Rabbit Recombinant Monoclonal CD30 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.
Images
![Flow Cytometry - Anti-CD30 antibody [EPR24238-228] (AB271127), expandable thumbnail](https://content.abcam.com/products/images/cd30-antibody-epr24238-228-ab271127--flow-cytometry-img170695.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD30 antibody [EPR24238-228] (AB271127)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [EPR24238-228] (AB271127), expandable thumbnail](https://content.abcam.com/products/images/cd30-antibody-epr24238-228-ab271127--immunocytochemistry-immunofluorescence-img175438.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [EPR24238-228] (AB271127)")
![Flow Cytometry - Anti-CD30 antibody [EPR24238-228] (AB271127), expandable thumbnail](https://content.abcam.com/products/images/cd30-antibody-epr24238-228-ab271127--flow-cytometry-img168186.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CD30 antibody [EPR24238-228] (AB271127)")
![Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [EPR24238-228] (AB271127), expandable thumbnail](https://content.abcam.com/products/images/cd30-antibody-epr24238-228-ab271127--immunocytochemistry-immunofluorescence-img179852.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [EPR24238-228] (AB271127)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/60 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Receptor for TNFSF8/CD30L (PubMed:8391931). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (PubMed:8999898).
Alternative names
CD30, D1S166E, TNFRSF8, Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Ki-1 antigen, Lymphocyte activation antigen CD30
Recommended products
Rabbit Recombinant Monoclonal CD30 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR24238-228
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry - Anti-CD30 antibody [EPR24238-228] (ab271127), expandable thumbnail](https://content.abcam.com/products/images/cd30-antibody-epr24238-228-ab271127--flow-cytometry-img170695.jpg "Flow Cytometry - Anti-CD30 antibody [EPR24238-228] (ab271127)")
Flow Cytometry - Anti-CD30 antibody [EPR24238-228] (ab271127)
Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells (Left) and KARPAS-299 (Human anaplastic large cell lymphoma) cells (Right) labelling CD30 with ab271127 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab')2 Anti-Rabbit IgG (DyLight® 488, Goat F(ab')2 Anti-Rabbit IgG - H&L (DyLight® 488), pre-adsorbed ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.
Negative control: Hela (PMID:11004694).
![Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [EPR24238-228] (ab271127), expandable thumbnail](https://content.abcam.com/products/images/cd30-antibody-epr24238-228-ab271127--immunocytochemistry-immunofluorescence-img175438.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [EPR24238-228] (ab271127)")
Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [EPR24238-228] (ab271127)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized KARPAS-299 cells labelling CD30 with ab271127 at 1/1000 (0.606 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous staining in KARPAS-299 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Negative control: HeLa (PMID: 11004694).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Flow Cytometry - Anti-CD30 antibody [EPR24238-228] (ab271127), expandable thumbnail](https://content.abcam.com/products/images/cd30-antibody-epr24238-228-ab271127--flow-cytometry-img168186.jpg "Flow Cytometry - Anti-CD30 antibody [EPR24238-228] (ab271127)")
Flow Cytometry - Anti-CD30 antibody [EPR24238-228] (ab271127)
Flow cytometric analysis of HDLM-2 (Human Hodgkin lymphoma) cells labelling CD30 with ab271127 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab')2 Anti-Rabbit IgG (DyLight® 488, Goat F(ab')2 Anti-Rabbit IgG - H&L (DyLight® 488), pre-adsorbed ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.
![Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [EPR24238-228] (ab271127), expandable thumbnail](https://content.abcam.com/products/images/cd30-antibody-epr24238-228-ab271127--immunocytochemistry-immunofluorescence-img179852.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [EPR24238-228] (ab271127)")
Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [EPR24238-228] (ab271127)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HDLM-2 cells labelling CD30 with ab271127 at 1/1000 (0.606 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous staining in HDLM-2 cell line. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) preadsorbed at 1/1000 (2 ug/ml) dilution.
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Product protocols
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