedded sections of SR (human large cell immunoblastic lymphoma cell line) cells stained for CD30 using ab252561 at 1/250 dilution in immunohistochemical analysis. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: DAB staining.
![Western blot - Anti-CD30 antibody [BLR055F] (ab252561), expandable thumbnail](https://content.abcam.com/products/images/cd30-antibody-blr055f-ab252561--western-blot-img126706.jpg "Western blot - Anti-CD30 antibody [BLR055F] (ab252561)")
Western blot - Anti-CD30 antibody [BLR055F] (ab252561)
Lysates prepared in NETN buffer.
All lanes: Western blot - Anti-CD30 antibody [BLR055F] (ab252561) at 1/1000 dilution
Lane 1: MJ (human cutaneous T cell lymphoma cell line) whole cell lysate at 50 µg
Lane 2: SR (human large cell immunoblastic lymphoma cell line) whole cell lysate at 50 µg
Lane 3: C5/MJ (human leukemia T cell lymphocyte cell line) whole cell lysate at 50 µg
Lane 4: GaMG (human cell line) whole cell lysate at 50 µg
Lane 5: U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate at 50 µg
Lane 6: A549 (human lung carcinoma cell line) whole cell lysate at 50 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 64 kDa
Exposure time: 30s
![Immunoprecipitation - Anti-CD30 antibody [BLR055F] (ab252561), expandable thumbnail](https://content.abcam.com/products/images/cd30-antibody-blr055f-ab252561--immunoprecipitation-img70060.jpg "Immunoprecipitation - Anti-CD30 antibody [BLR055F] (ab252561)")
Immunoprecipitation - Anti-CD30 antibody [BLR055F] (ab252561)
CD30 was immunoprecipitated from MJ (human cutaneous T cell lymphoma cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab252561 at 5 μL per reaction. Western blot was performed from the immunoprecipitate using ab252561 at 1/1000 dilution.
Lane 1: ab252561 IP in MJ whole cell lysate.
Lane 2: Control IgG IP in MJ whole cell lysate.Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared in NETN buffer.
All lanes: Immunoprecipitation - Anti-CD30 antibody [BLR055F] (ab252561)
Predicted band size: 64 kDa
Downloads
Product protocols
- Visit the general protocols
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