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AB252561

Anti-CD30 antibody [BLR055F]

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(2 Publications)

Rabbit Recombinant Monoclonal CD30 antibody. Suitable for IHC-P, ICC, IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TNFRSF8 aa 1-400.

View Alternative Names

CD30, D1S166E, TNFRSF8, Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [BLR055F] (AB252561)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD30 antibody [BLR055F] (AB252561)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD30 using ab252561 at 1/250 dilution in immunohistochemical analysis. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : DAB staining.

Immunocytochemistry - Anti-CD30 antibody [BLR055F] (AB252561)
  • ICC

Supplier Data

Immunocytochemistry - Anti-CD30 antibody [BLR055F] (AB252561)

Formalin-fixed, paraffin-embedded sections of SR (human large cell immunoblastic lymphoma cell line) cells stained for CD30 using ab252561 at 1/250 dilution in immunohistochemical analysis. Secondary : HRP-conjugated goat anti-rabbit IgG. Detection : DAB staining.

Immunoprecipitation - Anti-CD30 antibody [BLR055F] (AB252561)
  • IP

Supplier Data

Immunoprecipitation - Anti-CD30 antibody [BLR055F] (AB252561)

CD30 was immunoprecipitated from MJ (human cutaneous T cell lymphoma cell line) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab252561 at 5 μL per reaction. Western blot was performed from the immunoprecipitate using ab252561 at 1/1000 dilution.

Lane 1 : ab252561 IP in MJ whole cell lysate.
Lane 2 : Control IgG IP in MJ whole cell lysate.

Detection : Chemiluminescence with exposure time of 30 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-CD30 antibody [BLR055F] (ab252561)

Predicted band size: 64 kDa

false

Western blot - Anti-CD30 antibody [BLR055F] (AB252561)
  • WB

Supplier Data

Western blot - Anti-CD30 antibody [BLR055F] (AB252561)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-CD30 antibody [BLR055F] (ab252561) at 1/1000 dilution

Lane 1:

MJ (human cutaneous T cell lymphoma cell line) whole cell lysate at 50 µg

Lane 2:

SR (human large cell immunoblastic lymphoma cell line) whole cell lysate at 50 µg

Lane 3:

C5/MJ (human leukemia T cell lymphocyte cell line) whole cell lysate at 50 µg

Lane 4:

GaMG (human cell line) whole cell lysate at 50 µg

Lane 5:

U-2 OS (human bone osteosarcoma epithelial cell line) whole cell lysate at 50 µg

Lane 6:

A549 (human lung carcinoma cell line) whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 64 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR055F

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC, IHC-P, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human TNFRSF8 aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

P28908

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "<p>Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.</p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>5 μL / mg of lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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Properties and storage information

Form
Liquid
Purification notes
Purified from cell culture supernatant.
Storage buffer
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for TNFSF8/CD30L (PubMed : 8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed : 8999898).
See full target information TNFRSF8
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

NAR cancer 7:zcaf037 PubMed41064808

2025

Co-expression of CD30 and SLFN11 serves as a dual biomarker for the treatment of cutaneous T-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Takatoshi Shimauchi,Junko Murai,Manami Iwasaki,Pawit Phadungsaksawasdi,Toshiyuki Ojima,Kazuyasu Fujii,Tetsuya Honda

Theranostics 15:3207-3222 PubMed40083918

2025

Enhanced therapeutic efficacy of anti-PD-1 blockade by targeting LAMP2A inhibits lysosomal degradation of STING and TBK1.

Applications

Unspecified application

Species

Unspecified reactive species

Xueying Wang,Diekuo Zhang,Jiaqing Xiao,Lei Wang,Junrong Wang,Xiaoqiao Cui,Jiaqi Tan,Yong Liu,Susheng Miao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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