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AB324907

Anti-CD30 antibody [mCD30.1]

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Armenian hamster Recombinant Monoclonal CD30 antibody. Suitable for Dot, Flow Cyt and reacts with Recombinant full length protein - Mouse, Mouse samples.

View Alternative Names

CD30, Tumor necrosis factor receptor superfamily member 8, CD30L receptor, Lymphocyte activation antigen CD30, Tnfrsf8

4 Images
Flow Cytometry - Anti-CD30 antibody [mCD30.1] (AB324907)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD30 antibody [mCD30.1] (AB324907)

Flow cytometric analysis of Mouse spleen cells treated with 1ug/ml of immobilized anti-CD3 and 10ug/ml anti-CD28 for 3 days (Green and Black) / Untreated control (Magenta and Grey) cells labelling CD30 with ab324907 at 1/100 dilution (1ug) (Magenta and Green) compared with a Armenian Hamster monoclonal IgG (Black and Grey) isotype control.

Goat anti-Armenian hamster IgG (Alexa Fluor® 488, ab173003) at 1/5000 dilution was used as the secondary antibody.

Gated on viable CD8 positive cells.

Flow Cytometry - Anti-CD30 antibody [mCD30.1] (AB324907)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD30 antibody [mCD30.1] (AB324907)

Flow cytometric analysis of Mouse spleen cells treated with 2.5ug/ml Concanavalin A for 72 hours (Green and Black) / Untreated control (Magenta and Grey) cells labelling CD30 with ab324907 at 1/100 dilution (1ug) (Magenta and Green) compared with a Armenian Hamster monoclonal IgG (Black and Grey) isotype control.

Goat anti-Armenian hamster IgG (Alexa Fluor® 488, ab173003) at 1/5000 dilution was used as the secondary antibody.

Gated on viable CD8 positive cells.

Flow Cytometry - Anti-CD30 antibody [mCD30.1] (AB324907)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CD30 antibody [mCD30.1] (AB324907)

Flow cytometric analysis of NIH/3T3 (mouse embryonic fibroblast, Left) / YAC-1 (mouse Moloney leukemia virus induced lymphoma lymphoblast, Right) cells labelling CD30 with ab324907 at 1/100 dilution (1ug) (Red) compared with a Armenian Hamster monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti-Armenian hamster IgG (Alexa Fluor® 488, ab173003) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Negative control : NIH/3T3.

Dot Blot - Anti-CD30 antibody [mCD30.1] (AB324907)
  • Dot

Supplier Data

Dot Blot - Anti-CD30 antibody [mCD30.1] (AB324907)

Dot blot analysis of CD30 using ab324907 at 1 : 1000 (0.998 ug/ml) followed by a Rabbit Anti-Armenian hamster IgG H&L (HRP) (ab5745) at 1 : 2000 dilution.

Lane1 : Recombinant Mouse CD30/TNFRSF8 Fc Chimera Protein

Exposure time : 10 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-CD30 antibody [mCD30.1] (ab324907) at 1/1000 dilution

All lanes:

Recombinant Mouse CD30/TNFRSF8 Fc Chimera Protein

Secondary

All lanes:

Dot Blot - Rabbit Anti-Armenian hamster IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/rabbit-armenian-hamster-igg-h-l-hrp-ab5745'>ab5745</a>) at 1/2000 dilution

false

Exposure time: 10s

  • Carrier free

    Anti-CD30 antibody [mCD30.1] - BSA and Azide free

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

mCD30.1

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Recombinant full length protein - Mouse": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for TNFSF8/CD30L (PubMed : 8543792). May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B (By similarity).
See full target information Tnfrsf8
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com