Anti-CD30 antibody [EPR24238-228]

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD30 antibody [EPR24238-228] (AB271127)

Flow cytometric analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells (Left) and KARPAS-299 (Human anaplastic large cell lymphoma) cells (Right) labelling CD30 with ab271127 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab')2 Anti-Rabbit IgG (DyLight® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.

Negative control : Hela (PMID : 11004694).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [EPR24238-228] (AB271127)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized KARPAS-299 cells labelling CD30 with ab271127 at 1/1000 (0.606 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous staining in KARPAS-299 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Negative control : HeLa (PMID : 11004694).

Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) preadsorbed at 1/1000 (2 ug/ml) dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CD30 antibody [EPR24238-228] (AB271127)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HDLM-2 cells labelling CD30 with ab271127 at 1/1000 (0.606 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing membranous staining in HDLM-2 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150081) preadsorbed at 1/1000 (2 ug/ml) dilution.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD30 antibody [EPR24238-228] (AB271127)

Flow cytometric analysis of HDLM-2 (Human Hodgkin lymphoma) cells labelling CD30 with ab271127 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab')2 Anti-Rabbit IgG (DyLight® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.